LOCUS       sequence-488865-       10442 bp    DNA     circular SYN 08-OCT-2025
DEFINITION  Used for transfection of cells for expression of N-HA ZO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Li M, Wang Y, Wei X, Cai WF, Wu J, Zhu M, Wang Y, Liu YH, Xiong J, 
            Qu Q, Chen Y, Tian X, Yao L, Xie R, Li X, Chen S, Huang X, Zhang C, 
            Xie C, Wu Y, Xu Z, Zhang B, Jiang B, Wang ZC, Li Q, Li G, Lin SY, Yu
            L, Piao HL, Deng X, Han J, Zhang CS, Lin SC
  TITLE     AMPK targets PDZD8 to trigger carbon source shift from glucose to 
            glutamine.
  JOURNAL   Cell Res. 2024 Jun 19. doi: 10.1038/s41422-024-00985-6.
  PUBMED    38898113
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 8, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2024 Jun 19. doi: 10.1038/s41422-024-00985-6."
COMMENT     Sequence Label: pcDNA 3.3 C-HA ZO1
FEATURES             Location/Qualifiers
     source          1..10442
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     678..697
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        678..696
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2451..2459
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6076..6102
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6109..6135
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6139..6165
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(6213..6230)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6219..6443
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6489..6917
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6576..6595)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6786..6807
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6926..6946)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6931..7260
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7111..7246
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7173..7192
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7308..7355
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7374..7772
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7930..8051
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7967..7986)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8021..8040
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8100..8116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8100..8116)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8113..8135)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8124..8140
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8148..8154,8155..8172,8173..8178))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8193..8214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8331..8348)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8502..9090)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8582..8601)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9261..10052,10053..10121))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9884..9903
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10122..10226)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10301..10320)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga acccactgct
      661 tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagttagag
      721 ctccttaagg ttaactctag agaattcgga tccatgtccg ccagagctgc ggccgccaag
      781 agcacagcaa tggaggaaac agctatatgg gaacaacata cagtgacgct tcacagggct
      841 cctggatttg gatttggaat tgcaatatct ggtggacgag ataatcctca ttttcagagt
      901 ggggaaacgt caatagtgat ttcagatgtg ctgaaaggag gaccagctga aggacagcta
      961 caggaaaatg accgagttgc aatggttaac ggagtttcaa tggataatgt tgaacatgct
     1021 tttgctgttc agcaactaag gaaaagtggg aaaaatgcaa aaattacaat tagaaggaag
     1081 aagaaagttc aaataccagt aagtcgtcct gatcctgaac cagtatctga taatgaagaa
     1141 gatagttatg atgaggaaat acatgatcca agaagtggcc ggagtggtgt ggttaacaga
     1201 aggagtgaga agatttggcc gagggataga agtgcaagta gagagaggag cttgtccccg
     1261 cggtcagaca ggcggtcagt ggcttccagc cagcctgcta aacctactaa agtcacactg
     1321 gtgaaatccc ggaaaaatga agaatatggt cttcgattgg caagccatat atttgttaag
     1381 gaaatttcac aagatagttt ggcagcaaga gatggcaata ttcaagaagg tgatgttgta
     1441 ttgaagataa atggtactgt gacagaaaat atgtcattga cagatgcaaa gacattgata
     1501 gaaaggtcta aaggcaaatt aaaaatggta gttcaaagag atgaacgggc tacgctattg
     1561 aatgtccctg atctttctga cagcatccac tctgctaatg cctctgagag agacgacatt
     1621 tcagaaattc agtcactggc atcagatcat tctggtcgat cacacgatag gcctccccgc
     1681 cgcagccggt cacgatctcc tgaccagcgg tcagagcctt ctgatcattc caggcactcg
     1741 ccgcagcagc caagcaatgg cagtctccgg agtagagatg aagagagaat ttctaaacct
     1801 ggggctgtct caactcctgt aaagcatgct gatgatcaca cacctaaaac agtggaagaa
     1861 gttacagttg aaagaaatga gaaacaaaca ccttctcttc cagaaccaaa gcctgtgtat
     1921 gcccaagttg ggcaaccaga tgtggattta cctgtcagtc catctgatgg tgtcctacct
