LOCUS       sequence-488916-        9976 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Tet- or Dox-inducible expression of TmEnc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9976)
  TITLE     Fei Chen Lab Plasmids 2025
REFERENCE   2  (bases 1 to 9976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKC059_pLVX_TetON_TmEnc
FEATURES             Location/Qualifiers
     source          1..9976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             1119..1752
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1959..2547
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2065..2076
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     2474..2494
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2561..3160)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2644..2664)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3141..3160
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3169..3498)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3183..3318
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(3509..4255)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(4274..4784)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4337..4355)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        4801..5168
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    4809..4827
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4845..4863
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4881..4899
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4917..4935
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4953..4971
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4989..5007
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5025..5043
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      5175..5184
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6171..6305
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    6349..6464
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(6727..6771)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6956..7189
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7686..7811
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7858..8491
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8659..8793
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        8841..8945
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8946..9014,9015..9806)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9164..9183)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtaaa cgctagcaat gtcaaggcct ctcactctct gatattcatt tctttgcaag
     1081 ttataaatac tgaataataa gatgacatga actactactg ctagagattt tccacactga
     1141 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     1201 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     1261 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg
     1321 cttatatgca ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctccctg
     1381 gaaagtcccc agcggaaagt cccttgtagc aagctcgata tcagcagttc ttgaagtact
     1441 ccggatgcag ctctcgggcc acgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     1501 acacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagctggtgt
     1561 tctctccttt attggcctct tctaccttat ctggctcaac tggtactagc ttgtagcacc
     1621 atccaaaggt cagtggatat ctgacccctg gccctggtgt gtagttctgc taatcaggga
     1681 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
     1741 ttagcccttc cagtcccctc ttttctttta aaaagtggct aagatctaca gctgccttgt
     1801 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     1861 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     1921 catgtttttc taggtctcga ctgcagaatt aattccaggc ggggaggcgg cccaaaggga
     1981 gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag
     2041 gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc
     2101 gcgcagaatc caggtggcaa cacaggcgag cagccatgga aaggacgtca gcttccccga
     2161 caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca
     2221 ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag
     2281 ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac
     2341 cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt
     2401 atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa
     2461 agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc
     2521 acaaattttg taatccagag gttgattgtt ccagacgcgt tcaggcaccg ggcttgcggg
     2581 tcatgcacca ggtgcgcggt ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga
     2641 gccgctcgta gaaggggagg ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg
     2701 cgcgctcggc cgcctccact ccggggagca cgacggcgct gcccagaccc ttgccctggt
     2761 ggtcgggcga gacgccgacg gtggccagga accacgcggg ctccttgggc cggtgcggcg
     2821 ccaggaggcc ttccatctgt tgctgcgcgg ccagccggga accgctcaac tcggccatgc
     2881 gcgggccgat ctcggcgaac accgcccccg cttcgacgct ctccggcgtg gtccagaccg
     2941 ccaccgcggc gccgtcgtcc gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga
     3001 ggaagagttc ttgcagctcg gtgacccgct cgatgtggcg gtccgggtcg acggtgtggc
     3061 gcgtggcggg gtagtcggcg aacgcggcgg cgagggtgcg tacggcccgg gggacgtcgt
     3121 cgcgggtggc gaggcgcacc gtgggcttgt actcggtcat ggtcgcgttt tgcaaaagcc
     3181 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc
     3241 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg
     3301 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag
     3361 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc
     3421 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc
     3481 acaccctaac tgacacacat tcgcgcgttt acccggggag catgtcaagg tcaaaatcgt
     3541 caagagcgtc agcaggcagc atatcaaggt caaagtcgtc aagggcatcg gctgggagca
     3601 tgtctaagtc aaaatcgtca agggcgtcgg tcggcccgcc gctttcgcac tttagctgtt
     3661 tctccaggcc acatatgatt agttccaggc cgaaaaggaa ggcaggttcg gctccctgcc
     3721 ggtcgaacag ctcaattgct tgtttcagaa gtgggggcat agaatcggtg gtaggtgtct
     3781 ctctttcctc ttttgctact tgatgctcct gttcctccaa tacgcagccc agtgtaaagt
     3841 ggcccacggc ggacagagcg tacagtgcgt tctccaggga gaagccttgc tgacacagga
     3901 acgcgagctg attttccagg gtttcgtact gtttctctgt tgggcgggtg ccgagatgca
     3961 ctttagcccc gtcgcgatgt gagaggagag cacagcggta tgacttggcg ttgttccgca
     4021 gaaagtcttg ccatgactcg ccttccaggg ggcaggagtg ggtatgatgc ctgtccagca
     4081 tctcgattgg cagggcatcg agcagggccc gcttgttctt cacgtgccag tacagggtag
     4141 gctgctcaac tcccagcttt tgagcgagtt tccttgtcgt caggccttcg ataccgactc
     4201 cattgagtaa ttccagagca gagtttatga ctttgctctt gtccagtcta gacatggtaa
     4261 ttcgatgatc cccctgggga gagaggtcgg tgattcggtc aacgagggag ccgactgccg
     4321 acgtgcgctc cggaggcttg cagaatgcgg aacaccgcgc gggcaggaac agggcccaca
     4381 ctaccgcccc acaccccgcc tcccgcaccg ccccttcccg gccgctgctc tcggcgcgcc
