LOCUS       sequence-488660-       10219 bp    DNA     circular SYN 08-OCT-2025
DEFINITION  Express HA N-terminal tagged mouse Galphaolf transiently via 
            transfection or stably via lentivirus-mediated infection in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Xun Y, Jiang Y, Xu B, Tang M, Ludwig S, Nakamura K, Mukhopadhyay S, 
            Liu C, Beutler B, Zhang Z
  TITLE     GPR45 modulates Galpha(s) at primary cilia of the paraventricular 
            hypothalamus to control food intake.
  JOURNAL   Science. 2025 Jun 5;388(6751):eadp3989. doi: 
            10.1126/science.adp3989. Epub 2025 Jun 5.
  PUBMED    40472089
REFERENCE   2  (bases 1 to 10219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-06-5"; volume: "388(6751):eadp3989. doi"; pages: " 
            10.1126/science.adp3989. Epub 2025 Jun 5"
COMMENT     Sequence Label: pBOB-HA-Gnal
FEATURES             Location/Qualifiers
     source          1..10219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3142..3168
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          4620..4849
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4832..4851
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5026..5614
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5079..5099)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5497..5508)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6021..6201
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6227..6244)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6233..6457
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6503..6931
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6590..6609)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6800..6821
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6940..6960)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6945..7274
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7125..7260
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7187..7206
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7322..7369
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7388..7762
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7892..8013
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7929..7948)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7983..8002
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8062..8078)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8062..8078)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8075..8097)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8086..8102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8110..8116,8117..8134,8135..8140))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8155..8176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8293..8310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8464..9052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8544..8563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9223..10014,10015..10083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9846..9865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10084..10188)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagccaccat gtacccatac gatgttccag attacgcttc tagagaattc
     3181 ggatccatgg gcctatgcta cagcctgcgg ccgctgctct tcgggagccc agaggacacc
     3241 ccgtgtgcgg cctcggaacc ctgcgcagag gatgctcagc ccagcgccgc cccggcccct
     3301 gcctcgatcc cagccccggc tcccgtaggg accctgctcc ggcgtggcgg cggccggatc
     3361 gtcgcgaacg cgcggccgcc aggcgagctg cagagccgcc ggcgacagga gcagctacga
     3421 gccgaggagc gcgaggcggc taaagaggcg aggaaagtca gccggggcat cgaccgcatg
     3481 ctgcgcgagc agaagcggga cctgcagcag acgcaccggc tcctgctgct gggggctggt
     3541 gagtccggga aaagcactat cgtcaaacag atgaggatcc tgcacgtcaa tggcttcaac
     3601 cccgaggaaa agaagcagaa aattctggac atcaggaaaa atgtcaaaga tgcgatcgtg
     3661 acaatcgttt cagcaatgag tactatcata cctccagttc cactggccaa ccctgagaac
     3721 cagttccggt cagattatat caagagcata gcccctatca ctgactttga atattcccag
     3781 gagttctttg accatgtgaa gaagctgtgg gacgatgaag gagtgaaggc ctgctttgag
     3841 agatccaacg agtaccagct gatcgactgt gcacaatact tcctggaaag gattgacagt
     3901 gtcagtctgg ttgactacac acccacagac caggacctgc tcagatgcag agtgctgaca
     3961 tcaggaatct ttgagacacg attccaagtg gacaaagtga actttcacat gtttgatgtt
     4021 ggaggccaga gagatgagag aagaaaatgg atccagtgtt ttaatgatgt cactgcgatc
     4081 atttacgtgg cggcctgtag tagctacaac atggtgatcc gggaagataa caataccaac
     4141 agacttcggg aatcactgga cctgtttgaa agcatctgga ataacaggtg gttgcgaacc
     4201 atttctatca tcctattctt gaacaaacaa gacatgctgg cagaaaaagt cttggcaggg
     4261 aagtcaaaaa tcgaagacta tttcccggag tatgccaatt atactgtccc tgaagatgca
     4321 acaccagatg cgggagaaga tcccaaagtt acaagagcaa agttctttat ccgggatctg
     4381 ttcttgagga tcagcacagc cacgggtgat ggcaaacatt actgctaccc tcacttcacc
     4441 tgcgccgtgg acacagagaa catccgcaga gtgttcaacg attgccgtga catcatccag
     4501 agaatgcatc tcaagcagta cgaactcttg tgactcgagc catggaagct tgatatctaa
     4561 ctgactgaac cggtggtacc gaggaattaa ttcgctgtct gcgagggcca gctgttgggg
     4621 tgagtactcc ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa
     4681 cgaggaggat ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat
     4741 ctggtcagaa aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg
     4801 gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc
     4861 caggtccaac tgcaggtcga gcatgcatct agggcggcca attccgccga tcctggaaaa
     4921 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     4981 agcacaagag gaggaggagg tgggttttcc agtcacacct cagacaatca acctctggat
     5041 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     5101 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     5161 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     5221 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     5281 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     5341 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     5401 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     5461 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     5521 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     5581 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa
     5641 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     5701 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     5761 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     5821 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     5881 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     5941 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     6001 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6061 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6121 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6181 cagtgtggaa aatctctagc agggccgctt taaacccgct gatcagcctc gactgtgcct
     6241 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     6301 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     6361 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     6421 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     6481 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6541 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6601 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6661 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6721 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6781 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6841 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6901 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     6961 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7021 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7081 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7141 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7201 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7261 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     7321 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     7381 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     7441 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     7501 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     7561 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     7621 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     7681 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     7741 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     7801 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     7861 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     7921 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     7981 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     8041 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8101 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8161 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8221 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8281 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8341 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8401 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8461 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8521 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8581 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8641 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8701 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8761 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8821 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8881 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     8941 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9001 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9061 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9121 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9181 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9241 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9301 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9361 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9421 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9481 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9541 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9601 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9661 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9721 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9781 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9841 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9901 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9961 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10021 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10081 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10141 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10201 aaaagtgcca cctgacgtc
//
