LOCUS       sequence-48875-e         688 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: DIRAS1
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgc cggaacagag taacgattac cgcgtggtgg tgttcggggc gggcggcgtg
      121 ggcaagagct cgctggtgct gcgcttcgtg aagggcacgt tccgcgacac ctacatcccc
      181 accatcgagg acacctaccg gcaggtgatc agctgcgaca agagcgtgtg cacgctgcag
      241 atcacagaca ccaccggcag ccaccagttc ccggccatgc agcgcctgtc catctccaag
      301 ggccacgcct tcatcctggt gttctccgtc accagcaagc agtcgctgga ggagctgggg
      361 cccatctaca agctcatcgt gcagatcaag ggcagcgtgg aggacatccc cgtgatgctc
      421 gtgggcaaca agtgtgatga gacgcagcgg gaggtggaca cgcgcgaggc gcaggcggtg
      481 gcccaggagt ggaagtgcgc tttcatggag acctcggcca agatgaacta caacgtcaag
      541 gagctcttcc aggagctgct gacgctggag acgcgccgga acatgagcct caacatcgac
      601 ggcaagcgct ccgggaagca gaagaggaca gaccgcgtca agggcaaatg caccctcatg
      661 tgacagtaaa ggtggatacg gatccgaa
//
