LOCUS       sequence-488911-       11610 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Firefly luciferase driven by an AHR responsive minimal promoter and 
            a renilla luciferase and GFP driven by a constitutive UBC promoter 
            flipped with respect to the lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Verma M, Desai K, Ding Y, Wang X, Bae M, Rakoff-Nahoum S, Balskus 
            EP, Fischbach MA, Jost M
  TITLE     Systematic identification and characterization of regulators of aryl
            hydrocarbon receptor signaling
  JOURNAL   bioRxiv 2025.09.15.676391
REFERENCE   2  (bases 1 to 11610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.15.676391"
COMMENT     Sequence Label: pHL047 [15ARE23bp -p(TATA)-fLuc-UBC-rLuc-P2A-GFP]
FEATURES             Location/Qualifiers
     source          1..11610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(96..115)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      909..1497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1398..1417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1651..1668
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1908..1929
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1944..1949,1950..1967,1968..1974)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1982..1998
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1987..2009
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2006..2022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2006..2022
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2072..2091
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2283..2916
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2963..3088
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3585..3818
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4003..4047
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4340..4457
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    4529..4577
                     /note="synthetic polyadenylation signal"
     CDS             complement(4623..4649)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(join(4653..5363,5364..5366,5367..5369))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4695..4716)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5044..5063
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5303..5324
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5366..5375
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5376..5432)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(5433..6365)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     regulatory      6362..6371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6406..7617)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     complement(6550..6569)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     polyA_signal    7689..7913
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(7955..9607)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      9604..9613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9636..9667)
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     misc_feature    10282..10870
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10335..10355)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10753..10764)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10945..11178
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11297..11315)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11383..11487
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11488..11556,11557..11610,1..738)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
       61 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      121 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      181 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      241 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
      301 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
      361 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
      421 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
      481 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
      541 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
      601 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
      661 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
      721 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
      781 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      841 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
      901 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      961 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1021 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1081 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1141 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1201 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1261 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1321 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1381 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1441 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1501 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1561 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     1621 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     1681 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc
     1741 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     1801 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg
     1861 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     1921 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     1981 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca
