LOCUS       sequence-488712-        9948 bp    DNA     circular SYN 08-OCT-2025
DEFINITION  Express 3xFlag C-terminal tagged mouse GPR45-Del1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9948)
  AUTHORS   Xun Y, Jiang Y, Xu B, Tang M, Ludwig S, Nakamura K, Mukhopadhyay S, 
            Liu C, Beutler B, Zhang Z
  TITLE     GPR45 modulates Galpha(s) at primary cilia of the paraventricular 
            hypothalamus to control food intake.
  JOURNAL   Science. 2025 Jun 5;388(6751):eadp3989. doi: 
            10.1126/science.adp3989. Epub 2025 Jun 5.
  PUBMED    40472089
REFERENCE   2  (bases 1 to 9948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-06-5"; volume: "388(6751):eadp3989. doi"; pages: " 
            10.1126/science.adp3989. Epub 2025 Jun 5"
COMMENT     Sequence Label: pBOB-Gpr45-Del1&2-3xFlag
FEATURES             Location/Qualifiers
     source          1..9948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3145..3154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4192..4257
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     intron          4349..4578
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4561..4580
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4755..5343
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4808..4828)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5226..5237)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5750..5930
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5956..5973)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5962..6186
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6232..6660
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6319..6338)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6529..6550
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6669..6689)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6674..7003
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6854..6989
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6916..6935
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7051..7098
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7117..7491
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7621..7742
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7658..7677)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7712..7731
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7791..7807)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7791..7807)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7804..7826)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7815..7831
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7839..7845,7846..7863,7864..7869))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7884..7905
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8022..8039)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8193..8781)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8273..8292)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8952..9743,9744..9812))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9575..9594
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9813..9917)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagaattcgg atccgccacc atggcctgta acagcacacc catggggact
     3181 tacgaacatc tgctgctgaa tgtgagcaac actttggacc ctggggacac cccactgtct
     3241 gcaccgctca ggatctcgct ggcaataatg atgctgctga tgatcgtggt aggattcctt
     3301 ggcaacacgg tggtctgcat catcgtgtac cagaggccag ccatgcgttc agctatcaac
     3361 ctgctgctgg ccaccttggc cttctccgac atcatgctgt ctttatgctg catgccattc
     3421 acggccatca ccctcatcac tgttcgctgg catttcgggg accacttttg tcggctttca
     3481 gctactctct attggttttt tgtcctagag ggcgtggcca tcctgctcat cattagcgtg
     3541 gaccggtttc tcatcatcgt gcagcgtcag gacaagctga acccacgcag ggctaagatg
     3601 atcatcgcgg cctcctgggt gctgtctttc tgcatctctg cgccctcctt cactggctgg
     3661 acgttcatgg aggtgcctgc tcgagcccca cagtgcgtgc taggctacac tgagttccca
     3721 gctgaacgcg cctatgtagt gacactggtg gtggcagtgt tctttgctcc cttcggcgtc
     3781 atgttgtgct cctatctgtg catcctcaat acggtgctca gacagctgac cggagctggc
     3841 ctgctgagtt tcaaaaccaa ggccttcacc accatcctca tcctcttcgt gggcttttca
     3901 ctctgctggc tgccacactc agtctacagc ctgctgtctg cgttcagccg gcggttctat
     3961 tacagcgcct ccttctacac caccagcaca tgcgtcctgt ggctcagtta cctcaagtct
     4021 gttttcaacc ccatcgtcta ctgctggagg atcaagaaat tccgcgaggc ctgcatagag
     4081 ttgcttcccc acactttcca aatcctccct aaagtgcctg agcggatcca gaggaaaatc
     4141 cagccaagca ccatctatgt gtgcaacgaa aaccaatccg ctgtcctcga ggactacaaa
     4201 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtag
     4261 tgatcgagcc atggaagctt gatatctaac tgactgaacc ggtggtaccg aggaattaat
     4321 tcgctgtctg cgagggccag ctgttggggt gagtactccc tctcaaaagc gggcatgact
     4381 tctgcgctaa gattgtcagt ttccaaaaac gaggaggatt tgatattcac ctggcccgcg
     4441 gtgatgcctt tgagggtggc cgcgtccatc tggtcagaaa agacaatctt tttgttgtca
     4501 agcttgaggt gtggcaggct tgagatctgg ccatacactt gagtgacaat gacatccact
     4561 ttgcctttct ctccacaggt gtccactccc aggtccaact gcaggtcgag catgcatcta
     4621 gggcggccaa ttccgccgat cctggaaaaa catggagcaa tcacaagtag caatacagca
     4681 gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca
     4741 gtcacacctc agacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4801 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4861 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4921 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4981 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     5041 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     5101 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     5161 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     5221 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     5281 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     5341 cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct
     5401 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     5461 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta
     5521 cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc
     5581 agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc
     5641 tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag
     5701 ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg
     5761 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5821 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5881 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gggccgcttt
     5941 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     6001 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     6061 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     6121 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     6181 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     6241 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     6301 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     6361 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     6421 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     6481 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     6541 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     6601 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     6661 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     6721 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     6781 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     6841 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     6901 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     6961 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     7021 tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc
     7081 ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt
     7141 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     7201 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     7261 ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg
     7321 cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac
     7381 gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg
     7441 cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta
     7501 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     7561 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     7621 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7681 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7741 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7801 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7861 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7921 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7981 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     8041 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     8101 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8161 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8221 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8281 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8341 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8401 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8461 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8521 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8581 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8641 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8701 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8761 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8821 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8881 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8941 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9001 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9061 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9121 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9181 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9241 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9301 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9361 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9421 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9481 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9541 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9601 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9661 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9721 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9781 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9841 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9901 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
