LOCUS       sequence-488812-        9202 bp    DNA     circular SYN 08-OCT-2025
DEFINITION  vector for encoding a human codon-optimized High-fidelity Cas13X 
            (hfCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X
            driven by hU6, EGFP and mCherry driven by SV40 promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   Tong H, Huang J, Xiao Q, He B, Dong X, Liu Y, Yang X, Han D, Wang Z,
            Wang X, Ying W, Zhang R, Wei Y, Xu C, Zhou Y, Li Y, Cai M, Wang Q, 
            Xue M, Li G, Fang K, Zhang H, Yang H
  TITLE     High-fidelity Cas13 variants for targeted RNA degradation with 
            minimal collateral effects.
  JOURNAL   Nat Biotechnol. 2022 Aug 11. pii: 10.1038/s41587-022-01419-7. doi: 
            10.1038/s41587-022-01419-7.
  PUBMED    35953673
REFERENCE   2  (bases 1 to 9202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Aug 11. pii: 10.1038/s41587-022-01419-7. doi: 
            10.1038/s41587-022-01419-7."
COMMENT     Sequence Label: 
            pCBh_NLS_hfCas13X(Cas13X_M17YY)_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP
            -pA-pSV40-mCherry-pA
FEATURES             Location/Qualifiers
     source          1..9202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(336..1127,1128..1196))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     959..978
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1197..1301)
                     /gene="bla"
                     /label=AmpR promoter
     repeat_region   1332..1366
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1723..1844
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1967..2677)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     2514..2532
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2674..2683
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2724..3081)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2738..2873
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        2890..3081
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     promoter        complement(3083..3154)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    3161..3282
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(3405..4118,4119..4121,4122..4124))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(3408..4118,4119..4121,4122..4124))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3450..3471)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3799..3818
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4058..4079
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4121..4130
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4171..4528)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4185..4320
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4337..4528
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     promoter        complement(4530..4601)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(4718..4966)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(4776..4795)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(4946..4966)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     polyA_signal    4973..5107
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5117..5137)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(7460..7480)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          7501..7728
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     enhancer        8025..8310
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     repeat_region   8619..8681
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      complement(join(8779..9202,1..165))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8859..8878)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
       61 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      121 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      181 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      241 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      301 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      361 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      421 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
      481 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
      541 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
      601 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
      661 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
      721 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
      781 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
      841 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
      901 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