     2041 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     2101 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     2161 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
     2221 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
     2281 gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca
     2341 cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc
     2401 actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc
     2461 caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     2521 ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg
     2581 agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc
     2641 cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag
     2701 atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     2761 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     2821 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     2881 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     2941 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     3001 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     3061 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     3121 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     3181 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     3241 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     3301 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     3361 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     3421 gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
     3481 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
     3541 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
     3601 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
     3661 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
     3721 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
     3781 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
     3841 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
     3901 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
     3961 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
     4021 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
     4081 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
     4141 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
     4201 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
     4261 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgccg aattcacaaa
     4321 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     4381 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     4441 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggactagga
     4501 tccggtaccg agctcttacg cgtcgattca cacaaaaaac caacacacag atgtaatgaa
     4561 aataaagata ttttattgcg gccatcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     4621 ttggcataat cgggcacgtc atagggatag gccttgtaca gctcgtccat gccgagagtg
     4681 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
     4741 ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
     4801 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg
     4861 tggcggatct tgaagttcgc cttgatgccg ttcttctgct tgtcggccat gatatagacg
     4921 ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag
     4981 tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg
     5041 cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg
     5101 ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac
     5161 tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg
     5221 gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccagac
     5281 acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg
     5341 gtgaacagct cctcgccctt gctcaccatg gtggcaggtc cagggttctc ctccacgtct
     5401 ccagcctgct tcagcaggct gaagttagta gcctgctcgt tcttcagcac gcgctccacg
     5461 aagctcttga tgtacttacc catttcatct ggagcgtcct cctggctgaa gtggaggccc
     5521 ttcaccttca cgaactcggt gttagggaac ttcttagctc cctcgacaat agcgttggaa
     5581 aagaacccag ggtcggactc gatgaacatc ttaggcagat cgtcgctggc ccgaaggtag
     5641 gcgttgtagt tgcggacaat ctggacgacg tcgggcttgc ctcccttaac gagagggatc
     5701 tcgcgaggcc aggagagggt aggccgtcta acctcgccct tctccttgaa tggctccagg
     5761 taggcagcga actcctcagg ctccagtttc cgcatgatct tgcttgggag catggtctcg
     5821 acgaagaagt tattctcaag caccattttc tcgccctctt cgctcttgat cagggcgata
     5881 tcctcctcga tgtcaggcca ctcgtcccag gactcgatca cgtccacgac actctcagca
     5941 tggacgatgg ccttgatctt gtcttggtgc tcgtaggagt agtgaaaggc cagacaagcc
     6001 ccccagtcgt ggcccacaaa gatgattttc tttggaaggt tcagcagctc gaaccaagcg
     6061 gtgaggtact tgtagtgatc caggaggcga tatgagccat tcccgctctt gccggactta
     6121 cccattccga tcagatcagg gatgatgcat ctagccacgg gctcgatgtg aggcacgacg
     6181 tgcctccaca ggtagctgga ggcagcgtta ccatgcagaa aaatcacggc gttctcggcg
     6241 tgcttctcgg aatcatagta gttgatgaag gagtccagca cgttcatttg cttgcagcga
     6301 gcccaccact gaggcccagt gatcatgcgt ttgcgttgct cggggtcgta caccttggaa
     6361 gccatggtgg cacggggtgg agatccgagc tcggtaccaa gcttcgtcta acaaaaaagc
     6421 caaaaacggc cagaatttag cggacaattt actagtctaa cactgaaaat tacatattga
     6481 cccaaatgat tacatttcaa aaggtgccta aaaaacttca caaaacacac tcgccaaccc
     6541 cgagcgcata gttcaaaacc ggagcttcag ctacttaaga agataggtac ataaaaccga
     6601 ccaaagaaac tgacgcctca cttatccctc ccctcaccag aggtccggcg cctgtcgatt
     6661 caggagagcc taccctaggc ccgaaccctg cgtcctgcga cggagaaaag cctaccgcac
     6721 acctaccggc aggtggcccc accctgcatt ataagccaac agaacgggtg acgtcacgac
     6781 acgacgaggg cgcgcgctcc caaaggtacg ggtgcactgc ccaacggcac cgccataact
     6841 gccgcccccg caacagacga caaaccgagt tctccagtca gtgacaaact tcacgtcagg
     6901 gtccccagat ggtgccccag cccatctcac ccgaataaga gctttcccgc attagcgaag
