LOCUS       sequence-488886-       13111 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Expresses a chimeric form of TBRG4, where the region between residue
            110 and 351 is replaced by a 250-residue XTEN linker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13111)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 13111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJH102
FEATURES             Location/Qualifiers
     source          1..13111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2610..3198
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2716..2727
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3125..3145
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3223..3825)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3309..3329)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3806..3825
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3837..4337)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3900..3918)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        4424..5602
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4655..5593
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5550..5570
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     LTR             8916..9096
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9122..9139)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9128..9352
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9398..9826
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9485..9504)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9695..9716
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9835..9855)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9840..10169
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10020..10155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10082..10101
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10217..10264
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10283..10657
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10787..10908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10824..10843)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10878..10897
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10957..10973)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10957..10973)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10970..10992)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10981..10997
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11005..11011,11012..11029,11030..11035))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11050..11071
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11188..11205)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11359..11947)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11439..11458)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12118..12909,12910..12978))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12741..12760
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12979..13083)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagcgatg cggggaggcg gcccaaaggg agatccgact
     2641 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     2701 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     2761 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     2821 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     2881 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     2941 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     3001 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     3061 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     3121 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     3181 gtaatccaga ggttgattac cgataagctt gatatcgaat tcttaactgg caccgggctt
     3241 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     3301 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     3361 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     3421 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     3481 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     3541 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     3601 gaccgccacg gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     3661 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt
     3721 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac
     3781 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggtgg taccccctgg
     3841 ggagagaggt cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc
     3901 ttgcagaatg cggaacaccg cgcgggcagg aacagggccc acactaccgc cccacacccc
     3961 gcctcccgca ccgccccttc ccggccgctg ctctcggcgc gccctgctga gcagccgcta
     4021 ttggccacag cccatcgcgg tcggcgcgct gccattgctc cctggcgctg tccgtctgcg
     4081 agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag
     4141 gaaccttccc gacttagggg cggagcagga agcgtcgccg gggggcccac aagggtagcg
     4201 gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg
     4261 ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc cctgcgcaaa cccagggctg
     4321 ccttggaaaa ggcgcaaatc gattgcaaag atggataaag ttttaaacag agaggaatct
     4381 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     4441 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4501 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4561 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4621 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     4681 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     4741 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     4801 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     4861 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     4921 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     4981 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     5041 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     5101 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     5161 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     5221 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     5281 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     5341 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     5401 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     5461 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     5521 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     5581 tcttccattt caggtgtcgt gacctgcagg atcgtgcggg acgtggacgg aagaaaaaag
     5641 agagtgagcg agcgccggaa tcagcccggc gtggagtgcg gacccgcggt agtgccaggg
     5701 ggcgaaggcg gcggtggtga ggaagatact ttggttagtg accacatcgc agcatggcag
     5761 ctcacctggt aaagcgatgc acgtgcctcc tgagagaagc tgctcgtcag gcccctgcca
     5821 tggctccagt tggccgactg agacttgcct gggtagccca taagactctg acttcctcag
     5881 ccacctcacc catttcccac ctcccaggtt ccttgatgga gccggtggag aaggaacgag
     5941 catctactcc ctacatagag aagcaggtgg accacctcat caagaaggcc acaaggccag
     6001 aggagctcct ggagctactt ggtggcagtc acgacttgga cagcaatcaa gcagcaatgg
     6061 tacttatccg gctctctcac ttggggtcag aacctgcaac atcaggatcc gagaccccgg
     6121 ggacgtcaga gtcagcaacc cccgaatctg gaccaggcac gagcactgag ccgtcagaag
     6181 ggtcagcacc tggtagtcca gccggaagtc caacgagcac agaagagggc acaagtgagt
     6241 ccgcaacccc cgagagcggc ccaggaagcg agcctgcgac atccggcagc gagaccccgg
     6301 gcacctcaga atcagcaact ccggagagtg gaccgggtag cccagccgga tctcccacta
     6361 gtacggaaga ggggtccccc gcaggctcac ccacgtccac cgaggagggt acttctacgg
