LOCUS       sequence-488693-        9993 bp    DNA     circular SYN 08-OCT-2025
DEFINITION  Express 3xFlag C-terminal tagged mouse GPR45-Del2 mutant transiently
            via transfection or stably via lentivirus-mediated infection in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Xun Y, Jiang Y, Xu B, Tang M, Ludwig S, Nakamura K, Mukhopadhyay S, 
            Liu C, Beutler B, Zhang Z
  TITLE     GPR45 modulates Galpha(s) at primary cilia of the paraventricular 
            hypothalamus to control food intake.
  JOURNAL   Science. 2025 Jun 5;388(6751):eadp3989. doi: 
            10.1126/science.adp3989. Epub 2025 Jun 5.
  PUBMED    40472089
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-06-5"; volume: "388(6751):eadp3989. doi"; pages: " 
            10.1126/science.adp3989. Epub 2025 Jun 5"
COMMENT     Sequence Label: pBOB-Gpr45-Del2-3xFlag
FEATURES             Location/Qualifiers
     source          1..9993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3145..3154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4237..4302
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          4394..4623
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4606..4625
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4800..5388
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4853..4873)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5271..5282)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5795..5975
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6001..6018)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6007..6231
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6277..6705
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6364..6383)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6574..6595
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6714..6734)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6719..7048
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6899..7034
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6961..6980
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7096..7143
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7162..7536
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7666..7787
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7703..7722)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7757..7776
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7836..7852)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7836..7852)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7849..7871)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7860..7876
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7884..7890,7891..7908,7909..7914))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7929..7950
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8067..8084)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8238..8826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8318..8337)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8997..9788,9789..9857))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9620..9639
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9858..9962)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagaattcgg atccgccacc atggcctgta acagcacacc catggggact
     3181 tacgaacatc tgctgctgaa tgtgagcaac actttggacc ctggggacac cccactgtct
     3241 gcaccgctca ggatctcgct ggcaataatg atgctgctga tgatcgtggt aggattcctt
     3301 ggcaacacgg tggtctgcat catcgtgtac cagaggccag ccatgcgttc agctatcaac
     3361 ctgctgctgg ccaccttggc cttctccgac atcatgctgt ctttatgctg catgccattc
     3421 acggccatca ccctcatcac tgttcgctgg catttcgggg accacttttg tcggctttca
     3481 gctactctct attggttttt tgtcctagag ggcgtggcca tcctgctcat cattagcgtg
     3541 gaccggtttc tcatcatcgt gcagcgtcag gacaagctga acccacgcag ggctaagatg
     3601 atcatcgcgg cctcctgggt gctgtctttc tgcatctctg cgccctcctt cactggctgg
     3661 acgttcatgg aggtgcctgc tcgagcccca cagtgcgtgc taggctacac tgagttccca
     3721 gctgaacgcg cctatgtagt gacactggtg gtggcagtgt tctttgctcc cttcggcgtc
     3781 atgttgtgct cctatctgtg catcctcaat acggtgcgga agaacgctgt ccgtgtgcac
     3841 aaccagtcgg acagcctgga cctcagacag ctgaccggag ctggcctgct gagtttcaaa
     3901 accaaggcct tcaccaccat cctcatcctc ttcgtgggct tttcactctg ctggctgcca
     3961 cactcagtct acagcctgct gtctgcgttc agccggcggt tctattacag cgcctccttc
     4021 tacaccacca gcacatgcgt cctgtggctc agttacctca agtctgtttt caaccccatc
     4081 gtctactgct ggaggatcaa gaaattccgc gaggcctgca tagagttgct tccccacact
     4141 ttccaaatcc tccctaaagt gcctgagcgg atccagagga aaatccagcc aagcaccatc
     4201 tatgtgtgca acgaaaacca atccgctgtc ctcgaggact acaaagacca tgacggtgat
     4261 tataaagatc atgacatcga ctacaaggat gacgatgaca agtagtgatc gagccatgga
     4321 agcttgatat ctaactgact gaaccggtgg taccgaggaa ttaattcgct gtctgcgagg
     4381 gccagctgtt ggggtgagta ctccctctca aaagcgggca tgacttctgc gctaagattg
     4441 tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgcggtgat gcctttgagg
     4501 gtggccgcgt ccatctggtc agaaaagaca atctttttgt tgtcaagctt gaggtgtggc
     4561 aggcttgaga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
     4621 caggtgtcca ctcccaggtc caactgcagg tcgagcatgc atctagggcg gccaattccg
     4681 ccgatcctgg aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     4741 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcagaca
     4801 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     4861 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     4921 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     4981 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5041 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5101 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     5161 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     5221 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     5281 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     5341 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattcgag
     5401 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5461 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     5521 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     5581 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     5641 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     5701 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     5761 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     5821 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5881 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5941 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc gctttaaacc cgctgatcag
     6001 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6061 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6121 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     6181 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     6241 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     6301 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6361 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6421 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6481 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6541 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6601 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6661 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     6721 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6781 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6841 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6901 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6961 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     7021 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     7081 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     7141 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     7201 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     7261 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     7321 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     7381 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     7441 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     7501 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     7561 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     7621 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     7681 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7741 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     7801 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     7861 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     7921 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     7981 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8041 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8101 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8161 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8221 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8281 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8341 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8401 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8461 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8521 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8581 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8641 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8701 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8761 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8821 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8881 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8941 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9001 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9061 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9121 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9181 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9241 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9301 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9361 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9421 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9481 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9541 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9601 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9661 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9721 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9781 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9841 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9901 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9961 cgcacatttc cccgaaaagt gccacctgac gtc
//