     6961 gcctcaagac cttgggttct tgccgcccac catgcccccc accttgtttc aacgacctca
     7021 cagcccgcct cacaagcgtc ttccattcaa gactcgggaa cagccgccat tttgctgcgc
     7081 tccccccaac ccccagttca gggcaacctt gctcgcggac ccagactaca gcccttggcg
     7141 gtctctccac acgcttccgt cccaccgagc ggcccggcgg ccacgaaagc cccggccagc
     7201 ccagcagccc gctactcacc aagtgacgat cacagcgatc cacaaacaag aaccgcgacc
     7261 caaatcccgg ctgcgacgga actagctgtg ccacacccgg cgcgtcctta tataatcatc
     7321 ggcgttcacc gccccacgga gatccctccg cagaatcgcc gagaagggac tacttttcct
     7381 cgcctgttcc gctctctgga aagaaaacca gtgccctaga gtcacccaag tcccgtccta
     7441 aaatgtcctt ctgctgatac tggggttcta aggccgagtc ttatgagcag cgggccgctg
     7501 tcctgagcgt ccgggcggaa ggatcaggac gctcgctgcg cccttcgtct gacgtggcag
     7561 cgctcgccgt gaggaggggg gcgcccgcgg gaggcgccaa aacccggcgc ggaggccaga
     7621 tcttgggtgg gttactccag actgccttgg gaaaagcgcc tcccctaccc ggtagaattt
     7681 ctagtttacc atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc
     7741 ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg
     7801 cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa
     7861 ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgggct
     7921 ctttggttga ggggaagggt ttaaacaact agaattacac ggcgatcttg ccgcccttct
     7981 tggccttaat gagaatctcg cggatcttgc gggcgtccaa cttgccggtc agtcctttag
     8041 gcacctcgtc cacgaacaca acaccaccgc gcagcttctt ggcggttgta acctggctgg
     8101 ccacatagtc cacgatctcc ttctcggtca tggttttacc gtgttccagc acgacgactg
     8161 cggcgggcag ctcgccggca tcgtcgtcgg gcaggccggc gaccccggcg tcgaagatgt
     8221 tggggtgttg cagcaggatg ctctccagtt cggctggggc tacctggtag cccttgtatt
     8281 tgatcaggct cttcagccgg tccacgatga agaagtgctc gtcctcgtcc cagtaggcga
     8341 tgtcgccgct gtgcagccag ccgtccttgt cgatgagagc gtttgtagcc tcggggttgt
     8401 taacgtagcc gctcatgatc atggggccac ggacgcacag ctcgccgcgc tggttcacac
     8461 ccagtgtctt accggtgtcc aagtccacca ccttagcctc gaagaagggc accaccttgc
     8521 ctactgcgcc aggcttgtcg tccccttcgg gggtgatcag aatggcgctg gttgtttctg
     8581 tcaggccgta gccctggcgg atgcctggta ggtggaagcg tttggccacg gcctcaccta
     8641 cctccttgct gagcggcgcc ccgccgctgg cgatctcgtg caagttgctt aggtcgtact
     8701 tgtcgatgag agtgctctta gcgaagaagc taaatagtgt gggcaccagc agggcagatt
     8761 gaatcttata gtcttgcaag ctgcgcaaga atagctcctc ctcgaagcgg tacatgagca
     8821 cgacccgaaa gccgcagatc aagtagccca gcgtggtgaa catgccgaag ccgtggtgaa
     8881 atggcaccac gctgaggata gcggtgtcgg ggatgatctg gttgccgaag atggggtcgc
     8941 gggcatgact gaatcggaca caagcggtgc ggtgcggtag ggctacgccc ttgggcaatc
     9001 cggtactgcc actactgttc atgatcaggg cgatggtttt gtcccggtcg aagctctcgg
     9061 gcacgaagtc gtactcgttg aagccgggtg gcaaatggga agtcacgaag gtgtacatgc
     9121 tttggaagcc ctggtagtcg gtcttgctat ccatgatgat gatcttttgt atgatcggta
     9181 gcttcttttg cacgttgagg atcttttgca gccctttctt gctcacgaat acgacggtgg
     9241 gctggctgat gcccatgctg ttcagcagct cgcgctcgtt gtagatgtcg ttagctgggg
     9301 ccacagccac accgatgaac agggcaccca acacgggcat gaagaactgc aagctattct
     9361 cgctgcacac cacgatccga tggtttgtat tcagcccata gcgcttcata gcttctgcca
     9421 gccgaacgct catctcgaag tactcggcgt aggtaatgtc cacctcgata tgtgcgtcgg
     9481 taaaggcgat ggtgccgggc accagggcgt agcgcttcat ggctttgtgc agctgctcgc
     9541 cggcggtccc gtcttcgagt gggtagaatg gcgctgggcc cttcttaatg tttttggcat
     9601 cttccatggt ggcgcggtac cgcaagcttg atatcctgga agtcgagctt ccattatata
     9661 ccctctacag atctcgagga atcgcacgcc agttaccgaa ctgaacacca cctacacgcg
     9721 agcacctgtc tctaggaatc gcacgcaacg tactaagacg taagccaggt ccacgctacg
     9781 aagtaactga gccgaatttc acacgccata ccactagacc ttgtggagga tcacgcgaaa
     9841 ttctaaggtt ggacacgcag acacgcaaca ccaatcaaca gttgaacgaa ccacgctaac
     9901 gtggcggcgt ggaacgccag gcacgccagc ctagcaaatg tattaataag acacgccagc
     9961 tgcctacaaa aagcgcttac tgttcacgcg atattagggt ctaaaatgta agaacacgca
    10021 attcggtgag ttagattgga caagcacgct agggatccga agatgttttg gcgccacgcc
    10081 aagttagtca cagggggagc ccctcacgcc agctagcgcg gccgcgactc tagatcataa
    10141 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
    10201 tgaacctgaa acataaaatg aatgcaattg ttgttgttta gtccctccca attcgatatc
    10261 aagcttatcg cgatagatcc taatcaacct ctggattaca aaatttgtga aagattgact
    10321 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
    10381 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
    10441 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
    10501 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
    10561 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
    10621 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
    10681 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
    10741 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
    10801 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
    10861 gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
    10921 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
    10981 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
    11041 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
    11101 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
    11161 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
    11221 caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg gctagatcat
    11281 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
    11341 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
    11401 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
    11461 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
    11521 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
    11581 aaagatgctg aagatcagtt gggtgcacga
//
