LOCUS       sequence-488640-       10241 bp    DNA     circular SYN 07-OCT-2025
DEFINITION  Expresses Dynein-1 LIC2 as a mGFP fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10241)
  TITLE     pLVXPuro-mGFP-LIC1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVXPuro-mGFP-LIC2
FEATURES             Location/Qualifiers
     source          1..10241
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2828..2837
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2834..2836,2837..2839,2840..3550)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2879..2900)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3140..3159)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3487..3508
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        5076..5575
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5111..5134)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5508..5527
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5596..6195
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5596..5615)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6092..6112
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6209..6797
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6262..6282)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6680..6691)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7004..7637
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7766..7782)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7766..7782)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7779..7801)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7790..7806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7814..7820,7821..7838,7839..7844))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7859..7880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7997..8014)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8168..8756)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8248..8267)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8927..9718,9719..9787))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9550..9569
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9788..9892)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9940..10074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9977..9996)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10031..10050
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgac
     2821 accggtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     2881 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     2941 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     3001 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     3061 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     3121 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     3181 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     3241 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     3301 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     3361 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     3421 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
     3481 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     3541 gctgtacaag ctcgaggcag ctgcagctat ggcgccggtg ggggtggaga agaagctgct
     3601 gctaggtccc aacgggcccg cggtggcggc cgccggcgac ctgaccagtg aggaggagga
     3661 aggccagagc ctatggtcct ccattctgag cgaagtgtcc acccgcgcca ggtccaagct
     3721 gccgtccggc aagaacatcc tggtcttcgg tgaagatggt tctggtaaaa caaccctcat
     3781 gactaaacta caaggagctg agcatggcaa aaaaggaaga ggcctagaat atctctacct
     3841 cagtgtccat gatgaggacc gagatgatca cacgcgctgc aacgtgtgga ttctggatgg
     3901 agacttgtac cacaaaggcc tgctgaaatt tgcagtttct gctgaatcct tgccagagac
     3961 cctcgtcatt tttgttgcag acatgtctag accttggact gtgatggaat ctctgcagaa
     4021 atgggctagt gttttacgtg agcacattga taaaatgaaa attccaccag aaaaaatgag
     4081 ggagctggaa cggaagtttg tgaaagattt tcaagactat atggaacctg aagaaggttg
     4141 tcaaggttcc ccacagagaa gaggccctct gacctcaggc tccgatgaag aaaatgttgc
     4201 cctgcctctg ggtgacaatg tgctgactca taacctgggg atcccggtgt tggtggtgtg
     4261 cacaaagtgt gatgcggtga gtgtcctgga gaaggagcac gattacaggg atgagcattt
     4321 ggactttatc cagtcacacc tgcggaggtt ctgccttcag tatggagctg ccttgattta
     4381 cacatcagtg aaagaagaga aaaacctcga cttgttgtat aagtatattg ttcataaaac
     4441 atacggtttc cacttcacca cacctgcctt agttgtggaa aaggatgccg tttttatacc
     4501 tgcaggctgg gacaatgaaa agaaaatagc tattttacat gaaaatttta caaccgtgaa
     4561 gccggaagat gcatatgaag actttattgt gaaacctccc gtgagaaagc tggtccacga
     4621 caaagagttg gcagcagaag atgagcaggt gttcctaatg aagcaacagt cactccttgc
     4681 caagcaacca gccactccca cgagagcttc tgaatctcct gcaagaggac cctctggctc
     4741 tccaaggacc cagggtcggg gagggccagc cagtgtgcct agctcctccc caggcacgtc
     4801 agtaaaaaag ccagacccaa acatcaaaaa taatgcagca agtgaagggg tgttggccag
     4861 cttcttcaac agtctgttga gtaaaaagac aggctctcct ggaagtcctg gtgctggtgg
     4921 ggtgcagagc acagccaaga actcaggaca aaagactgtg ttgtcaaatg ttcaggaaga
     4981 actggataga atgactcgaa agccagactc tatggtaaca aactcttcaa cagaaaatga
     5041 agcctgaaga tcccgcgact ctagataatt ctaccgggta ggggaggcgc ttttcccaag
     5101 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
     5161 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
     5221 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
     5281 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
     5341 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
     5401 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
     5461 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     5521 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     5581 cagcccaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     5641 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     5701 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     5761 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     5821 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     5881 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     5941 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6001 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6061 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6121 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6181 aagcccggtg cctgaccgcg tctggaacaa tcaacctctg gattacaaaa tttgtgaaag
     6241 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6301 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6361 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6421 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6481 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6541 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6601 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6661 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6721 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6781 ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa acatggagca
     6841 atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag
     6901 gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag
     6961 gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg gctaattcac
     7021 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     7081 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
     7141 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
     7201 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
     7261 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
     7321 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
     7381 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
     7441 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     7501 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     7561 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     7621 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     7681 aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttta ccgtcgacct
     7741 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7801 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7861 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7921 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7981 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8041 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8101 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8161 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8221 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8281 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8341 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8401 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8461 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8521 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8581 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8641 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8701 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8761 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8821 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8881 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8941 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9001 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9061 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9121 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9181 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9241 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9301 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9361 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9421 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9481 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9541 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9601 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9661 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9721 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9781 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9841 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9901 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat
     9961 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    10021 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    10081 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    10141 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    10201 agaaattgta tttgttaaat atgtactaca aacttagtag t
//
