LOCUS       sequence-488717-        9212 bp    DNA     circular SYN 08-OCT-2025
DEFINITION  Circadian - BMAL1/CLOCK gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE30
FEATURES             Location/Qualifiers
     source          1..9212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1802..1919
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2042..2073
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2100..2109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2106..3755
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3759..3878
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    3970..4558
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4023..4043)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4441..4452)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4573..4889
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4740..4875
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4940..4942,4943..4945,4946..5659)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4985..5006)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5246..5265)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5593..5614
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5731..5964
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6036..6157
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6073..6092)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6127..6146
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6197..6332
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6352..6371)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6353..6371)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6381..6398)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6381..6397)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6390..6412)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6539..6994
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6626..6645)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6836..6857
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7020..7124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7125..7193,7194..7985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7343..7362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8156..8744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8645..8664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8898..8915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9032..9053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9068..9073,9074..9091,9092..9098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9106..9122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9111..9133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9130..9146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9130..9146
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9165..9185
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9167..9185
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     1741 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     1801 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     1861 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaatttta
     1921 tcgatcgcac cagcgtgtgg atccggtcac gttttcccgg tcacgttttc ccggtcacgt
     1981 tttcccggtc acgttttccg atccgagaac agatctggcc tcggcggcca agcttagaca
     2041 ctagagggta tataatggaa gctcgacttc cagcttggca atccggtact gttggtaaag
     2101 ccaccatgga agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag
     2161 acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca
     2221 ccatcgcctt taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga
     2281 tgagcgttcg gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg
     2341 tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg
     2401 gtgtggctgt ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg
     2461 gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg
     2521 tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc
     2581 agggcttcca aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt
     2641 acgacttcgt gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta
     2701 gtggcagtac cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat
     2761 tcagtcatgc ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca
     2821 gcgtggtgcc atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg
     2881 gctttcgggt cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag
     2941 actataagat tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca
     3001 ctctcatcga caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc
     3061 tcagcaagga ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg
     3121 gctacggcct gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc
     3181 ctggcgcagt aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg
     3241 gtaagacact gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga
     3301 gcggctacgt taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc
     3361 acagcggcga catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga
     3421 agagcctgat caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc
     3481 tgcaacaccc caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg
     3541 agctgcccgc cgcagtcgtc gtgctggaac acggtaaaac catgaccgag aaggagatcg
     3601 tggactatgt ggccagccag gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg
     3661 tggacgaggt gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc
     3721 tcattaaggc caagaagggc ggcaagatcg ccgtgaattc tcacggcttc cctcccgagg
     3781 tggaggagca ggccgccggc accctgccca tgagctgcgc ccaggagagc ggcatggata
     3841 gacaccctgc tgcttgcgcc agcgccagga tcaacgtcta aggccgcgac tctagaacgc
     3901 gtcactgcgg ctggaggcgg gggctcatcg actacagaca tcagcgcgta ccggttagta
     3961 atgatcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     4021 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     4081 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     4141 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     4201 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     4261 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     4321 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     4381 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4441 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4501 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     4561 ggcgatggta ccggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4621 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4681 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4741 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4801 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     4861 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     4921 gatctgatca gcacctagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4981 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     5041 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     5101 gcccgtgccc tggcccaccc tcgtgaccac cttcacctac ggcgtgcagt gcttcgcccg
     5161 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     5221 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     5281 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     5341 cggcaacatc ctggggcaca agctggagta caactacaac agccacaagg tctatatcac
     5401 cgccgacaag cagaagaacg gcatcaaggt gaacttcaag acccgccaca acatcgagga
     5461 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     5521 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     5581 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     5641 ggacgagctg tacaagtaag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     5701 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     5761 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     5821 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     5881 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     5941 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     6001 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     6061 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     6121 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     6181 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6241 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6301 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     6361 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     6421 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     6481 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     6541 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     6601 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     6661 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     6721 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     6781 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     6841 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     6901 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     6961 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     7021 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7081 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7141 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7201 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7261 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7321 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7381 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7441 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7501 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7561 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7621 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7681 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7741 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7801 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7861 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7921 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7981 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     8041 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8101 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8161 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8221 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8281 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8341 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8401 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8461 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8521 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8581 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8641 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8701 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8761 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8821 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8881 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8941 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     9001 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     9061 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     9121 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     9181 aaagggaaca aaagctggag ctgcaagctt aa
//
