LOCUS       sequence-488720-        9997 bp    DNA     circular SYN 08-OCT-2025
DEFINITION  Express HA N-terminal tagged mouse Galpha15 transiently via 
            transfection or stably via lentivirus-mediated infection in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Xun Y, Jiang Y, Xu B, Tang M, Ludwig S, Nakamura K, Mukhopadhyay S, 
            Liu C, Beutler B, Zhang Z
  TITLE     GPR45 modulates Galpha(s) at primary cilia of the paraventricular 
            hypothalamus to control food intake.
  JOURNAL   Science. 2025 Jun 5;388(6751):eadp3989. doi: 
            10.1126/science.adp3989. Epub 2025 Jun 5.
  PUBMED    40472089
REFERENCE   2  (bases 1 to 9997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2025-06-5"; volume: "388(6751):eadp3989. doi"; pages: " 
            10.1126/science.adp3989. Epub 2025 Jun 5"
COMMENT     Sequence Label: pBOB-HA-Gna15
FEATURES             Location/Qualifiers
     source          1..9997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3142..3168
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          4398..4627
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4610..4629
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4804..5392
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4857..4877)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5275..5286)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5799..5979
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6005..6022)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6011..6235
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6281..6709
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6368..6387)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6578..6599
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6718..6738)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6723..7052
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6903..7038
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6965..6984
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7100..7147
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7166..7540
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7670..7791
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7707..7726)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7761..7780
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7840..7856)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7840..7856)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7853..7875)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7864..7880
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7888..7894,7895..7912,7913..7918))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7933..7954
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8071..8088)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8242..8830)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8322..8341)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9001..9792,9793..9861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9624..9643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9862..9966)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaactcta gagccaccat gtacccatac gatgttccag attacgcttc tagagaattc
     3181 ggatccatgg cccggtccct gacttggggc tgctgtccct ggtgcctgac agaggaggag
     3241 aagactgccg ccagaatcga ccaggagatc aacaggattt tgttggaaca gaaaaaacaa
     3301 gagcgcgagg aattgaaact cctgctgttg gggcctggtg agagcgggaa gagtacgttc
     3361 atcaagcaga tgcgcatcat tcacggtgtg ggctactcgg aggaggaccg cagagccttc
     3421 cggctgctca tctaccagaa catcttcgtc tccatgcagg ccatgataga tgcgatggac
     3481 cggctgcaga tccccttcag caggcctgac agcaagcagc acgccagcct agtgatgacc
     3541 caggacccct ataaagtgag cacattcgag aagccatatg cagtggccat gcagtacctg
     3601 tggcgggacg cgggcatccg tgcatgctac gagcgaaggc gtgaattcca ccttctggac
     3661 tccgcggtgt attacctgtc acacctggag cgcatatcag aggacagcta catccccact
     3721 gcgcaagacg tgctgcgcag tcgcatgccc accacaggca tcaatgagta ctgcttctcc
     3781 gtgaagaaaa ccaaactgcg catcgtggat gttggtggcc agaggtcaga gcgtaggaaa
     3841 tggattcact gtttcgagaa cgtgattgcc ctcatctacc tggcctccct gagcgagtat
     3901 gaccagtgcc tagaggagaa cgatcaggag aaccgcatgg aggagagtct cgctctgttc
     3961 agcacgatcc tagagctgcc ctggttcaag agcacctcgg tcatcctctt cctcaacaag
     4021 acggacatcc tggaagataa gattcacacc tcccacctgg ccacatactt ccccagcttc
     4081 cagggacccc ggcgagacgc agaggccgcc aagagcttca tcttggacat gtatgcgcgc
     4141 gtgtacgcga gctgcgcaga gccccaggac ggtggcagga aaggctcccg cgcgcgccgc
     4201 ttcttcgcac acttcacctg tgccacggac acgcaaagcg tccgcagcgt gttcaaggac
     4261 gtgcgggact cggtgctggc ccggtacctg gacgagatca acctgctgtg actcgagcca
     4321 tggaagcttg atatctaact gactgaaccg gtggtaccga ggaattaatt cgctgtctgc
     4381 gagggccagc tgttggggtg agtactccct ctcaaaagcg ggcatgactt ctgcgctaag
     4441 attgtcagtt tccaaaaacg aggaggattt gatattcacc tggcccgcgg tgatgccttt
     4501 gagggtggcc gcgtccatct ggtcagaaaa gacaatcttt ttgttgtcaa gcttgaggtg
     4561 tggcaggctt gagatctggc catacacttg agtgacaatg acatccactt tgcctttctc
     4621 tccacaggtg tccactccca ggtccaactg caggtcgagc atgcatctag ggcggccaat
     4681 tccgccgatc ctggaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc
     4741 tgattgtgcc tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca
     4801 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4861 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4921 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4981 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5041 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     5101 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     5161 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     5221 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     5281 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     5341 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     5401 cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     5461 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     5521 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     5581 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     5641 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     5701 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     5761 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     5821 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     5881 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     5941 ccctcagacc cttttagtca gtgtggaaaa tctctagcag ggccgcttta aacccgctga
     6001 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     6061 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     6121 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     6181 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     6241 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
     6301 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6361 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6421 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6481 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6541 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6601 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6661 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
     6721 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6781 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     6841 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     6901 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     6961 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     7021 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     7081 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
     7141 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
     7201 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
     7261 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
     7321 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
     7381 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
     7441 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
     7501 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
     7561 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
     7621 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     7681 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7741 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     7801 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     7861 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     7921 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     7981 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     8041 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     8101 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     8161 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     8221 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     8281 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     8341 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     8401 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     8461 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     8521 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     8581 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     8641 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     8701 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     8761 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     8821 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     8881 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     8941 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     9001 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     9061 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     9121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     9181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     9241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     9301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     9361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     9421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     9481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     9541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     9601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     9661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     9721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     9781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     9841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     9901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     9961 tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
