LOCUS       sequence-488889-       13546 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Stably expresses SUPV3L1 CDS, flanked with endogenous 5' and 3'UTRs 
            of ALDH18A1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13546)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 13546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJL179
FEATURES             Location/Qualifiers
     source          1..13546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2610..3198
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2716..2727
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3125..3145
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3223..3825)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3309..3329)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3806..3825
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3837..4337)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3900..3918)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        4424..5602
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4655..5593
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5550..5570
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     LTR             9351..9531
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9557..9574)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9563..9787
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9833..10261
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9920..9939)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10130..10151
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10270..10290)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10275..10604
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10455..10590
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10517..10536
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10652..10699
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10718..11092
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11222..11343
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11259..11278)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11313..11332
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11392..11408)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11392..11408)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11405..11427)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11416..11432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11440..11446,11447..11464,11465..11470))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11485..11506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11623..11640)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11794..12382)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11874..11893)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12553..13344,13345..13413))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13176..13195
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13414..13518)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagcgatg cggggaggcg gcccaaaggg agatccgact
     2641 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     2701 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     2761 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     2821 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     2881 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     2941 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     3001 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     3061 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     3121 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     3181 gtaatccaga ggttgattac cgataagctt gatatcgaat tcttaactgg caccgggctt
     3241 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     3301 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     3361 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     3421 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     3481 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     3541 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     3601 gaccgccacg gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     3661 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt
     3721 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac
     3781 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggtgg taccccctgg
     3841 ggagagaggt cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc
     3901 ttgcagaatg cggaacaccg cgcgggcagg aacagggccc acactaccgc cccacacccc
     3961 gcctcccgca ccgccccttc ccggccgctg ctctcggcgc gccctgctga gcagccgcta
     4021 ttggccacag cccatcgcgg tcggcgcgct gccattgctc cctggcgctg tccgtctgcg
     4081 agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag
     4141 gaaccttccc gacttagggg cggagcagga agcgtcgccg gggggcccac aagggtagcg
     4201 gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg
     4261 ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc cctgcgcaaa cccagggctg
     4321 ccttggaaaa ggcgcaaatc gattgcaaag atggataaag ttttaaacag agaggaatct
     4381 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     4441 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4501 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4561 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4621 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     4681 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     4741 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     4801 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     4861 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     4921 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     4981 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     5041 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     5101 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     5161 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     5221 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     5281 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     5341 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     5401 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     5461 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     5521 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     5581 tcttccattt caggtgtcgt gacctgcagg atcgtgcggg acgtggacgg aagaaaaaag
     5641 agagtgagcg agcgccggaa tcagcccggc gtggagtgcg gacccgcggt agtgccaggg
     5701 ggcgaaggcg gcggtggtga ggaagatact ttggttagtg accacatcgc agcatgtcct
     5761 tctcccgtgc cctattgtgg gctcggctcc cggcggggcg ccaggctggc caccgggcag
     5821 ccatctgctc tgcccttcgt ccccactttg ggccctttcc cggggttctg gggcaagttt
     5881 ctgtccttgc caccgcctcc tcctctgcct ccggtggctc caaaatacca aacacgtcct
     5941 tgttcgtgcc cctgactgtg aaacctcagg gccccagcgc cgacggcgac gtcggggccg
     6001 agctaacccg gcctctggac aagaatgaag taaagaaggt cttagacaaa ttttacaaga
     6061 ggaaagaaat tcagaaactg ggtgctgatt atggacttga tgctcgtctc ttccaccaag
     6121 ctttcataag ctttagaaat tatattatgc agtctcattc cctggatgtg gacattcaca
     6181 ttgttttgaa tgatatttgc ttcggtgcag ctcatgcgga tgatttattc ccatttttct
     6241 tgagacatgc caaacaaata tttcctgtgt tggactgtaa ggatgatcta cgtaaaatca
     6301 gtgacttaag aataccacct aactggtacc cagatgctag agccatgcag cggaagataa
     6361 tatttcattc aggccccaca aacagtggaa agacttatca cgcaatccag aaatacttct
     6421 cagcaaagtc tggagtgtat tgtggccctc taaaattact ggcacatgag atcttcgaaa
     6481 agagtaatgc tgctggtgtg ccatgtgact tggtgacagg tgaagagcgt gtgacagttc
     6541 agccaaatgg gaaacaggct tcacatgttt cttgtacagt tgagatgtgc agtgttacaa
     6601 ctccttatga agtggctgta attgatgaaa ttcaaatgat tagagatcca gccagaggat
     6661 gggcctggac cagagcactt ctaggactgt gtgctgaaga ggttcatttg tgtggagaac
