LOCUS       sequence-488609-        7318 bp    DNA     circular SYN 07-OCT-2025
DEFINITION  Rab10 GAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Wang J, Nishiyama J, Parra-Bueno P, Okaz E, Oz G, Liu X, Watabe T, 
            Suponitsky-Kroyter I, McGraw TE, Szatmari EM, Yasuda R
  TITLE     Rab10 inactivation promotes AMPAR trafficking and spine enlargement 
            during long-term potentiation.
  JOURNAL   Elife. 2025 Sep 23;13:RP103879. doi: 10.7554/eLife.103879.
  PUBMED    40986440
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2025-09-23"; volume: "13:RP103879. doi"; pages: " 
            10.7554/eLife.103879"
COMMENT     Sequence Label: pCAGGS-3HA-Evi5l
FEATURES             Location/Qualifiers
     source          1..7318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        400..677
                     /label=chicken beta-actin promoter
     intron          678..1698
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1618..1640
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1706..1725
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1768..1777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1780..1806
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1810..1836
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1840..1866
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4350..4369)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4415..4470
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4469..4488)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4831..4847)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4831..4847)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4844..4866)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4855..4871
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4879..4885,4886..4903,4904..4909))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4924..4945
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5004..5199
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5050..5185
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5205..5339
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5242..5261)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5296..5315
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5407..5424)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5578..6166)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5658..5677)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6337..7128,7129..7197))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6960..6979
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7198..7302)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtgccacctg ggtcgatcga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
      421 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
      481 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg
      541 ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg
      601 gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc
      661 gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg
      721 ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc
      781 gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt
      841 ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg
      901 gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc
      961 ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg
     1021 aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag
     1081 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
     1141 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     1201 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     1261 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
     1321 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     1381 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     1441 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     1501 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     1561 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     1621 tcggcttctg gcgtgtgacc ggcggctcta gctagagcct ctgctaacca tgttcatgcc
     1681 ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt
     1741 ggcaaagaat tcgctagcgc taccggtgcc accatggcat acccttatga cgtaccagat
     1801 tacgcgggat atccctacga tgtccccgat tacgcgcgtt atccatatga cgtcccggac
     1861 tatgccgaat tcctcgagag cctgtccacc atggcaagcc ccactctgag ccccgactcc
     1921 tcatctcagg aggccctgtc agcacccacc tgctccccaa cctctgactc cgagaacctc
     1981 agcccagatg agctagaact actggccaag cttgaggagc agaaccggct cctggaagcc
     2041 gactccaagt ccatgcgctc catgaatggc tcccggagga acagcggctc ctccctggta
     2101 tccagctcct ccgcctcctc caacctgagc cacctggagg aggacacatg gattctctgg
     2161 ggccggattg ccaacgagtg ggaggaatgg cggcgcagaa aggaaaagct actcaaggag
     2221 ctgatccgaa agggcatccc acaccacttc cgggccatcg tttggcagct cctgtgcagt
     2281 gccacagata tgcccgtcaa aaaccagtac tccgagctcc tcaagatgtc ctcaccatgt
     2341 gagaagctga tccgaaggga cattgcccgc acctatccag agcatgagtt cttcaaaggc
     2401 caggacagcc tgggccagga agtcctcttc aatgtcatga aggcatactc cctggttgat
     2461 cgggaggttg gatattgcca aggcagtgcc ttcattgtgg gcttactcct catgcagatg
     2521 cctgaggaag aggccttctg tgtgtttgtg cggctgatgc aggagtaccg gctacgggag
     2581 ctcttcaagc ccagcatggc cgagctggga ctctgtatct accagtttga atacatgctg
     2641 caggagcagc ttccggacct gaacacccac ttccgctcac agagcttcca cacatccatg
     2701 tatgcatcat cctggttcct cacgcttttc ctcaccacct ttcccctgcc tgttgctacc
     2761 cgggtctttg acatctttat gtatgaggga ctggagattg tgttccgagt cggccttgcc
     2821 ctgctgcaag tgaaccagac agagctgatg cagctggaca tggagggcat gtcccagtac
     2881 ttccagagag tgatccccca ccagtttgac agctgcccag acaagctggt cctcaaggct
     2941 taccaggtca agtacaaccc taaaaagatg aagaggctgg agaaggagta cgcagctatg
     3001 aagagtaagg agatggagga gcagatcgag atcaaaaggc ttcgaacaga gaaccggctg
     3061 ctcaaacagc ggattgagac cctagagaag gagagcgcgg ccttggctga taggttaatc
     3121 cagggacaag tgacacgggc acaggaggct gaggagaact atgtcatcaa gcgggagttg
     3181 gcagtagtga gacaacaatg tagctcgact gcagaggacc ttcagaaagc acagagtacc
     3241 attcggcagt tgcaagaaca gcaggaaaac cctcgcctaa cagaggactt tgtttcccac
     3301 ctggagacag aactggagca gtcacggtta cgggagaccg agactctggg ggcccttcga
     3361 gaaatgcagg acaaagttct ggacatggaa aagagaaaca gctcactgcc ggatgagaac
     3421 aatgtagcca ggctgcagga agaactcaag gccctcaagg tgcgggaggg cgaggccgta
     3481 gcctctgcgc gggaactgaa gctgcagctg caggagctct cagacacctg gcaggctcat
     3541 gtttcccgcg gtggccgctg gaaggagtcc ccgcggaagc tggtcctggg agagttacag
     3601 gatgagctca tgactatgcg tttgcgtgag gcacaggccc tggcggatgg ccgtgagtgg
     3661 cggcagcgcg tggtggaact ggagacgcag gacaacatcc accgcaacct gctgaaccgg
     3721 gtggaggcgg aacgcgcggc tctgcaggag aagttgcagt acctggcagc acagaacaag
     3781 ggactccaga ctcagctcag cgaaagccgc cgcaagcagg ccgaggctga gtgcaagagt
     3841 aaggaggaag tgatggctgt gcgccttcgg gaggcagaca gcatggctgc agtggcggag
     3901 atgaggcagc gcatcgcaga gctggagatc cagagggagg agggccgcat ccagggccag
     3961 ctgaaccatt cggattcatc gcagtacatc cgtgagctga aggaccagat tgaggagctg
     4021 aagactgagg ttcggttgct gaaaggccca cccacattcg aggacccgct ggctttcgat
     4081 gggctgagcc taacgcggca cctggacgag gattcgctac cgtcttctga tgaggagctg
     4141 cttggtgtgg gtgtgggcgt gggcgtgggc gcagccctgc aggacccact ctaccctctg
     4201 tctccccggg acgcacgctt cttcagaagt ctggaccggc cagccaagga cagcgaaggc
     4261 agctcagaca gcgatgcaga tgagctagcc acgccttaca gcaaccaggt cctggacaac
     4321 tgagcggccg cgactctaga gtcgacggat ctttttccct ctgccaaaaa ttatggggac
     4381 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     4441 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     4501 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     4561 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     4621 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     4681 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     4741 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     4801 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4861 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     4921 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4981 aaacctgtcg tgccagcgga tacgcatctc aattagtcag caaccatagt cccgccccta
     5041 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5101 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5161 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag
     5221 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5281 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctcgatcc
     5341 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     5401 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     5461 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5521 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5581 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5641 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5701 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5761 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5821 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5881 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5941 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     6001 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     6061 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     6121 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     6181 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     6241 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     6301 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     6361 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     6421 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6481 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6541 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6601 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6661 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6721 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6781 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6841 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6901 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6961 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     7021 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     7081 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     7141 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     7201 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7261 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaa
//
