LOCUS       sequence-488668-       10554 bp    DNA     circular SYN 08-OCT-2025
DEFINITION  Stimultaneously express HA C-terminal tagged mouse MC4R and untagged
            MRAP2 protein using a P2A linker, transiently via transfection or 
            stably via lentivirus-mediated infection in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10554)
  AUTHORS   Xun Y, Jiang Y, Xu B, Tang M, Ludwig S, Nakamura K, Mukhopadhyay S, 
            Liu C, Beutler B, Zhang Z
  TITLE     GPR45 modulates Galpha(s) at primary cilia of the paraventricular 
            hypothalamus to control food intake.
  JOURNAL   Science. 2025 Jun 5;388(6751):eadp3989. doi: 
            10.1126/science.adp3989. Epub 2025 Jun 5.
  PUBMED    40472089
REFERENCE   2  (bases 1 to 10554)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-06-5"; volume: "388(6751):eadp3989. doi"; pages: " 
            10.1126/science.adp3989. Epub 2025 Jun 5"
COMMENT     Sequence Label: pBOB-Mrap2-P2A-Mc4r-HA
FEATURES             Location/Qualifiers
     source          1..10554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3145..3154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3781..3837
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4840..4866
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          4955..5184
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5167..5186
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5361..5949
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5414..5434)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5832..5843)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6356..6536
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6562..6579)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6568..6792
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6838..7266
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6925..6944)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7135..7156
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7275..7295)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7280..7609
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7460..7595
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7522..7541
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7657..7704
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7723..8097
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8227..8348
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8264..8283)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8318..8337
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8397..8413)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8397..8413)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8410..8432)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8421..8437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8445..8451,8452..8469,8470..8475))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8490..8511
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8628..8645)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8799..9387)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8879..8898)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9558..10349,10350..10418))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10181..10200
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10419..10523)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagaattcgg atccgccacc atggagatgt ctgcccagag gctggcttct
     3181 aacaggacat ccccacagtc accatcgaac tctgactaca cctgggaata cgagtactat
     3241 gagatcggac cagtttcctt tgaaggactg aaggctcata aatattccat tgtgattgga
     3301 ttctgggttg gtcttgcggt ctttgtgatt ttcatgtttt ttgtgctgac tttgctgacg
     3361 aagacaggtg ccccacacca agacaacgcg gagtcctcag agaggaggtt ccgcatgaac
     3421 agctttgtgt cagacttcgg gaagccgctg gagtcagaca aggtgttttc tcgtcagggc
     3481 aacgaggagt ccaggtctct gttccactgt tacatcaatg aagtagaaca cttggacagg
     3541 gttaaagttt gccaccaaac aacagccatc gacagtgatg tccacctcca ggaagccagc
     3601 agaagcagtg ggaggcctga ggaggagcta gccaggttca tgaagtttga catccccaac
     3661 tttgtgaaca cagagcagag ctcctttggg gaggatgatc tcctgatttc ggaagcaccc
     3721 gttcttctag aaaacaagcc agtttcccag acatcacgca tagacctgga cggcagcggc
     3781 gccaccaact tcagcctgct gaagcaggcc ggcgacgtgg aggagaaccc cggccccatg
     3841 aactccaccc accaccatgg catgtatact tccctccacc tctggaaccg cagcagctac
     3901 gggctgcacg gcaatgccag cgagtcgctg gggaagggcc acccggacgg aggatgctat
     3961 gagcaacttt ttgtttcccc cgaggtgttt gtgactctgg gtgtcataag cctgttggag
     4021 aacattctag tgatcgtggc gatagccaag aacaagaacc tgcactcacc catgtacttt
     4081 ttcatctgta gcctggctgt ggcagatatg ctggtgagcg tttcgaatgg gtcggaaacc
     4141 atcgtcatta ccctgttaaa cagtacggat acggatgccc agagcttcac cgtgaacatt
     4201 gataatgtca ttgactctgt gatctgtagc tccttgctcg catccatttg cagcctgctt
     4261 tccattgcgg tggacaggta tttcactatc ttttacgcgc tccagtacca taacatcatg
     4321 acggttaggc gggtcgggat catcataagt tgtatctggg cagcttgcac tgtgtcaggc
     4381 gtcctcttca tcatttactc ggacagcagc gctgtcatca tctgcctcat ttccatgttc
     4441 ttcactatgc tagttctcat ggcctctctc tatgtccaca tgttcctgat ggcgaggctt
     4501 cacattaaga ggattgctgt cctcccaggc acagggacca tccgccaggg taccaacatg
     4561 aagggggcga ttaccttgac catcctgatt ggagtctttg ttgtctgctg ggccccgttc
     4621 tttctccatt tactgttcta catctcttgc cctcagaatc catactgcgt gtgcttcatg
     4681 tctcatttta atttgtatct catactgatc atgtgtaacg ccgtcatcga ccctctcatt
     4741 tatgccctcc ggagtcaaga actgaggaaa actttcaaag agatcatctg tttctatcct
     4801 ctgggaggca tctgtgagtt gtctagcagg tatctcgagt acccatacga tgttccagat
     4861 tacgcttaat cgagccatgg aagcttgata tctaactgac tgaaccggtg gtaccgagga
     4921 attaattcgc tgtctgcgag ggccagctgt tggggtgagt actccctctc aaaagcgggc
     4981 atgacttctg cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg
     5041 cccgcggtga tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg
     5101 ttgtcaagct tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca
     5161 tccactttgc ctttctctcc acaggtgtcc actcccaggt ccaactgcag gtcgagcatg
     5221 catctagggc ggccaattcc gccgatcctg gaaaaacatg gagcaatcac aagtagcaat
     5281 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     5341 tttccagtca cacctcagac aatcaacctc tggattacaa aatttgtgaa agattgactg
     5401 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     5461 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     5521 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     5581 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     5641 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5701 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     5761 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5821 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5881 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5941 cctccccgcc tggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
     6001 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6061 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     6121 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     6181 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     6241 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     6301 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     6361 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     6421 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     6481 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     6541 cgctttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6601 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6661 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6721 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     6781 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     6841 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6901 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6961 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7021 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7081 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7141 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7201 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7261 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7321 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7381 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     7441 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7501 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     7561 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     7621 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     7681 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     7741 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     7801 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     7861 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     7921 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     7981 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     8041 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     8101 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8161 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8221 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8281 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8341 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     8401 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     8461 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     8521 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8581 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8641 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8701 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8761 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8821 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8881 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8941 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9001 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9061 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9121 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9181 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9241 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9301 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9361 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     9421 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     9481 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     9541 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9601 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9661 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9721 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9781 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9841 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9901 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9961 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10021 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10081 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10141 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10201 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10261 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10321 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10381 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    10441 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    10501 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