     6721 ctgctgctat tgacctggtg atggagctta tgtacacaac gggggaggaa gtggaggttc
     6781 gagactataa gaggcttacc cccatttctg tgctggacca tgcactagaa tctttagata
     6841 accttcggcc tggggactgc attgtctgtt ttagcaagaa tgatatttat tctgtgagtc
     6901 ggcagattga aattcgggga ttagaatcag ctgttatata tggcagtctc ccacctggga
     6961 ccaaacttgc tcaagcaaaa aagtttaatg atcccaatga cccatgcaaa atcttggttg
     7021 ctacagatgc aattggcatg ggacttaatt tgagcataag gagaattatt ttttactccc
     7081 ttataaagcc cagtatcaat gaaaagggag agagagaact agaaccaatc acaacctctc
     7141 aagccctgca gattgctggc agagctggca gattcagctc acggtttaaa gaaggagagg
     7201 ttacaacaat gaatcatgaa gatctcagtt tattaaagga aattttgaag aggcctgtgg
     7261 atcctataag ggcagctggt cttcatccaa ctgctgagca gattgaaatg tttgcctacc
     7321 atctccctga tgcaacactg tccaatctca ttgatatttt tgtagacttt tcacaagttg
     7381 atgggcagta ttttgtctgc aatatggatg attttaaatt ttctgcagag ttgatccagc
     7441 atattccact aagtctgcga gtgaggtatg ttttctgcac agctcctatc aacaagaagc
     7501 agccttttgt gtgttcttca ctgttacagt ttgccaggca gtatagcagg aatgagcccc
     7561 tgacctttgc atggttacgc cgatacatca aatggccttt acttccacct aagaatatta
     7621 aagacctcat ggatcttgaa gctgtccacg atgtcttgga tctttacttg tggctaagct
     7681 accgatttat ggatatgttt ccagatgcca gccttattcg agatctccag aaagaactag
     7741 atggtattat ccaagatggt gtgcacaata tcactaaatt gattaaaatg tctgagacgc
     7801 ataagctgtt gaatttggag ggctttccat cagggagcca gtcacgattg tcaggaacct
     7861 taaagagcca agctagaagg acacgcggca ccaaagctct agggagtaaa gctactgagc
     7921 cacccagccc cgatgcagga gagctgtccc ttgcttccag attggtgcag caaggactcc
     7981 tcactccaga catgctgaaa cagctagaaa aagagtggat gacacaacaa actgaacaca
     8041 acaaagaaaa aacagagtct gggactcatc caaaagggac gagaagaaag aagaaggaac
     8101 ctgattcgga ctagttagtc tgctgaaaca agccgcctga acgcgttaag tcgacaaaga
     8161 gccaggaaaa cccgggaatt ttccaaaagg tcttcacgtt aaacttgtct tatctcagga
     8221 gagagcccgc tcttgtctcc cagttcctgg tagggtctgc ctgttggaaa gtgtacctgg
     8281 atgcttctgg gctccgtttg gcaatagcag tcttggctga tgtgcacagt ctggctccca
     8341 gctcaccctt tttttttaaa gtaagaaaat agttgctacc gatagggact ttgccaagtc
     8401 caattatctt ctaggattga aaggtgcatt ttccccataa aaaaggcgag gaaaacccat
     8461 ggctgctttg tgtcacctca gtgacttaca gtcccccttg gcatttagtt ggtactagag
     8521 ccagtcatcc ttaacaaatc ttttcacatt ttatttcttt cacatgtagt catcttcaaa
     8581 aaggaaagat ttggaatttt agaaaagggg caactcttct ttttagcatt ctcatcagaa
     8641 agtcacaaaa atcgatggaa tcatttccac tgggaagatt gaccttttgt atttatttgt
     8701 ggggtaaatt aataagcatt ccagatgctt gcagcttcct gcatccagga gatgctgtgt
     8761 tccccgtgat gcagctggaa cccaagctgc agcaggagat gcaagtttca ggatgttccc
     8821 cactgagctg gaggaatatc tacagcagtg atgcttgaaa tttttgtatg aattattttg
     8881 tcgtcctacc cttttcctcc aaaacaaaaa ttagaggatt attttaatac tttggattct
     8941 tccccctttt ttgagattaa ttaactttaa gaccaatgac ttacaaggca gctgtagatc
     9001 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     9061 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     9121 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     9181 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     9241 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     9301 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     9361 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9421 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9481 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt
     9541 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     9601 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     9661 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     9721 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     9781 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     9841 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     9901 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     9961 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
    10021 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
    10081 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
    10141 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
    10201 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
    10261 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
    10321 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    10381 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    10441 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    10501 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
    10561 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
    10621 gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg catagtatat
    10681 cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt
    10741 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
    10801 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
    10861 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
    10921 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
    10981 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
    11041 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gacacgtgct
    11101 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
    11161 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
    11221 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
    11281 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
    11341 ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct
    11401 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
    11461 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
    11521 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
    11581 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
    11641 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
    11701 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
    11761 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
    11821 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
    11881 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
    11941 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
    12001 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
    12061 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
    12121 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
    12181 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
    12241 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
    12301 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
    12361 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
    12421 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
    12481 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
    12541 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
    12601 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
    12661 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
    12721 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
    12781 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
    12841 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
    12901 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
    12961 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
    13021 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
    13081 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
    13141 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    13201 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    13261 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    13321 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    13381 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    13441 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    13501 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgac
//
