LOCUS       sequence-488704-        9222 bp    DNA     circular SYN 08-OCT-2025
DEFINITION  SRF - Serum response factor gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9222)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE11
FEATURES             Location/Qualifiers
     source          1..9222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1919
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2052..2083
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2110..2119
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2116..3765
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3769..3888
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    3980..4568
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4033..4053)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4451..4462)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4583..4899
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4750..4885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4950..4952,4953..4955,4956..5669)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4995..5016)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5256..5275)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5603..5624
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5741..5974
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6046..6167
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6083..6102)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6137..6156
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6207..6342
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6362..6381)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6363..6381)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6391..6408)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6391..6407)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6400..6422)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6549..7004
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6636..6655)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6846..6867
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7030..7134
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7135..7203,7204..7995)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7353..7372)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8166..8754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8655..8674
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8908..8925
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9042..9063
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9078..9083,9084..9101,9102..9108)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9116..9132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9121..9143
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9140..9156
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9140..9156
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9175..9195
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9177..9195
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
     1921 tcgatcgcac cagcgtgtgg atcggatgtc catattagga catctggatg tccatattag
     1981 gacatctgga tgtccatatt aggacatctg atccgagaac agatctggcc tcggcggcca
     2041 agcttagaca ctagagggta tataatggaa gctcgacttc cagcttggca atccggtact
     2101 gttggtaaag ccaccatgga agatgccaaa aacattaaga agggcccagc gccattctac
     2161 ccactcgaag acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg
     2221 gtgcccggca ccatcgcctt taccgacgca catatcgagg tggacattac ctacgccgag
     2281 tacttcgaga tgagcgttcg gctggcagaa gctatgaagc gctatgggct gaatacaaac
     2341 catcggatcg tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc
     2401 ctgttcatcg gtgtggctgt ggccccagct aacgacatct acaacgagcg cgagctgctg
     2461 aacagcatgg gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag
     2521 atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat ggatagcaag
     2581 accgactacc agggcttcca aagcatgtac accttcgtga cttcccattt gccacccggc
     2641 ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg acaaaaccat cgccctgatc
     2701 atgaacagta gtggcagtac cggattgccc aagggcgtag ccctaccgca ccgcaccgct
     2761 tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca accagatcat ccccgacacc
     2821 gctatcctca gcgtggtgcc atttcaccac ggcttcggca tgttcaccac gctgggctac
     2881 ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg aggaggagct attcttgcgc
     2941 agcttgcaag actataagat tcaatctgcc ctgctggtgc ccacactatt tagcttcttc
     3001 gctaagagca ctctcatcga caagtacgac ctaagcaact tgcacgagat cgccagcggc
     3061 ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca aacgcttcca cctaccaggc
     3121 atccgccagg gctacggcct gacagaaaca accagcgcca ttctgatcac ccccgaaggg
     3181 gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac
     3241 ttggacaccg gtaagacact gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc
     3301 atgatcatga gcggctacgt taacaacccc gaggctacaa acgctctcat cgacaaggac
     3361 ggctggctgc acagcggcga catcgcctac tgggacgagg acgagcactt cttcatcgtg
     3421 gaccggctga agagcctgat caaatacaag ggctaccagg tagccccagc cgaactggag
     3481 agcatcctgc tgcaacaccc caacatcttc gacgccgggg tcgccggcct gcccgacgac
     3541 gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac acggtaaaac catgaccgag
     3601 aaggagatcg tggactatgt ggccagccag gttacaaccg ccaagaagct gcgcggtggt
     3661 gttgtgttcg tggacgaggt gcctaaagga ctgaccggca agttggacgc ccgcaagatc
     3721 cgcgagattc tcattaaggc caagaagggc ggcaagatcg ccgtgaattc tcacggcttc
     3781 cctcccgagg tggaggagca ggccgccggc accctgccca tgagctgcgc ccaggagagc
     3841 ggcatggata gacaccctgc tgcttgcgcc agcgccagga tcaacgtcta aggccgcgac
     3901 tctagaacgc gtcactgcgg ctggagctac gttgcttcag actacagaca tcagcgcgta
     3961 ccggttagta atgatcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4021 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4081 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4141 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4201 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4261 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4321 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     4381 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4441 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4501 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4561 ctccccgcct ggcgatggta ccggtgtgga aagtccccag gctccccagc aggcagaagt
     4621 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     4681 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     4741 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4801 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     4861 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     4921 tccattttcg gatctgatca gcacctagca tggtgagcaa gggcgaggag ctgttcaccg
     4981 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     5041 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     5101 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcacctac ggcgtgcagt
     5161 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     5221 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     5281 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     5341 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaagg
     5401 tctatatcac cgccgacaag cagaagaacg gcatcaaggt gaacttcaag acccgccaca
     5461 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     5521 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     5581 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     5641 ctctcggcat ggacgagctg tacaagtaag gtacctttaa gaccaatgac ttacaaggca
     5701 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     5761 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     5821 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     5881 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     5941 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     6001 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     6061 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6121 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     6181 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6241 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6301 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     6361 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     6421 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6481 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6541 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6601 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6661 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6721 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6781 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6841 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6901 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6961 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     7021 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     7081 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     7141 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     7201 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     7261 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7321 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7381 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7441 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7501 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7561 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7621 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7681 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7741 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7801 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7861 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7921 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7981 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     8041 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     8101 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8161 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8221 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8281 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8341 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8401 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8461 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8521 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8581 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8641 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8701 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8761 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8821 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8881 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8941 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     9001 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     9061 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     9121 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     9181 aaccctcact aaagggaaca aaagctggag ctgcaagctt aa
//