      961 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1021 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1081 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1141 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1201 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1261 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1321 cggatcctta accctagaaa gatagtctgc gtaaaattga cgcatgcatt cttgaaatat
     1381 tgctctctct ttctaaatag cgcgaatccg tcgctgtgca tttaggacat ctcagtcgcc
     1441 gcttggagct cccgtgaggc gtgcttgtca atgcggtaag tgtcactgat tttgaactat
     1501 aacgaccgcg tgagtcaaaa tgacgcatga ttatctttta cgtgactttt aagatttaac
     1561 tcatacgata attatattgt tatttcatgt tctacttacg tgataactta ttatatatat
     1621 attttcttgt tatagatatc gtgactaata tataataaaa gcattacacg tcttgagcga
     1681 ttgtgtaggc tggagctgct tcgtctaggg cgcgccgtcg actaagatac attgatgagt
     1741 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     1801 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     1861 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     1921 tctacaaatg tggtatggct gattatgatc tagagtcgcg gccgctttac ttgtacagct
     1981 cgtccatgcc gccggtggag tggcggccct cggcgcgttc gtactgttcc acgatggtgt
     2041 agtcctcgtt gtgggaggtg atgtccaact tgatgttgac gttgtaggcg ccgggcagct
     2101 gcacgggctt cttggccttg taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct
     2161 tcagcttcag cctctgcttg atctcgccct tcagggcgcc gtcctcgggg tacatccgct
     2221 cggaggaggc ctcccagccc atggttttct tctgcattac ggggccgtcg gaggggaagt
     2281 tggtgccgcg cagcttcacc ttgtagatga actcgccgtc ctgcagggag gagtcctggg
     2341 tcacggtcac cacgccgccg tcatcgaagt tcatcacgcg ctcccacttg aagccctcgg
     2401 ggaaggacag cttcaagtag tcggggatgt cggcggggtg cttcacgtag gccttggagc
     2461 cgtacatgaa ctgaggggac aggatgtccc aggcgaaggg cagggggcca cccttggtca
     2521 ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct
     2581 cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aactccttga
     2641 tgatggccat gttatcctcc tcgcccttgc tcaccatggt ggctctagag cgaaacgatc
     2701 ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     2761 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
     2821 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     2881 gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     2941 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc
     3001 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac
     3061 agctggttct ttccgcctca ggactcttcc tttttcaata ttattgaagc atttatcagg
     3121 gttattgtct catgagcgga tacatatttg aatggtcgac taagatacat tgatgagttt
     3181 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     3241 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     3301 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     3361 tacaaatgtg gtatggctga ttatgatcta gagtcgcggc cgcttcactt gtacagctcg
     3421 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
     3481 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc
     3541 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
     3601 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
     3661 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
     3721 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
     3781 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
     3841 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
     3901 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
     3961 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
     4021 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
     4081 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc tctagagcga
     4141 aacgatcctc atcctgtctc ttgatcgatc tttgcaaaag cctaggcctc caaaaaagcc
     4201 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     4261 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     4321 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     4381 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     4441 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     4501 attccacagc tggttctttc cgcctcagga ctcttccttt ttcaatatta ttgaagcatt
     4561 tatcagggtt attgtctcat gagcggatac atatttgaat gacgcgtaaa aaaagctgta
     4621 atcaccccac aaatcggggg ctgctccagc aggtcttccg acgcttgaat atcgaagacc
     4681 gctgtaatca ccccacaaat cgggggctgc tccagccggt gtttcgtcct ttccacaaga
     4741 tatataaagc caagaaatcg aaatactttc aagttacggt aagcatatga tagtccattt
     4801 taaaacataa ttttaaaact gcaaactacc caagaaatta ttactttcta cgtcacgtat
     4861 tttgtactaa tatctttgtg tttacagtca aattaattcc aattatctct ctaacagcct
     4921 tgtatcgtat atgcaaatat gaaggaatca tgggaaatag gccctcacta gtgatccaga
     4981 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     5041 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     5101 acaagttaag cttctacacc ttccgcttct tcttgggctt gacagggaac ttccattcct
     5161 tctcgatgcc gtacttcttc atcacgtcgc tgaacaggcc gaactcgtca atcacgaact
     5221 tcaggtggtg gtggaagaag gcgcgtctca ccttgttcac tgcggtcttt tcggcctcct