     1981 aattcaactc atgaagatgg gattcttcgg cccagcatga aattggtaaa attcagaaaa
     2041 ggagatagtg tgggtttgcg gctggctggt ggaaatgatg ttggaatatt tgtagctggc
     2101 gttctagaag atagccctgc agccaaggaa ggcttagagg aaggtgatca aattctcagg
     2161 gtaaacaacg tagattttac aaatatcata agagaagaag ccgtcctttt cctgcttgac
     2221 ctccctaaag gagaagaagt gaccatattg gctcagaaga agaaggatgt ttatcgtcgc
     2281 attgtagaat cagatgtagg agattctttc tatattagaa cccattttga atatgaaaag
     2341 gaatctccct atggacttag ttttaacaaa ggagaggtgt tccgtgttgt ggataccttg
     2401 tacaatggaa aactgggctc ttggcttgct attcgaattg gtaaaaatca taaggaggta
     2461 gaacgaggca tcatccctaa taagaacaga gctgagcagc tagccagtgt acagtataca
     2521 cttccaaaaa cagcaggcgg agaccgtgct gacttctgga gattcagagg tcttcgcagc
     2581 tccaagagaa atcttcgaaa aagcagagag gatttgtccg ctcagcctgt tcaaacaaag
     2641 tttccagctt atgaaagagt ggttcttcga gaagctggat ttctgaggcc tgtaaccatt
     2701 tttggaccaa tagctgatgt tgccagagaa aagctggcaa gagaagaacc agatatttat
     2761 caaattgcaa agagtgaacc acgagacgct ggaactgacc aacgtagctc tggcattatt
     2821 cgcctgcata caataaagca aatcatagat caagacaaac atgctttatt agatgtaaca
     2881 ccaaatgcag ttgatcgtct taactatgcc cagtggtatc caattgttgt atttcttaac
     2941 cctgattcta agcaaggagt aaaaacaatg agaatgaggt tatgtccaga atctcggaaa
     3001 agtgccagga agttatacga gcgatctcat aaacttcgta aaaataatca ccatcttttt
     3061 acaactacaa ttaacttaaa ttcaatgaat gatggttggt atggtgcgct gaaagaagca
     3121 attcaacaac agcaaaacca gctggtatgg gtttccgagg gaaaggcgga tggtgctaca
     3181 agtgatgacc ttgatttgca tgatgatcgt ctgtcctacc tgtcagctcc aggtagtgaa
     3241 tactcaatgt atagcacgga cagtagacac acttctgact atgaagacac agacacagaa
     3301 ggcggggcct acactgatca agaactagat gaaactctta atgatgaggt tgggactcca
     3361 ccggagtctg ccattacacg gtcctctgag cctgtaagag aggactcctc tggaatgcat
     3421 catgaaaacc aaacatatcc tccttactca ccacaagcgc agccacaacc aattcataga
     3481 atagactccc ctggatttaa gccagcctct caacagaaag cagaagcttc atctccagtc
     3541 ccttaccttt cgcctgaaac aaacccagca tcatcaacct ctgctgttaa tcataatgta
     3601 aatttaacta atgtcagact ggaggagccc accccagctc cttccacctc ttactcacca
     3661 caagctgatt ctttaagaac accaagtact gaggcagctc acataatgct aagagatcaa
     3721 gaaccatcat tgtcgtcgca tgtagatcca acaaaggtgt atagaaagga tccatatccc
     3781 gaggaaatga tgaggcagaa ccatgttttg aaacagccag ccgttagtca cccagggcac
     3841 aggccagaca aagagcctaa tctgacctat gaaccccaac tcccatacgt agagaaacaa
     3901 gccagcagag acctcgagca gcccacatac agatacgagt cctcaagcta tacggaccag
     3961 ttttctcgaa actatgaaca tcgtctgcga tacgaagatc gcgtccccat gtatgaagaa
     4021 cagtggtcat attatgatga caaacagccc tacccatctc ggccaccttt tgataatcag
     4081 cactctcaag accttgactc cagacagcat cccgaagagt cctcagaacg agggtacttt
     4141 ccacgttttg aagagccagc ccctctgtct tacgacagca gaccacgtta cgaacaggca
     4201 cctagagcat ccgccctgcg gcacgaagag cagccagctc ctgggtatga cacacatggt
     4261 agactcagac cggaagccca gccccaccct tcagcagggc ccaagcctgc agagtccaag
     4321 cagtattttg agcaatattc acgcagttac gagcaagtac caccccaagg atttacctct
     4381 agagcaggtc attttgagcc tctccatggt gctgcagctg tccctccgct gataccttca
     4441 tctcagcata agccagaagc tctgccttca aacaccaaac cactgcctcc acccccaact
     4501 caaaccgaag aagaggaaga tccagcaatg aagccacagt ctgtactcac cagagttaag
     4561 atgtttgaaa acaaaagatc tgcatcctta gagaccaaga aggatgtaaa tgacactggc
     4621 agttttaagc ctccagaagt agcatctaaa ccttcaggtg ctcccatcat tggtcccaaa
     4681 cccacttctc agaatcaatt cagtgaacat gacaaaactc tgtacaggat cccagaacct
     4741 caaaaacctc aactgaagcc acctgaagat attgttcggt ccaatcatta tgaccctgaa
     4801 gaagatgaag aatattatcg aaaacagctg tcatactttg accgaagaag ttttgagaat
     4861 aagcctcctg cacacattgc tgccagccat ctctccgagc ctgcaaagcc agctcattct
     4921 cagaatcaat caaatttttc tagttattct tcaaagggaa agcctcctga agctgatggt
     4981 gtggatagat catttggcga gaaacgctat gaacccatcc aggccactcc ccctcctcct
     5041 ccattgccct cgcagtatgc ccagccatct cagcctgtca ccagcgcgtc tctccacata
     5101 cattctaagg gagcacatgg tgaaggtaat tcagtgtcat tggattttca gaattcctta
     5161 gtgtccaaac cagacccacc tccatctcag aataagccag caactttcag accaccaaac
     5221 cgagaagata ctgctcaggc agctttctat ccccagaaaa gttttccaga taaagcccca
     5281 gttaatggaa ctgaacagac tcagaaaaca gtcactccag catacaatcg attcacacca
     5341 aaaccatata caagttctgc ccgaccattt gaacgcaagt ttgaaagtcc taaattcaat
     5401 cacaatcttc tgccaagtga aactgcacat aaacctgact tgtcttcaaa aactcccact
     5461 tctccaaaaa ctcttgtgaa atcgcacagt ttggcacagc ctcctgagtt tgacagtgga
     5521 gttgaaactt tctctatcca tgcagagaag cctaaatatc aaataaataa tatcagcaca
     5581 gtgcctaaag ctattcctgt gagtccttca gctgtggaag aggatgaaga tgaagatggt
     5641 catactgtgg tggccacagc ccgaggcata tttaacagca atgggggcgt gctgagttcc
     5701 atagaaactg gtgttagtat aattatccct caaggagcca ttcccgaagg agttgagcag
     5761 gaaatctatt tcaaggtctg ccgggacaac agcatccttc cacctttaga taaagagaaa
