LOCUS       sequence-488873-       13633 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Stably expresses a chimeric protein, 
            SUPV3L1(1-199aa)-ALDH18A1(106aa-385aa)-SUPV3L1(451-786aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13633)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 13633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJL191
FEATURES             Location/Qualifiers
     source          1..13633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2610..3198
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2716..2727
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3125..3145
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3223..3825)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3309..3329)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3806..3825
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3837..4337)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3900..3918)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        4424..5602
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4655..5593
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5550..5570
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     LTR             9438..9618
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9644..9661)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9650..9874
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9920..10348
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10007..10026)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10217..10238
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10357..10377)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10362..10691
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10542..10677
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10604..10623
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10739..10786
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10805..11179
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11309..11430
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11346..11365)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11400..11419
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11479..11495)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11479..11495)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11492..11514)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11503..11519
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11527..11533,11534..11551,11552..11557))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11572..11593
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11710..11727)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11881..12469)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11961..11980)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12640..13431,13432..13500))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13263..13282
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13501..13605)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagcgatg cggggaggcg gcccaaaggg agatccgact
     2641 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     2701 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     2761 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     2821 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     2881 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     2941 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     3001 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     3061 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     3121 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     3181 gtaatccaga ggttgattac cgataagctt gatatcgaat tcttaactgg caccgggctt
     3241 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     3301 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     3361 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     3421 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     3481 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     3541 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     3601 gaccgccacg gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     3661 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt
     3721 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac
     3781 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggtgg taccccctgg
     3841 ggagagaggt cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc
     3901 ttgcagaatg cggaacaccg cgcgggcagg aacagggccc acactaccgc cccacacccc
     3961 gcctcccgca ccgccccttc ccggccgctg ctctcggcgc gccctgctga gcagccgcta
     4021 ttggccacag cccatcgcgg tcggcgcgct gccattgctc cctggcgctg tccgtctgcg
     4081 agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag
     4141 gaaccttccc gacttagggg cggagcagga agcgtcgccg gggggcccac aagggtagcg
     4201 gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg
     4261 ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc cctgcgcaaa cccagggctg
     4321 ccttggaaaa ggcgcaaatc gattgcaaag atggataaag ttttaaacag agaggaatct
     4381 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     4441 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4501 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4561 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4621 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     4681 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     4741 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     4801 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     4861 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     4921 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     4981 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     5041 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     5101 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     5161 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     5221 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     5281 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     5341 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     5401 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     5461 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     5521 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     5581 tcttccattt caggtgtcgt gacctgcagg atcgtgcggg acgtggacgg aagaaaaaag
     5641 agagtgagcg agcgccggaa tcagcccggc gtggagtgcg gacccgcggt agtgccaggg
     5701 ggcgaaggcg gcggtggtga ggaagatact ttggttagtg accacatcgc agcatgtcct
     5761 tctcccgtgc cctattgtgg gctcggctcc cggcggggcg ccaggctggc caccgggcag
     5821 ccatctgctc tgcccttcgt ccccactttg ggccctttcc cggggttctg gggcaagttt
     5881 ctgtccttgc caccgcctcc tcctctgcct ccggtggctc caaaatacca aacacgtcct
     5941 tgttcgtgcc cctgactgtg aaacctcagg gccccagcgc cgacggcgac gtcggggccg
     6001 agctaacccg gcctctggac aagaatgaag taaagaaggt cttagacaaa ttttacaaga
     6061 ggaaagaaat tcagaaactg ggtgctgatt atggacttga tgctcgtctc ttccaccaag
     6121 ctttcataag ctttagaaat tatattatgc agtctcattc cctggatgtg gacattcaca
     6181 ttgttttgaa tgatatttgc ttcggtgcag ctcatgcgga tgatttattc ccatttttct
     6241 tgagacatgc caaacaaata tttcctgtgt tggactgtaa ggatgatcta cgtaaaatca
     6301 gtgacttaag aataccacct aactggtacc cagatgctag agccatgcag cagaatcagg
     6361 gcagagagat gatgctggtg accagtggag ccgtagcctt tggcaaacaa cgcttgcgcc
     6421 atgagatcct tctgtctcag agcgtgcggc aggccctcca ctcggggcag aaccagctga
     6481 aagaaatggc aattccagtc ttagaggcac gagcctgtgc agctgccgga cagagtgggc
     6541 tgatggcctt gtatgaggct atgtttaccc agtacagcat ctgtgctgcc cagattttgg
     6601 tgaccaattt ggatttccat gatgagcaga agcgccggaa cctcaatgga acacttcatg
     6661 aactccttag aatgaacatt gtccccattg tcaacacaaa tgatgctgtt gtccccccag
