LOCUS       sequence-488625-        7149 bp    DNA     circular SYN 07-OCT-2025
DEFINITION  Rab5 GEF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Wang J, Nishiyama J, Parra-Bueno P, Okaz E, Oz G, Liu X, Watabe T, 
            Suponitsky-Kroyter I, McGraw TE, Szatmari EM, Yasuda R
  TITLE     Rab10 inactivation promotes AMPAR trafficking and spine enlargement 
            during long-term potentiation.
  JOURNAL   Elife. 2025 Sep 23;13:RP103879. doi: 10.7554/eLife.103879.
  PUBMED    40986440
REFERENCE   2  (bases 1 to 7149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2025-09-23"; volume: "13:RP103879. doi"; pages: " 
            10.7554/eLife.103879"
COMMENT     Sequence Label: pCAGGS-RIN1
FEATURES             Location/Qualifiers
     source          1..7149
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        400..676
                     /label=chicken beta-actin promoter
     intron          677..1697
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1617..1639
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1705..1724
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(4181..4200)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4246..4301
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4300..4319)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4662..4678)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4662..4678)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4675..4697)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4686..4702
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4710..4716,4717..4734,4735..4740))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4755..4776
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4835..5030
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      4881..5016
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5036..5170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5073..5092)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5127..5146
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5238..5255)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5409..5997)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5489..5508)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6168..6959,6960..7028))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6791..6810
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7029..7133)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtgccacctg ggtcgatcga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
      421 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
      481 aattattttg tgcagcgatg ggggcggggg gggggggggg cgcgcgccag gcggggcggg
      541 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
      601 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
      661 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
      721 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
      781 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
      841 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
      901 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
      961 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     1021 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     1081 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     1141 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     1201 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
     1261 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
     1321 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
     1381 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     1441 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     1501 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     1561 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     1621 cggcttctgg cgtgtgaccg gcggctctag ctagagcctc tgctaaccat gttcatgcct
     1681 tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg
     1741 gcaaagaatt cgccaccatg tgcacgcctg tgccttgggg gttggattct cttcctgagt
     1801 cccaggagaa gctgaaggag ctcccagcca tggaagaccc tggtgagacc gaagcacacc
     1861 ctctgggagc caccagcctg aactttgtac ctgggtacca acaggaagaa aagccatctc
     1921 cagacccact gtatgacaca cctgatgcca gaggggttca ggcaggcggg tcccaacaac
     1981 cagcacgcac agtgagcttg cgggagcggc tgctgatcac ccggcctgtg tggttacagc
     2041 tgcgggccaa cgctgcagcc gcactgcacg tgctgaggac agagcctccg gggaccttcc
     2101 tggtgcggaa atctaacact cgccagtgcc agactctatg tgtgcggctg ccagaagcta
     2161 gtggcccctc ttttgtctcc agccactatc tccaagagag tcctggcggc atctctttgg
     2221 agggctcaga gctcacattc ccggacctgg tccagctcat ctgtgcatac tgtcatacca
     2281 gggacattct tctcctccct ctccgactcc ccagagccat tcatcaggca gccacccaca
     2341 aagagctgga ggccatctcc catctgggca tggagttctg gagttcctct ctcaacacca
     2401 agaaccaaca gagaccttca gaagctccgc agataccccg gctgaaggct cgctcccctc
     2461 aagagctgga tcagggcact ggtgcagccc tctgcttctt caaccccctc ttccccgggg
     2521 atctgggccc caccaaacgt gagaaattca agaggagttt caaagtgcgt gtatccacag
     2581 agacctccag ccctctgtct ccacctgctg tgccgcctcc tccagtcccc gttctgccag
     2641 ggacatcttc cagccaaacg gaaagactgc cccctcgaca gctgctgcag agggagagct
     2701 cagtgggata ccgtgtgcca ggaagtgctt ctggtcctag tcttcctccc ctaccttcac
     2761 ttcaggaggt ggactgctgc tcccccagca gctcagagga ggagggttct tcaggaagcc
     2821 ctacaacctc cccacgccta agccgcccaa ggcaccggcg gcctctgctt cggtccatga
     2881 gttctgcttt ctgctctctg ctggcaccag agaggcaggt gggccgggca gctacaatgc
     2941 taatgcaaaa ccgatacaca gctgtgggtc agctagtgca ggacctattg acccaggttc
     3001 gtgttggtcc tgagtcccgg gagttgcagg gcatccgaca ggccctgagt cgggcacggg
     3061 ccatgctgag tgcagagctg ggccctgaga aactgctacc accagagcgg ctggaattgg
     3121 tcctggagaa atccctgcat cgctctgttc tcaagcctct tcgacccatt ctagctgccc
     3181 gcctgcggcg ccgactttcc acagacggtt cccttggccg cctagctgag ggcttccgcc
     3241 tggcccggac ccaggggcct ggagccttcg ggtcccacct gaacctttct tccccagtgg
     3301 agatagaacc agtgcgtcag aagctgctgc agctccttcg tgcctactcg cctagtgccc
     3361 agatcaagtg gctcctgcag gcctgcaagt tgctatacac agccctgaaa acccaggcag
     3421 gggagaatgc aggtgctgat gagttcctgc ctctcttgag ccttgtcttg gcccagtgcg
     3481 accttcctga cctcctgtta gaagctgagt atatgtcaga gctactggaa cccacattgc
     3541 tcactgggga gggcggctac tacctgacca gcctctcagc cagcctggcc ttgttgagtg
     3601 gcctaagcca ggcccacgca ctgccactca gccctgccca ggagctccaa cgttctctgg
     3661 ccctctggga acagcgccgc ctgccggcca cccacaactt ccagcacctc ctccgagtag
     3721 cctaccagga ccccagcaca ggttgcacct ccaagacctt ggctgtgccc ccggggtctt
     3781 cgattgccac cctgagtcaa ctctgtgcca ccaagttccg agtgacccaa ccggatgcat
     3841 ttggcctctt tctctacaag gaccagggct accatcgcct gccccctgaa gccctagtcc
     3901 acaggcttcc cacaactggt tacctcatct accgccgggc agagcgcccc gagacccaga
     3961 gggctgcaac agagaagacc aagacaggaa atgagaggcc agaggcagga gcatgggagg
     4021 aggagaaagg gggtctaaat ggtgaaggga aatctgagat agctgttgac caagaaggca
     4081 aggatcaggc cagaggaggt cacatgcagc tagaggaaca aaaggcagag ggatgcccgg
     4141 ctttagaaga gtaggcggcc gcgactctag agtcgacgga tctttttccc tctgccaaaa
     4201 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     4261 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     4321 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     4381 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     4441 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     4501 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     4561 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     4621 ggagatccct cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     4681 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4741 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4801 ttccagtcgg gaaacctgtc gtgccagcgg atacgcatct caattagtca gcaaccatag
     4861 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     4921 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     4981 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctaactt
     5041 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5101 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5161 tgtctcgatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5221 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5281 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5341 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5401 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5461 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5521 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5581 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5641 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5701 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5761 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     5821 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     5881 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     5941 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6001 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6061 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6121 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6181 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6241 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6301 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6361 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6421 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6481 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6541 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6601 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6661 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6721 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6781 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     6841 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     6901 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6961 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7021 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7081 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7141 ccccgaaaa
//