     6421 aaccttctga ggggtccgca ccaggtacct ctgaatctgc aacgcccgag tccggtccag
     6481 gaacgtcaga gagtgcaact ccggaatctg gcccgggcac cagtgaatct gccactcctg
     6541 aatccggtcc gggctctgag ccagccacat cagggtctga aacaccgggg agcgaacccg
     6601 cgactagtgg aagtgaaaca cctgggtccc cagccgggtc tccgaccagt actgaggagg
     6661 gaacgtcaac agaaccatcc gagggtagcg cccctgggac ttcaacggag cctagtgaag
     6721 gcagcgcgcc ggggtctgaa cccgcgacca gtggtagcga gacgccgggc acatctgagt
     6781 ccgccacacc agagtctggg cccggtacga gtacagaacc atctgagggc agtgaggcct
     6841 ttgcccagca cgtcctgaac agagcgcagg acatcaccct gccccacctg tgcagcgtac
     6901 ttctggcttt tgcgcgtctg aacttccatc cagaccaaga ggatcagttc ttcagcctgg
     6961 tacatgagaa gctggggtca gagctgccag gcctggagcc agccctgcag gtggacctgg
     7021 tgtgggccct gtgtgtgctg cagcaggcac gggaagcaga gctgcaagcc gtcctccacc
     7081 ctgaatttca catccaattt ctagggggca agtctcagaa ggatcagaac accttccaga
     7141 agctgctcca catcaacgcc actgccctgc tggagtaccc cgagtactcg ggtccccttc
     7201 tgcctgcctc ggctgtggcc cctgggccct cagcccttga caggaaggtg acccccctgc
     7261 aaaaggagct gcaggagacg ctgaaggggc tgctggggag cgccgacaag ggcagcctcg
     7321 aggtggccac gcagtatggc tgggtgctgg atgctgaggt gctgctggac agtgacggcg
     7381 agtttctgcc cgtaagggac tttgtggcac ctcaccttgc ccagccaact gggagccagt
     7441 caccacctcc agggtctaag aggctagcgt tcttgcggtg ggagttcccc aacttcaaca
     7501 gccgaagcaa ggacttgctg ggtcgctttg ttctggcccg gcgacacata gtggctgcag
     7561 gcttcctgat agtggacgtc ccattctatg agtggctgga actcaagtct gaatggcaga
     7621 aaggcgccta cctcaaggac aagatgcgca aagcggtggc tgaggagctg gccaagtgat
     7681 tagtctgctg aaacaagccg cctgaacgcg ttaagtcgac aaagagccag gaaaacccgg
     7741 gaattttcca aaaggtcttc acgttaaact tgtcttatct caggagagag cccgctcttg
     7801 tctcccagtt cctggtaggg tctgcctgtt ggaaagtgta cctggatgct tctgggctcc
     7861 gtttggcaat agcagtcttg gctgatgtgc acagtctggc tcccagctca cccttttttt
     7921 ttaaagtaag aaaatagttg ctaccgatag ggactttgcc aagtccaatt atcttctagg
     7981 attgaaaggt gcattttccc cataaaaaag gcgaggaaaa cccatggctg ctttgtgtca
     8041 cctcagtgac ttacagtccc ccttggcatt tagttggtac tagagccagt catccttaac
     8101 aaatcttttc acattttatt tctttcacat gtagtcatct tcaaaaagga aagatttgga
     8161 attttagaaa aggggcaact cttcttttta gcattctcat cagaaagtca caaaaatcga
     8221 tggaatcatt tccactggga agattgacct tttgtattta tttgtggggt aaattaataa
     8281 gcattccaga tgcttgcagc ttcctgcatc caggagatgc tgtgttcccc gtgatgcagc
     8341 tggaacccaa gctgcagcag gagatgcaag tttcaggatg ttccccactg agctggagga
     8401 atatctacag cagtgatgct tgaaattttt gtatgaatta ttttgtcgtc ctaccctttt
     8461 cctccaaaac aaaaattaga ggattatttt aatactttgg attcttcccc cttttttgag
     8521 attaattaac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8581 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     8641 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     8701 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     8761 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     8821 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     8881 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     8941 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9001 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9061 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     9121 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     9181 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     9241 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     9301 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     9361 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     9421 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     9481 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9541 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     9601 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9661 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9721 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     9781 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     9841 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9901 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9961 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
    10021 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
    10081 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
    10141 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
    10201 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
    10261 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
    10321 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
    10381 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
    10441 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
    10501 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
    10561 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
    10621 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
    10681 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
    10741 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
    10801 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10861 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
    10921 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    10981 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    11041 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    11101 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    11161 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    11221 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    11281 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    11341 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    11401 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    11461 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    11521 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    11581 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    11641 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    11701 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    11761 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    11821 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    11881 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    11941 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    12001 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    12061 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    12121 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    12181 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    12241 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    12301 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    12361 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    12421 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    12481 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    12541 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    12601 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    12661 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    12721 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    12781 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    12841 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    12901 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    12961 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    13021 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    13081 gcgcacattt ccccgaaaag tgccacctga c
//
