LOCUS       sequence-488614-        7502 bp    DNA     circular SYN 07-OCT-2025
DEFINITION  Rab7 GEF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7502)
  AUTHORS   Wang J, Nishiyama J, Parra-Bueno P, Okaz E, Oz G, Liu X, Watabe T, 
            Suponitsky-Kroyter I, McGraw TE, Szatmari EM, Yasuda R
  TITLE     Rab10 inactivation promotes AMPAR trafficking and spine enlargement 
            during long-term potentiation.
  JOURNAL   Elife. 2025 Sep 23;13:RP103879. doi: 10.7554/eLife.103879.
  PUBMED    40986440
REFERENCE   2  (bases 1 to 7502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2025-09-23"; volume: "13:RP103879. doi"; pages: " 
            10.7554/eLife.103879"
COMMENT     Sequence Label: pCAGGS-3HA-VPS39
FEATURES             Location/Qualifiers
     source          1..7502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        400..678
                     /label=chicken beta-actin promoter
     intron          679..1699
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1619..1641
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1707..1726
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1769..1778
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1781..1807
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1811..1837
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1841..1867
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4534..4553)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4599..4654
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4653..4672)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5015..5031)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5015..5031)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5028..5050)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5039..5055
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5063..5069,5070..5087,5088..5093))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5108..5129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5188..5383
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5234..5369
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5389..5523
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5426..5445)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5480..5499
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5591..5608)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5762..6350)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5842..5861)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6521..7312,7313..7381))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7144..7163
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7382..7486)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtgccacctg ggtcgatcga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
      421 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
      481 aattattttg tgcagcgatg ggggcggggg gggggggggg ggcgcgcgcc aggcggggcg
      541 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc
      601 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
      661 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc
      721 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
      781 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
      841 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
      901 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
      961 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc
     1021 gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa
     1081 ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg
     1141 ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
     1201 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg
     1261 ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc
     1321 ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg
     1381 taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct
     1441 gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg cgccggcagg
     1501 aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc
     1561 cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg cagggcgggg
     1621 ttcggcttct ggcgtgtgac cggcggctct agctagagcc tctgctaacc atgttcatgc
     1681 cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt
     1741 tggcaaagaa ttcgctagcg ctaccggtgc caccatggca tacccttatg acgtaccaga
     1801 ttacgcggga tatccctacg atgtccccga ttacgcgcgt tatccatatg acgtcccgga
     1861 ctatgccgaa ttcctcgaga tgcatgacgc ttttgagccg gtgccgatcc tggagaagct
     1921 tccactgcag attgactgcc tggctgcctg ggaggaatgg cttcttgttg gaaccaagca
     1981 agggcatctt cttctctaca ggattcggaa ggatgttggt tgtaacaggt ttgaggtaac
     2041 actagagaag tccaataaga acttttccaa aaagatccag cagatccatg tggtttccca
     2101 gtttaagatt ctagtcagct tgttagaaaa caacatttat gtccatgact tactgacatt
     2161 tcaacaaatc actacagttt caaaggctaa aggagcatca ctgttcacct gtgacctcca
     2221 gcacactgag acgggtgagg aggtgttgcg gatgtgtgtg gcggtgagga agaagctgca
     2281 gttgtacttc tggaaggata gagaattcca tgaactgcag ggagacttta gtgtacccga
     2341 tgtgcccaag tccatggcat ggtgtgaaaa ttctatctgt gttggcttca agagagacta
     2401 ctacctgatt agggtggatg caaaggggtc tatcaaagag ctcttcccaa caggaaagca
     2461 gctggagccc ttagttgccc ctctggcaga tggaaaagta gccgtgggtc aggatgatct
     2521 gactgtggtg ctcaacgagg aaggcatctg cactcagaaa tgcgccctga actggacaga
     2581 catcccggtg gccatggagc accagcctcc gtacattgtc gcagtgttgc caagatacgt