     5821 ggtgaaacac tgctgagtcc tttggtgatg tgtggtcccc atggcctcaa gttcctgaag
     5881 cctgtggagc tgcgcttacc acactgtgcg tccatgactc ctgacggttg gtcttttgct
     5941 ctaaaatcat ccgactcctc gtcgggtgat cctaaaacct ggcaaaacaa gtgtcttccc
     6001 ggagatccaa attatctcgt tggagcaaac tgtgtttctg tccttattga ccactttctc
     6061 gagccatgga agctttaccc ttatgacgta cctgactatg ctggcgccta cccttatgac
     6121 gtacctgact atgctggata cccttatgac gtacctgact atgctggaga tatctaactg
     6181 actgaaccgg tggtaccaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     6241 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     6301 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     6361 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     6421 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     6481 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6541 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6601 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6661 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6721 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6781 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6841 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6901 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6961 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     7021 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     7081 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     7141 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     7201 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     7261 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat
     7321 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     7381 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca
     7441 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct
     7501 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg
     7561 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga
     7621 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg
     7681 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat
     7741 tgctgccctc tggttatgtg tgggagggct aagcacttcg tggccgagga gcaggactga
     7801 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     7861 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     7921 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7981 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8041 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     8101 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     8161 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     8221 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     8281 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     8341 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8401 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8461 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8521 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8581 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8641 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8701 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8761 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8821 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8881 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8941 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9001 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     9061 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     9121 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     9181 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     9241 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     9301 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     9361 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9421 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9481 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9541 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9601 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9661 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9721 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9781 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9841 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9901 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9961 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10021 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    10081 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    10141 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    10201 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
    10261 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
    10321 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
    10381 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
    10441 gg
//