     4441 ctgctgagca gccgctattg gccacagccc atcgcggtcg gcgcgctgcc attgctccct
     4501 ggcgctgtcc gtctgcgagg gtactagtga gacgtgcggc ttccgtttgt cacgtccggc
     4561 acgccgcgaa ccgcaaggaa ccttcccgac ttaggggcgg agcaggaagc gtcgccgggg
     4621 ggcccacaag ggtagcggcg aagatccggg tgacgctgcg aacggacgtg aagaatgtgc
     4681 gagacccagg gtcggcgccg ctgcgtttcc cggaaccacg cccagagcag ccgcgtccct
     4741 gcgcaaaccc agggctgcct tggaaaaggc gcaaccccaa ccccgtggaa ttatcacctc
     4801 gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
     4861 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
     4921 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
     4981 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
     5041 agaacgtata agctttgctt atgtaaacca gggcgcctat aaaagagtgc tgattttttg
     5101 agtaaacttc aattccacaa cacttttgtc ttataccaac tttccgtacc acttcctacc
     5161 ctcgtaaaga attcgccacc atggagtttc tgaaaaggag ttttgcaccc ctgacagaga
     5221 agcagtggca ggaaattgat aaccgggcca gggaaatttt taaaacgcag ctgtacggtc
     5281 ggaaatttgt agacgtggag ggtccatacg ggtgggagta tgcagcgcat cctcttggtg
     5341 aggtggaggt cctctcagac gaaaatgaag tggtgaaatg gggtttgaga aaatcactcc
     5401 cccttattga gctcagagcc acttttaccc tggacctgtg ggaactcgat aatttggaac
     5461 gagggaaacc gaatgttgat cttagcagct tggaggaaac tgtaaggaag gttgcggagt
     5521 ttgaagatga agttatcttt cgaggttgcg aaaagtccgg tgtcaaaggt cttttgagtt
     5581 tcgaggaacg aaagatagag tgtggttcca cgcccaaaga cctgctcgaa gccatcgtac
     5641 gcgcgctgtc tatcttctca aaagacggca ttgaaggccc ctatacactt gtaattaata
     5701 ctgacagatg gattaatttt cttaaagagg aagcagggca ttacccactg gagaaaagag
     5761 tggaagagtg tcttaggggt gggaagataa ttacgacgcc taggatcgaa gatgccctcg
     5821 tcgtaagtga gcgcggtggc gactttaagc tcattctggg ccaggatctg tctattggct
     5881 atgaggatag agaaaaagat gccgttcgac ttttcatcac agaaacattt acatttcagg
     5941 tggttaatcc ggaggcgctt atactgctca agttctaaac cggtgccggc ggatccagac
     6001 cacctcccct gcgagctaag ctggacagcc aatgacgggt aagagagtga catttttcac
     6061 taacctaaga caggagggcc gtcagagcta ctgcctaatc caaagacggg taaaagtgat
     6121 aaaaatgtat cactccaacc taagacaggc gcagcttccg agggatttga gatccagaca
     6181 tgataagata cattgatgag tttggacaaa ccaaaactag aatgcagtga aaaaaatgcc
     6241 ttatttgtga aatttgtgat gctattgcct tatttgtaac cattataagc tgcaataaac
     6301 aagttaagtc gataaactgg atctctgctg tccctgtaat aaacccgaaa attttgaatt
     6361 tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt
     6421 ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaggcgat accgtcgaga
     6481 tccgttcact aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc
     6541 ttcgggcctg tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata
     6601 tccctgccta actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa
     6661 gcctcctact atcattatga ataattttat ataccacagc caatttgtta tgttaaacca
     6721 attccacaaa cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg
     6781 gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc
     6841 ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc
     6901 attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag
     6961 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
     7021 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg
     7081 ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc
     7141 gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc
     7201 tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc
     7261 aatttttact actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc
     7321 tccaggtctg aagatcagcg gccggccgct tgctgtgcgg tggtcttact tttgttttgc
     7381 tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct ttgatgcaca
     7441 caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc tgtctgaagg
     7501 gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa caggccagga
     7561 ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa tttatatttt
     7621 ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct cccccgctta
     7681 atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa aatttttggc
     7741 gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg
     7801 cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg gcgccactgc
     7861 tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca gagtcacaca
     7921 acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc ttaagcagtg
     7981 ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag agagacccag
     8041 tacaggcaaa aagcagctgc ttatatgcag gatctgaggg ctcgccactc cccagtcccg
     8101 cccaggccac gcctccctgg aaagtcccca gcggaaagtc ccttgtagca agctcgatat
     8161 cagcagttct tgaagtactc cggatgcagc tctcgggcca cgtgatgaaa tgctaggcgg
     8221 ctgtcaaacc tccactctaa cacttctctc tccgggtcat ccatcccatg caggctcaca
     8281 gggtgtaaca agctggtgtt ctctccttta ttggcctctt ctaccttatc tggctcaact
     8341 ggtactagct tgtagcacca tccaaaggtc agtggatatc tgacccctgg ccctggtgtg
     8401 tagttctgct aatcagggaa gtagccttgt gtgtggtaga tccacagatc aaggatatct
     8461 tgtcttcttt gggagtgaat tagcccttcc aactactaag tttgtagtac atatttaaca
     8521 aatacaattt ctttaaaatg aaaataattc agaggaatca caggtttaga gtaaatgaaa
     8581 ccacaggtaa ttggcagtgg taatagggta tggggtggga agtttgggat gattttggtt
     8641 agcttgagtt atccagttga tccagacatg ataagataca ttgatgagtt tggacaaacc
     8701 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     8761 tttgtaacca ttataagctg caataaacaa gttgatctcc cgatccgtcg acgtcaggtg
     8821 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     8881 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     8941 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     9001 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     9061 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     9121 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     9181 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     9241 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     9301 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     9361 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     9421 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     9481 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     9541 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     9601 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     9661 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     9721 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     9781 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     9841 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     9901 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     9961 agatcaaagg atcttc
//