     2641 ggagatccga acacttgaac caaggcttct ggtccagagc atagagttgc aaaggccccg
     2701 cttcattaca tcagggggat caaacattat ctatgtggcc agcaatcatt ttgtttggag
     2761 actcattcct gtccccatgg caacccaaat ccaacagctt ctccaggaca agcagtttga
     2821 attggctctg cagctagcgg aaatgaaaga tgactcagac agtgaaaagc agcagcaaat
     2881 ccatcacatt aagaacttgt atgccttcaa cctcttctgt cagaagcgct ttgatgagtc
     2941 catgcaggtc tttgctaaac ttggcacaga tcccacccac gtgatgggct tgtaccccga
     3001 cctgctgccc acggactaca ggaagcaact gcagtacccc aacccgctgc ccacactctc
     3061 tggtgctgag ctggagaagg ctcacttagc gctcattgac tacctgacac agaagcggag
     3121 ccagttggta aagaagctga atgactcgga ccaccagtcc agcacctccc cactcatgga
     3181 gggcactccc accatcaagt ccaagcagaa gctgctgcag atcatcgaca ccactctact
     3241 caagtgctac ctccatacaa atgtggccct ggtggctccc ttgctgcgcc tggagaacaa
     3301 ccactgtcac atcgaggaga gtgagcatgt gctgaagaag gctcacaagt acagtgagct
     3361 gatcatcctg tatgagaaga aggggctcca tgagaaagct ctgcaggtgc tagtggacca
     3421 gtccaagaaa gcaaactcac ctctgaaagg ccatgaaagg acggtgcagt acctacagca
     3481 tttgggcaca gaaaacctac atttgatttt ttcctactcg atctgggtgc tgagagactt
     3541 cccagaagat ggcctgaaga tattcactga agacctcccg gaggtggagt ctctgccacg
     3601 agatcgcgtg ctcaacttct tgatagagaa tttcaaggcc ctggccattc cttacctgga
     3661 gcacatcatc cacgtgtggg aggagacagg cacacggttc cacaattgcc taatccagct
     3721 gtactgtgag aaagtgcaga atctgatgaa agactacctc ctgtcactac ccacaggcaa
     3781 aagtccagtc cctgctggcg aggaagcagg agaactggga gaatcccgac agaagctgct
     3841 cacgttcctg gagatttcca gctcctacga tcccggccgg ctcatctgtg attttccctt
     3901 cgatggcctc ttagaagaaa gagctctcct gttgggacgc atggggaagc acgaacaagc
     3961 tctcttcatc tatgtccatg tcttgaagga cacaaagatg gctaaggagt actgccacaa
     4021 acactacgac caaaacaaag aaggcaacaa agatgtatat ctgtccctgc ttcggatgta
     4081 cctgtcgcct ccaagcattc actgcctggg gccaatcaaa ctagagctcc tggagccaca
     4141 ggccaacctc caggctgccc tgcaggtcct agagctgcac tacagcaaat tagacaccac
     4201 caaggcaatc aaccttctgc cagcaaacac tcagatcaat gacatacgaa tcttcctaga
     4261 aaaggtctta gaagaaaatg cacaaaagaa acggttcaat caagtgctta agaatcttct
     4321 ccatgcggag tttctcaggg ttcaggaaga gcggatttta caccagcagg tgaagtgcat
     4381 catcactgag gagaaggtgt gcatggtgtg caagaagaag attgggaaca gtgcatttgc
     4441 aagatacccc aatggagtgg ttgtgcacta cttctgctcc aaagaagtta actcagccga
     4501 cacctgagcg gccgcgactc tagagtcgac ggatcttttt ccctctgcca aaaattatgg
     4561 ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat
     4621 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     4681 tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct
     4741 ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc
     4801 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     4861 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     4921 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc
     4981 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5041 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     5101 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     5161 cgggaaacct gtcgtgccag cggatacgca tctcaattag tcagcaacca tagtcccgcc
     5221 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5281 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5341 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt
     5401 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5461 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctcg
     5521 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     5581 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     5641 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     5701 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     5761 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     5821 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     5881 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     5941 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6001 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6061 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6121 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6181 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6241 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     6301 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     6361 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     6421 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6481 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6541 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6601 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6661 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6721 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     6781 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     6841 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     6901 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     6961 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7021 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7081 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7141 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7201 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7261 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7321 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7381 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     7441 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     7501 aa
//
