LOCUS       sequence-488605-       10596 bp    DNA     circular SYN 07-OCT-2025
DEFINITION  Rab10 GEF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10596)
  AUTHORS   Wang J, Nishiyama J, Parra-Bueno P, Okaz E, Oz G, Liu X, Watabe T, 
            Suponitsky-Kroyter I, McGraw TE, Szatmari EM, Yasuda R
  TITLE     Rab10 inactivation promotes AMPAR trafficking and spine enlargement 
            during long-term potentiation.
  JOURNAL   Elife. 2025 Sep 23;13:RP103879. doi: 10.7554/eLife.103879.
  PUBMED    40986440
REFERENCE   2  (bases 1 to 10596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2025-09-23"; volume: "13:RP103879. doi"; pages: " 
            10.7554/eLife.103879"
COMMENT     Sequence Label: pCAGGS-3HA-Dennd4c
FEATURES             Location/Qualifiers
     source          1..10596
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        400..676
                     /label=chicken beta-actin promoter
     intron          677..1697
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1617..1639
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1705..1724
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1767..1776
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1779..1805
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1809..1835
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1839..1865
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(7628..7647)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7693..7748
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7747..7766)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8109..8125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8109..8125)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8122..8144)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8133..8149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8157..8163,8164..8181,8182..8187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8202..8223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8282..8477
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8328..8463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8483..8617
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8520..8539)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8574..8593
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8685..8702)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8856..9444)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8936..8955)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9615..10406,10407..10475))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10238..10257
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10476..10580)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtgccacctg ggtcgatcga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
      421 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
      481 aattattttg tgcagcgatg ggggcggggg gggggggggg cgcgcgccag gcggggcggg
      541 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
      601 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
      661 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
      721 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
      781 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
      841 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
      901 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
      961 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     1021 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     1081 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     1141 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     1201 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     1261 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     1321 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     1381 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     1441 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     1501 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     1561 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     1621 cggcttctgg cgtgtgaccg gcggctctag ctagagcctc tgctaaccat gttcatgcct
     1681 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     1741 gcaaagaatt cgctagcgct accggtgcca ccatggcata cccttatgac gtaccagatt
     1801 acgcgggata tccctacgat gtccccgatt acgcgcgtta tccatatgac gtcccggact
     1861 atgccgaatt cctcgagggt accatagaag acaaaggacc tagagtgaca gactacttcg
     1921 ttgtggcagg tctcactgat acatccactc ttttggatca agaaataaat cgtacagata
     1981 ctaactcgat tggtcctaaa gctccaatca cagacattgc tgttattatc aaatcagctg
     2041 gggaaactgt acctgaaggc tacacctgtg tggaagccac tccatcagca ctccaagcaa
     2101 acttgaatta tggaagtctg aagagcccag agcttttcct gtgctacagg agagggagag
     2161 ataagccgcc ccttacagat attggcgtgc tatatgaagg gaaagaaagg cttatgccag
     2221 gatgtgaagt gatccaagcc acgccgtacg gccgctgtgc caatgtcaac aatagttcaa