     6721 ctgagcccaa cagtgacctg cagggggtaa atgttattag tgttaaagat aatgatagcc
     6781 tggctgcccg actggctgtg gaaatgaaaa ctgatctctt gattgttctt tcagatgtag
     6841 aaggcctttt tgacagcccc ccaggttcag atgatgcaaa gcttattgat atattttatc
     6901 ccggagatca gcagtctgtg acatttggaa ccaagtctag agtgggaatg ggtggcatgg
     6961 aagccaaggt gaaagcagcc ctctgggctt tgcaaggtgg cacttctgtt gttattgcca
     7021 atggaaccca cccaaaggtg tctgggcacg tcatcacaga cattgtggag gggaagaaag
     7081 ttggtacctt cttttcagaa gtaaagcctg caggccctac tgttgagcag cagggagaaa
     7141 tggcgcgatc tggaggaagg atgttggcca ccttggaacc tgagcagaga aagggagaga
     7201 gagaactaga accaatcaca acctctcaag ccctgcagat tgctggcaga gctggcagat
     7261 tcagctcacg gtttaaagaa ggagaggtta caacaatgaa tcatgaagat ctcagtttat
     7321 taaaggaaat tttgaagagg cctgtggatc ctataagggc agctggtctt catccaactg
     7381 ctgagcagat tgaaatgttt gcctaccatc tccctgatgc aacactgtcc aatctcattg
     7441 atatttttgt agacttttca caagttgatg ggcagtattt tgtctgcaat atggatgatt
     7501 ttaaattttc tgcagagttg atccagcata ttccactaag tctgcgagtg aggtatgttt
     7561 tctgcacagc tcctatcaac aagaagcagc cttttgtgtg ttcttcactg ttacagtttg
     7621 ccaggcagta tagcaggaat gagcccctga cctttgcatg gttacgccga tacatcaaat
     7681 ggcctttact tccacctaag aatattaaag acctcatgga tcttgaagct gtccacgatg
     7741 tcttggatct ttacttgtgg ctaagctacc gatttatgga tatgtttcca gatgccagcc
     7801 ttattcgaga tctccagaaa gaactagatg gtattatcca agatggtgtg cacaatatca
     7861 ctaaattgat taaaatgtct gagacgcata agctgttgaa tttggagggc tttccatcag
     7921 ggagccagtc acgattgtca ggaaccttaa agagccaagc tagaaggaca cgcggcacca
     7981 aagctctagg gagtaaagct actgagccac ccagccccga tgcaggagag ctgtcccttg
     8041 cttccagatt ggtgcagcaa ggactcctca ctccagacat gctgaaacag ctagaaaaag
     8101 agtggatgac acaacaaact gaacacaaca aagaaaaaac agagtctggg actcatccaa
     8161 aagggacgag aagaaagaag aaggaacctg attcggacta gttagtctgc tgaaacaagc
     8221 cgcctgaacg cgttaagtcg acaaagagcc aggaaaaccc gggaattttc caaaaggtct
     8281 tcacgttaaa cttgtcttat ctcaggagag agcccgctct tgtctcccag ttcctggtag
     8341 ggtctgcctg ttggaaagtg tacctggatg cttctgggct ccgtttggca atagcagtct
     8401 tggctgatgt gcacagtctg gctcccagct cacccttttt ttttaaagta agaaaatagt
     8461 tgctaccgat agggactttg ccaagtccaa ttatcttcta ggattgaaag gtgcattttc
     8521 cccataaaaa aggcgaggaa aacccatggc tgctttgtgt cacctcagtg acttacagtc
     8581 ccccttggca tttagttggt actagagcca gtcatcctta acaaatcttt tcacatttta
     8641 tttctttcac atgtagtcat cttcaaaaag gaaagatttg gaattttaga aaaggggcaa
     8701 ctcttctttt tagcattctc atcagaaagt cacaaaaatc gatggaatca tttccactgg
     8761 gaagattgac cttttgtatt tatttgtggg gtaaattaat aagcattcca gatgcttgca
     8821 gcttcctgca tccaggagat gctgtgttcc ccgtgatgca gctggaaccc aagctgcagc
     8881 aggagatgca agtttcagga tgttccccac tgagctggag gaatatctac agcagtgatg
     8941 cttgaaattt ttgtatgaat tattttgtcg tcctaccctt ttcctccaaa acaaaaatta
     9001 gaggattatt ttaatacttt ggattcttcc cccttttttg agattaatta actttaagac
     9061 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     9121 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac
     9181 aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga
     9241 cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg
     9301 aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga
     9361 gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc
     9421 tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     9481 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     9541 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     9601 tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     9661 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     9721 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     9781 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     9841 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     9901 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     9961 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    10021 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    10081 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    10141 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    10201 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    10261 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    10321 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
    10381 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    10441 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    10501 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
    10561 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    10621 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    10681 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt
    10741 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta
    10801 aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc
    10861 ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc
    10921 cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc
    10981 ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc
    11041 ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga
    11101 gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt
    11161 ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga
    11221 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
    11281 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
    11341 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
    11401 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
    11461 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    11521 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    11581 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    11641 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    11701 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    11761 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    11821 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    11881 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    11941 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    12001 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    12061 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    12121 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    12181 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    12241 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    12301 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    12361 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    12421 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    12481 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    12541 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    12601 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    12661 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    12721 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    12781 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    12841 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    12901 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    12961 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    13021 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    13081 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    13141 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    13201 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    13261 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    13321 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    13381 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    13441 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    13501 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    13561 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    13621 agtgccacct gac
//
