LOCUS       sequence-488851-       10663 bp    DNA     circular SYN 08-OCT-2025
DEFINITION  To amplify the virus that expresses C-MYC GAC F7 with 33 residues 
            mutated to alanine at the interface for interacting with PDZD8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 10663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pBOBI C-MYC GAC F7
FEATURES             Location/Qualifiers
     source          1..10663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1677..1910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2095..2139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2391..2507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2530..2833
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2834..3037
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3034..3058
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3127..3136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3136..3165
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     intron          5064..5293
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5276..5295
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5470..6058
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5523..5543)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5941..5952)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6465..6645
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6671..6688)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6677..6901
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6947..7375
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7034..7053)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7244..7265
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7384..7404)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7389..7718
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7569..7704
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7631..7650
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7766..7813
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7832..8206
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8336..8457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8373..8392)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8427..8446
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8506..8522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8506..8522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8519..8541)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8530..8546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8554..8560,8561..8578,8579..8584))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8599..8620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8737..8754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8908..9496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8988..9007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9667..10458,10459..10527))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10290..10309
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10528..10632)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
     1561 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1621 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1681 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1741 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1801 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1861 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1921 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1981 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     2041 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     2101 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     2161 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     2221 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     2281 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
     2341 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcaac ttttaaaaga
     2401 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     2461 atacaaacta aagaattaca aaaacaaatt acaaaattca aaattttatc gataagcttg
     2521 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2581 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2641 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2701 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2761 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     2821 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     2881 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2941 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     3001 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     3061 ctggagacgc catccacgct gttttgacct ccatagaaga caccgactga gctccttaag
     3121 gttaacgcca ccatggaaca aaaactcatc tcagaagagg atctgtctag agaattcgga
     3181 tccatgatgc ggctgcgagg ctcggggatg ccgcgggacc tgctcctgcg gtcgcccgca
     3241 ggagtatcgg cgactctgcg gcgggcacag cccttggtca ccctgtgccg gcgtccccga
     3301 ggcgggggac ggccggccgc gggcccggct gccgccgcgc gactccaccc gtggtggggc
     3361 gggggcggct ggccggcgga gcccctcgcg cggggcctgt ccagctctcc ttcggagatc
     3421 ttgcaggagc tgggcaaggg gagcacgcat ccgcagcccg gggtgtcgcc acccgctgcc
     3481 ccggcggcgc ccggccccaa ggacggcccc ggggagacgg acgcgtttgg caacagcgag
     3541 ggcaaagagc tggtggcctc aggtgaaaat aaaataaaac agggtctgtt agctagcgcg
     3601 gcagatgcgc tgttcgctac aattgctgaa gcagcagcga aaatacctgt tcataaattt
     3661 attacagcac tcaaatctac aggattgcga acgtctgatc ccaggttggc agcgtgtatg
     3721 gctatgttaa gattaactct tgcaacaaca tcagatggtg tcatgctaga caaagatctt
     3781 tttaaaaaat gtgttcagag caacattgct ttgttggcag cagcatttgc aagagcgttt
     3841 gtgattcctg cctttatggc ttttacctca gccattgatg agttatatga aagtgctaaa
     3901 aagcagtctg gaggaaaggt tgcagattat attcctcaac tggccaaatt cagtcccgat
     3961 ttgtggggtg tgtctgtttg tacagtagat ggacagaggc attctactgg agataccaaa
     4021 gttcccttct gtcttcagtc ctgtgtaaaa cctttgaaat atgccattgc tgttaatgat
     4081 cttggaactg aatatgtgca tcgatatgtt ggaaaagagc cgagtggact aagattcaac
     4141 aaactatttt tgaatgaaga tgataaacca cataatccta tggtaaatgc tggagcaatt
     4201 gttgtgactt cactaataaa gcaaggagta aataatgctg aaaaatttga ctatgtcatg
     4261 cagtttttga ataagatggc tggtaatgaa tatgttggat tcagtaatgc aacgtttcag
     4321 tctgaaagag aaagtggaga tcgaaatttt gcaataggat attacttaaa agaaaagaag
     4381 tgttttccag aaggcacaga catggttggt atattagact tctacttcca gctgtgctcc
     4441 attgaagtga cttgtgaatc agccagtgtg atggctgcga cactggctaa tggtggtgcc
     4501 tgcccaatta ctgctgcagc agcactgagc gctgaagcag ttcgaaatac attgagtttg
     4561 atgcattcct gtggcatgta tgacttctca gggcagtttg ctttccatgt tggtcttcct
     4621 gcaaaatctg gagttgctgg gggcattctt ttagttgtcc ccaatgttat gggtatgatg
     4681 tgctggtctc ctcctctgga taagatgggc aacagtgtta agggaattca cttttgtcac
     4741 gatcttgttt ctctgtgtaa tttccataac tatgataatt tggcagccgc tgcagcaaaa
     4801 gctgatcctc gagcagcagg tggtgatcaa aggcattcct ttggaccatt ggactatgaa
     4861 agtctccaac aagaacttgc tttaaaagag acagtatgga aaaaagtgtc acctgagtca
     4921 aatgaggaca tctctacaac tgtagtatat agaatggaaa gtctgggaga gaaaagcctc
     4981 gagccatgga agcttgatat ctaactgact gaaccggtgg taccgaggaa ttaattcgct
     5041 gtctgcgagg gccagctgtt ggggtgagta ctccctctca aaagcgggca tgacttctgc
     5101 gctaagattg tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgcggtgat
     5161 gcctttgagg gtggccgcgt ccatctggtc agaaaagaca atctttttgt tgtcaagctt
     5221 gaggtgtggc aggcttgaga tctggccata cacttgagtg acaatgacat ccactttgcc
     5281 tttctctcca caggtgtcca ctcccaggtc caactgcagg tcgagcatgc atctagggcg
     5341 gccaattccg ccgatcctgg aaaaacatgg agcaatcaca agtagcaata cagcagctac
     5401 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     5461 acctcagaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     5521 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     5581 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     5641 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     5701 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     5761 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     5821 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     5881 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     5941 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6001 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     6061 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6121 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata
     6181 tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag aactacacac
     6241 cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     6301 agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga
     6361 gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc
     6421 tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag
     6481 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6541 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6601 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc gctttaaacc
     6661 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     6721 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     6781 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     6841 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     6901 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     6961 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     7021 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     7081 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7141 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7201 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7261 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     7321 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     7381 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     7441 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     7501 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     7561 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     7621 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     7681 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     7741 ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat
     7801 agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt
     7861 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     7921 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     7981 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     8041 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     8101 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     8161 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga
     8221 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     8281 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt
     8341 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     8401 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8461 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     8521 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8581 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8641 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8701 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8761 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8821 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8881 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8941 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     9001 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     9061 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     9121 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     9181 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     9241 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     9301 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     9361 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     9421 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     9481 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9541 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9601 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9661 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9721 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9781 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9841 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9901 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9961 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    10021 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    10081 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    10141 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    10201 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    10261 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    10321 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    10381 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    10441 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    10501 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10561 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10621 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