     2281 ctacttctca aagaatcttt atcacctatc gaagggctcc cccagtgcgg tcccagaatt
     2341 ccttggccgt cactgatatc tgtgttatta taaccagtaa aggagaaacc cctccgcata
     2401 ctttctgcaa agtggataaa aatttaaact gtggcatgtg gggttccaac gtgtttctgt
     2461 gttacaagaa gtctgtgcct gcttcaaatg caatagcata taaggctggt ttgatttttc
     2521 gatatccgga agaagactat gagtcttttc cgctctcacc atctgtgcct ctcttctgcc
     2581 ttcctatggg agccactatt gaatgctggg atcctcaaat caaataccca cttcctgttt
     2641 tctcaacatt tgtcctgaca gggtcttcag ctgaaaaggt atatggagct gccatccagt
     2701 tttatgaacc ttactctcaa gaacgtctaa cagagaaaca acttacacag ctgggcctat
     2761 tgaccctcgt ggagaaaaga gtggtctcca aacccatcaa ttcaaacaag tgcatttgtt
     2821 tgctttcaca ctggcctttt tttgaagctt ttaagaattt tcttatgttt atctacaaag
     2881 tttctgtgtc tggaccacat cctcttccca ttgaaaagca catttcacat tttatgcaaa
     2941 atatcccttt tccttcacca caaagaccaa gaatcctcat ccagctatca gtccatgatg
     3001 catttatatt atcacagcca gtgtccacac ctttaccatt aagtggcgcc aactttagct
     3061 ccttgctgat gaatctgggt cctgagaact gcgcgacatt gctgctgctg gttttgctcg
     3121 agagtaagat tctgctgcac tctctgaggc cagcggtact aactggggta gccgaagctg
     3181 tcgttgctat gatctttcca tttcagtggc agtgcccata tatccccctc tgtcctcttt
     3241 cattggctgg agtgcttagt gcgcctttac catttatagt tggagtggac tcaaggtact
     3301 ttgatcttca tgacccacca caagatgttg tctgcattga cttggatacg aacacattat
     3361 atgtagctga tgaaagaaag aacataaact ggaagcagct tcccaaaagg ccgtgcaaga
     3421 gtctgcttgg caccctgagg aggctgtatc agcagctctg ctcagttcac cgaaaacctc
     3481 aagaaagttc agcaattgag atgactccaa ttgaagcaga ttactcttgg caaaaaaaga
     3541 tgacacaatt ggagatggaa attcaggaga catttttgcg atttatggcg tctattttaa
     3601 aagggtacag gtcatatctc agaccaatca cagaagctcc ttctaataaa gccacagctg
     3661 cggattcatt gtttgatcga cagggttttt tgaaaagtcg ggatcgtgcc tatacaaaat
     3721 tctatactct tttatccaaa acacagattt ttattcgttt cattgaagaa tgcagttttg
     3781 taagtgataa agatactgga ttggcatttt ttgatgattg catcgagaag ttgtttccag
     3841 ataaaggtgt agagagaaca gaaaaggttg acttggattc agcagaagac accagattga
     3901 tagagctaga tgattcacag agaagtgaac acactgtatt tataatgcca cctgagccgc
     3961 ctcctgatga tggaaataac ttatcacctc agtacagtta cacatacttt ccaagattgg
     4021 atcttaagct ttttgacagc ccacagaagc tgaaactgtg cttcaataga catcctcctg
     4081 ggagtagcat tacaaacagc cctgccctca tggctaaaag gactaaacag gagataaaaa
     4141 cagctcataa gttggcgaag agatgttata cgaatccacc acaatgggcc aagtatctat
     4201 tcagccactg ttacagtctg tggtttattt gtctcccagc ctatgttaga gtttctcacc
     4261 ctaaggtccg agcactccag caggcacatg atgtgcttgt taagatgcgg aagacagacg
     4321 tggatccact agatgaggtg tgctatcgag tagtcatgca gctttgtgga ctttgggtta
     4381 atcctgtttt agcagtgaga gtcttattcg agatgaaaac tgctagaata aaaccaaatg
     4441 ctatcactta tggctattac aataaggtag ttttggagag cccatggccc agcagtactc
     4501 gcagtggtat tttcttgtgg acgaaagtac ggaatgttgt acatggtttg gcccagttta
     4561 ggcaaccact taaaaagact gggcaaaaat cacaggtctt ttcaatatca ggcggtcagt
     4621 ctgaccaggg ctacgggtct aaggatgagc tggtaaagga gggtgcagat ggtcacgccc
     4681 ctgaagagca cacgccacca gagctgacga ccacagagct gcacatcgaa gaggagtgtg
     4741 atatatctgc cattgtctca aaacacctgc agcctactcc tgaacctcag agtcctacgg
     4801 agcctcctgc atggggcagc agtattgtga aagttccatc tgggttattt gataccaaca
     4861 ataggacaag tactggtagt acttcaactg tgctattttc tactcaagct ccagtcgagg
     4921 atgcagtctt cagtgaggtt actaatttca agaagaatgg tgatagagga gagaaaaaac
     4981 agaagcattt ccctgagagg agctgtagct ttagttctga aagtcgagcg gggatgctgc
     5041 ttaagaagag cagtttggac ctgaattcaa gcgaaatggc catcatgatg ggagcagatg
     5101 ccaagatcct cacagcagca ctgacatgtc ctaagacttc cccgcctcat gtcacaagaa
     5161 ctcacagctt tgagaatgtt aactgtcatc tagctgacag taggactcgc atgtcagaag
     5221 gcacccggga ttctgagcac agatcatctc ctgtgctaga gatgctggag gaaagccagg
     5281 agctcctgga gcctgtggtt ggtgataatg tggcagagac tgcggcagag atgacgtgta
     5341 acagtctaca aagtaacagt cacagtgacc agtccagaga cacacaagct ggagcccaag
     5401 atccagtgaa caagaggagc agttcatatg ctactaggaa agccattgag agggaggatg
     5461 tggagacggg actagatccg ttgtctcttt tagccactga atgtgtagaa aaaacgtctg
     5521 attctgaaga taagttattt tctccagtaa tttcacgtaa tctggctgat gaaattgaaa
     5581 gttacatgaa cctaaaaagc ccactaggca gtaaatcttg tagtatggaa ttgcatggag
     5641 aggggaacca ggagcctggc tctcctgctg tgtttgctca ccctttagaa aggagctcga
     5701 gtctgccttc agaccgtggc ccgccagcac gagacagcac cgagactgag aagagctccc
     5761 ctgctgtgtc cagctctaaa actctgactg ggcgtttcaa gccacagagt ccttaccgcg
     5821 cttacaaaga ccggtccact tccttatcag cattagtacg ttcttcacca aacagctctc
     5881 tgggttctgt agtcaattct ctatcggggc taaagttgga caatatactc tcagggccca
     5941 agatagatgt cctaaaatct agtatgaaac aagcagcaac ggtggccagt aagatgtggg
     6001 tggctgtagc atctgcctat agctactcag atgacgagga ggaaactaat aaagattaca
     6061 gtttcccagc tggcctagaa gaccatcata tagtgggaga gactctgtcg cctaacacaa
     6121 gtgtctcagg gttggttccc agtgaactga cccagagcaa cacaagcctc ggcagtagta
