LOCUS       sequence-488698-        9952 bp    DNA     circular SYN 08-OCT-2025
DEFINITION  Express HA N-terminal tagged mouse Galphaq transiently via 
            transfection or stably via lentivirus-mediated infection in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9952)
  AUTHORS   Xun Y, Jiang Y, Xu B, Tang M, Ludwig S, Nakamura K, Mukhopadhyay S, 
            Liu C, Beutler B, Zhang Z
  TITLE     GPR45 modulates Galpha(s) at primary cilia of the paraventricular 
            hypothalamus to control food intake.
  JOURNAL   Science. 2025 Jun 5;388(6751):eadp3989. doi: 
            10.1126/science.adp3989. Epub 2025 Jun 5.
  PUBMED    40472089
REFERENCE   2  (bases 1 to 9952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-06-5"; volume: "388(6751):eadp3989. doi"; pages: " 
            10.1126/science.adp3989. Epub 2025 Jun 5"
COMMENT     Sequence Label: pBOB-HA-Gnaq
FEATURES             Location/Qualifiers
     source          1..9952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3142..3168
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          4353..4582
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4565..4584
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4759..5347
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4812..4832)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5230..5241)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5754..5934
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5960..5977)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5966..6190
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6236..6664
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6323..6342)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6533..6554
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6673..6693)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6678..7007
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6858..6993
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6920..6939
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7055..7102
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7121..7495
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7625..7746
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7662..7681)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7716..7735
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7795..7811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7795..7811)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7808..7830)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7819..7835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7843..7849,7850..7867,7868..7873))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7888..7909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8026..8043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8197..8785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8277..8296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8956..9747,9748..9816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9579..9598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9817..9921)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagccaccat gtacccatac gatgttccag attacgcttc tagagaattc
     3181 ggatccatga ctctggagtc catcatggcg tgctgcctga gcgaggaggc caaggaagcc
     3241 cggaggatca acgacgagat cgagcggcag ctgcgcaggg acaagcgcga cgcccgccgg
     3301 gagctcaagc tgctgctgct ggggacaggg gagagtggca agagcacctt catcaagcag
     3361 atgaggatca tccacgggtc gggctactct gacgaagaca agcgcggctt caccaagctg
     3421 gtgtatcaga acatcttcac ggccatgcag gccatgatca gagcgatgga cacgctcaag
     3481 atcccataca agtatgaaca caataaggct catgcacaat tggttcgaga ggttgatgtg
     3541 gagaaggtgt ctgcttttga gaatccatat gtagatgcaa taaagagctt gtggaatgat
     3601 cctggaatcc aggagtgcta cgacagacga cgggaatatc agttatctga ctctaccaaa
     3661 tactatctga atgacttgga ccgtgtagcc gacccttcct atctgcctac acaacaagac
     3721 gtgcttagag ttcgagtccc cactacaggg atcatcgaat acccctttga cttacaaagt
     3781 gtcattttca gaatggtcga tgtagggggc caaaggtcag agagaagaaa atggatacac
     3841 tgctttgaaa atgtcacctc catcatgttt ctagtagcgc ttagcgaata tgatcaagtt
     3901 cttgtggagt cagacaatga gaaccgcatg gaggagagca aagcactctt tagaacaatt
     3961 atcacctacc cctggttcca gaactcctct gtgattctgt tcttaaacaa gaaagatctt
     4021 ctagaggaga aaatcatgta ttcccaccta gtcgactact tcccagaata tgatggaccc
     4081 cagagagatg cccaggcagc tcgagaattc atcctgaaaa tgttcgtgga cctgaacccc
     4141 gacagtgaca aaatcatcta ctcccacttc acgtgcgcca cagataccga gaacatccgc
     4201 ttcgtctttg cagccgtcaa ggacaccatc ctgcagctga acctgaagga gtacaatctg
     4261 gtctaactcg agccatggaa gcttgatatc taactgactg aaccggtggt accgaggaat
     4321 taattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     4381 gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     4441 cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa tctttttgtt
     4501 gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga caatgacatc
     4561 cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcaggt cgagcatgca
     4621 tctagggcgg ccaattccgc cgatcctgga aaaacatgga gcaatcacaa gtagcaatac
     4681 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     4741 tccagtcaca cctcagacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     4801 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     4861 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     4921 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     4981 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     5041 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     5101 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg
     5161 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     5221 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     5281 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     5341 tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     5401 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5461 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     5521 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     5581 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     5641 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     5701 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     5761 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5821 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5881 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccg
     5941 ctttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6001 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6061 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6121 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6181 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     6241 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6301 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6361 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6421 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6481 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6541 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6601 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6661 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     6721 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     6781 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6841 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6901 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     6961 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     7021 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     7081 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     7141 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     7201 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     7261 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     7321 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     7381 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     7441 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     7501 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     7561 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     7621 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7681 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7741 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7801 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7861 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7921 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7981 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8041 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8101 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8161 ggccaggaac cgtaaaaagg ccgcgttgct ggcttttttc cataggctcc gcccccctga
     8221 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8281 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8341 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8401 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8461 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8521 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8581 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8641 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8701 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8761 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8821 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8881 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8941 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9001 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9061 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9121 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9181 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9241 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9301 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9361 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9421 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9481 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9541 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9601 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9661 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9721 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9781 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9841 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9901 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