     5281 tgcacatggt ctgggccagg atctcccgga agtcgatgta gtgggcgcct tccttctcgc
     5341 tcatcttctt ggccttcacc accttctctt cgaaagccag cacggcctcc acgcacttct
     5401 tctgcaggtc gttgtatgct ctaaagccct tttcggccac tgtgtgggcc cggatcttgc
     5461 ccttttcgtg gggcatgaag tattcgcaga tccggcccag gaattcggca tcgtccagca
     5521 cgtacagctt gccgtagtca gacacggaga acacgatgct cttctcattt cccacagagc
     5581 cttccacagg cagttcgatg ctgtcgttgc tccacacaat cttgttgccc agttcctttc
     5641 tcacgctgcc caggaagtac tgggccatca tgaggcacag ccgatctctg gccagggtct
     5701 cgtacagggc ggggttggcg ccctcgaatc tgccgatggc gtcgatgtgg tagtacttct
     5761 tgtccaggcc cacgtcgcgc tggccgccgc cggactccag gtgttcctcc accagctttg
     5821 cgaatccctt cttgctatcg taccagaact tctttctcag gaatcctctc cggatgctga
     5881 tgtgctcctt gaaccaggcc accttctgct tgtagtcgat ttcgtccttc ttgcccagtc
     5941 ccacgtaatc gtacagcttc tggtcgccga ttctgcacag ccggttcagg atctggtcgc
     6001 acaccacctg gtgcatctca ttgatggtgc tggtctgggc gaagatggag tacacccggc
     6061 catcgatcct ttcggccagc tgcagccgct tcaggccggc ctgaaagttc tccacgtcct
     6121 tgcccaccag gcacaccagc agtctgttgt attcgccagg gttgaaggac cgggtgcagt
     6181 tttcgttcac gtactgcagg atgtctctgg ccttctcgtg cagggtcatt tcattggtgg
     6241 cggccttctt cttctcccac acgccccgca cgtgcttcac ccgtccatcg atccgctgct
     6301 tgaaggcctt tctgccgatg ccgtgctgct ccagcacgaa gcggggcagg aacacgtggt
     6361 tatccttatc ggtcaccttc accacatcca ggatgttctc cacgtgctgt ctgtatctgt
     6421 acagcttggc gatggcatcg tcgcccttcc cctgcaggct cagcagcacg aggtacttca
     6481 gctcgttcag gcccatccgg tagcttctag ggccggcgtt cttgtcgatc cgcacgatca
     6541 cgttgttctt ggagatatag tagctctggt cctcgtcctt cttgctgaag tccaccacca
     6601 cctttccctt ggtccggtgc ttcttgtgct catcgccggc gccggcctct tcccgcacga
     6661 tgtggcgtct gccgaagcag atctcgctgt gctgggcttc caggtagtgc agtgcaaatt
     6721 cgatgaactt gtccttgtgc cgcttgggat ttgtgccctc attgtcgtta gctctcttct
     6781 tatcaccctg ctcgccgtgg tagtactcgt aggcctcggc ggggattctt cccagctggg
     6841 ccaggatgtc tctgaacagc agcacccgct tatcccaggc cttggtgaat ctgctgtcct
     6901 tcaggcagta catgctcagg gccttccggg tcagcttgta ctgtccctca ttcttcttca
     6961 ggccggacac ggctccatac agccgatcca gcactcttct ttccacgaaa aagctcacga
     7021 aaaacaccac gccggcggtg gtgatccggt cgccctcgaa caggctggga aactcgatga
     7081 tcacctctgt ctctcttctt ctgcactcga agatggcccg ctcataggct ctctccatga
     7141 tggtccgcag ttcgtcctcg gcggtgaagg tcaggcagcc agggctatgc cggtagtgac
     7201 tgaagtagtt ccgcagggct tcagccttgg cgttggcggc ctgggtgctg cacttgatca
     7261 gggcccgggt atcttcgtcg taccagtcgt gcttgctgta cttctcgtgt ctcagcaggc
     7321 tttccacgaa caggccgtcg ttcttgtccc ggttcaccag ggccttgttg aaggcaatgg
     7381 tgaagcacca cttccgggcg ccctggtact cgtcgatgct cagctccctc ttcttggagg
     7441 tctgcttgct cacctgggcc accttccgct tcttcttggg catggtggca ccggtccaac
     7501 ctgaaaaaaa gtgatttcag gcaggtgctc caggtaatta aacattaata ccccaccaac
     7561 caaccatccc ttaaaccctt acctcttgct cagctaatta cagcccggag gagaagggcc
     7621 gtcccgcccg ctcacctgtg ggagtaacgc ggtcagtcag agccggggcg ggcggcgcga
     7681 ggcggcggcg gagcggggca cggggcgaag gcagcgcgca gcgactcccg cccgccgcgc
     7741 gcttcgcttt ttatagggcc gccgccgccg ccgcctcgcc ataaaaggaa actttcggag
     7801 cgcgccgctc tgattggctg ccgccgcacc tctccgcctc gccccgcccc gccccccgcc
     7861 cccccccccc ccgcccggcg cgcgcccccc cccccccccc cccccccccc cccccccgcc
     7921 cccatcgctg cacaaaataa ttaaaaaata aataaataca aaattggggg tggggagggg
     7981 ggggagatgg ggagagtgaa gcagaacgtg gggctcacct cgaccatggt aatagcgatg
     8041 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcacaa
     8101 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     8161 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     8221 aaagtcccta ttggcgttac tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
     8281 ggcgggccat ttaccgtaag ttatgtaacg ggtacctcta gattaattaa taagtataat
     8341 ttgtttctat tatgtataag ttaagctaat tacttatttt ataatacaac atgactgttt
     8401 ttaaagtaca aaataagttt atttttgtaa aagagagaat gtttaaaagt tttgttactt
     8461 tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat aaataaattg
     8521 tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa
     8581 attaataaat aaacctcgat atacagatcg ataaaacaca tgcgtcaatt ttacgcatga
     8641 ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaactcga gagaatcagg
     8701 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8761 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8821 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8881 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8941 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9001 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9061 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9121 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9181 gttcttgaag tggtggccta ac
//