     6181 gcagcagtgg agacgttgga aaactgcagt gtccagcagg tgaagttcca ttttcaagaa
     6241 atatcaaagg gcaggacttt gaaaagtcag accatggttc ttctcagaat accagtatgt
     6301 ctagcatcta tcaaaattgt gcgatggagg ttttgatgtc gagttgctca cagtgcagag
     6361 cttgtggcgc tctggtttat gatgaggaaa tcatggctgg atggacagca gatgactcaa
     6421 atttgaatac aacctgcccg ttctgtaaaa gcaacttctt gcctcttctc aatgtagaat
     6481 ttaaagacct gcgaggctct gccagctttt tcttgaaacc aagcacgtct ggtgacagtt
     6541 tacaaagcgg cagcattcca tcagccagtg agccctctga acacaaaccc acgtccagtt
     6601 cagcagaacc tgacctgatc agctttatgg atttctcaaa acacagtgag accataacag
     6661 aagaagcaag ttatacagtc gaatcaagtg atgaaataaa gaaaaccaat ggtgatgtcc
     6721 agagtgtgaa aatgtcatct gtgcctaaca gtttatctaa gcgaaacgta tctttgactc
     6781 gaagtcacag tgttggaggc cctctgcaga atatcgactt ctcccagcga ccatttcatg
     6841 gcgtctcaac agtcagtctt ccaagcagtc tgcaggaaga tgtggaccat ctaggcaagc
     6901 gacccagtcc tcctcctgtg tctgtgccct atttgagtcc tctagtgctt cggaaggaac
     6961 ttgaatcttt gctagaaaat gaaggtgatc aggtcatcca tacatcctca ttcatcaatc
     7021 aacacccaat cattttctgg aacctggttt ggtatttcag acgtttggac cttcctagca
     7081 acttgccagg ccttattctc acctctgagc attgtaatgg aggtgtgcag cttcctctat
     7141 catctttgtc ccaggacagc aaactggtgt atattcaact gctgtgggat aatatcaacc
     7201 ttcatcagga accaggagaa cctctgtatg tctcttggag gaacttgaat tctgagaaga
     7261 agccgtcact tctgtcagag cagcagcagg cagccagcgc tttagtggag acgatcaggc
     7321 agagcatcca gcagaatgac gttctcaagc ccatcaacct gctttcccag caaatgaagc
     7381 caggcacgaa acggcagagg agtttataca gagaaatcct tttcttatca ttagtgtctc
     7441 ttgggagaga aaacattgat attgaggctt ttgacaatga atatggactc gcatacagaa
     7501 gtctgccttc agagagtctt gaaaggttac agagaatcga tgccccaccc agtatcagtg
     7561 tggagtggtg caggaagtgt tttggagcgc ctctcattta agcggccgcg actctagagt
     7621 cgacggatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     7681 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     7741 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     7801 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     7861 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     7921 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     7981 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     8041 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg
     8101 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     8161 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     8221 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggata
     8281 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     8341 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8401 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8461 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     8521 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     8581 gtccaaactc atcaatgtat cttatcatgt ctcgatccgc tgcattaatg aatcggccaa
     8641 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     8701 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8761 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8821 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8881 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8941 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9001 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9061 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9121 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9181 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9241 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     9301 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9361 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9421 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9481 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     9541 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     9601 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     9661 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9721 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9781 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9841 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9901 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9961 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    10021 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    10081 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    10141 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    10201 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
    10261 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    10321 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    10381 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    10441 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    10501 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    10561 aaacaaatag gggttccgcg cacatttccc cgaaaa
//
