LOCUS       sequence-488925-       11423 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Tet- or Dox-inducible expression of MVP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11423)
  TITLE     Fei Chen Lab Plasmids 2025
REFERENCE   2  (bases 1 to 11423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKC104_pLenti_TetON_MVP
FEATURES             Location/Qualifiers
     source          1..11423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1000..1021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1036..1041,1042..1059,1060..1066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1074..1090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1079..1101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1098..1114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1098..1114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1164..1183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1375..2008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2055..2180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2677..2910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3095..3139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3432..3549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3620..3638
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3656..3674
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3692..3710
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3728..3746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3764..3782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3800..3818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3836..3854
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3899..3923
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4048..4057
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6742..6759
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6800..6819)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6865..6920
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6919..6938)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        7124..7335
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             7348..7616
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      7651..7660
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7657..8367
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             8374..8427
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8428..9174
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    9187..9775
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9240..9260)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9658..9669)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9850..10083
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10202..10220)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10288..10392
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10393..10461,10462..11253)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10611..10630)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
      841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      901 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
      961 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1021 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1081 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
     1141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     1261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     1321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     1381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     1441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     1501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     1561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     1621 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     1681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     1741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     1801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     1861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     2821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     2881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3361 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca aatggcagta
     3421 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3481 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3541 caaaattttc gggtttatta cagggacagc agagatccag tttggactag accggtggcc
     3601 tccacggcca ctagattact ccctatcagt gatagagaac gtatgaagag tttactccct
     3661 atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg
     3721 agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga
     3781 gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag tttactccct
     3841 atcagtgata gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag
     3901 ctcgtttagt gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatacca
     3961 actttccgta ccacttccta ccctcgtaaa gaagttccta ttccgaagtt cctattcttc
     4021 aaaaggtata ggaacttcgc tcgagccgcc accatggcaa ctgaagagtt catcatccgc
     4081 atccccccat accactatat ccatgtgctg gaccagaaca gcaacgtgtc ccgtgtggag
     4141 gtcgggccaa agacctacat ccggcaggac aatgagaggg tactgtttgc ccccatgcgc
     4201 atggtgaccg tccccccacg tcactactgc acagtggcca accctgtgtc tcgggatgcc
     4261 cagggcttgg tgctgtttga tgtcacaggg caagttcggc ttcgccacgc tgacctcgag
     4321 atccggctgg cccaggaccc cttccccctg tacccagggg aggtgctgga aaaggacatc
     4381 acacccctgc aggtggttct gcccaacact gccctccatc taaaggcgct gcttgatttt
     4441 gaggataaag atggagacaa ggtggtggca ggagatgagt ggcttttcga gggacctggc
     4501 acgtacatcc cccggaagga agtggaggtc gtggagatca ttcaggccac catcatcagg
     4561 cagaaccagg ctctgcggct cagggcccgc aaggagtgct gggaccggga cggcaaggag
     4621 agggtgacag gggaagaatg gctggtcacc acagtagggg cgtacctccc agcggtgttt
     4681 gaggaggttc tggatttggt ggacgccgtc atccttacgg aaaagacagc cctgcacctc
     4741 cgggctcggc ggaacttccg ggacttcagg ggagtgtccc gccgcactgg ggaggagtgg
     4801 ctggtaacag tgcaggacac agaggcccac gtgccagatg tccacgagga ggtgctgggg
     4861 gttgtgccca tcaccaccct gggcccccac aactactgcg tgattctcga ccctgtcgga
     4921 ccggatggca agaatcagct ggggcagaag cgcgtggtca agggagagaa gtcttttttc
     4981 ctccagccag gagagcagct ggaacaaggc atccaggatg tgtatgtgct gtcggagcag
     5041 caggggctgc tgctgagggc cctgcagccc ctggaggagg gggaggatga ggagaaggtc
     5101 tcacaccagg ctggggacca ctggctcatc cgcggacccc tggagtatgt gccatctgcc
     5161 aaagtggagg tggtggagga gcgccaggcc atccctctag acgagaacga gggcatctat
     5221 gtgcaggatg tcaagaccgg aaaggtgcgc gctgtgattg gaagcaccta catgctgacc
     5281 caggacgaag tcctgtggga gaaagagctg cctcccgggg tggaggagct gctgaacaag
     5341 gggcaggacc ctctggcaga caggggtgag aaggacacag ctaagagcct ccagcccttg
     5401 gcgccccgga acaagacccg tgtggtcagc taccgcgtgc cccacaacgc tgcggtgcag
     5461 gtgtacgact accgagagaa gcgagcccgc gtggtcttcg ggcctgagct ggtgtcgctg
     5521 ggtcctgagg agcagttcac agtgttgtcc ctctcagctg ggcggcccaa gcgtccccat
     5581 gcccgccgtg cgctctgcct gctgctgggg cctgacttct tcacagacgt catcaccatc
     5641 gaaacggcgg atcatgccag gctgcaactg cagctggcct acaactggca ctttgaggtg
     5701 aatgaccgga aggaccccca agagacggcc aagctctttt cagtgccaga ctttgtaggt
     5761 gatgcctgca aagccatcgc atcccgggtg cggggggccg tggcctctgt cactttcgat
     5821 gacttccata agaactcagc ccgcatcatt cgcactgctg tctttggctt tgagacctcg
     5881 gaagcgaagg gccccgatgg catggccctg cccaggcccc gggaccaggc tgtcttcccc
     5941 caaaacgggc tggtggtcag cagtgtggac gtgcagtcag tggagcctgt ggatcagagg
     6001 acccgggacg ccctgcaacg cagcgtccag ctggccatcg agatcaccac caactcccag
     6061 gaagcggcgg ccaagcatga ggctcagaga ctggagcagg aagcccgcgg ccggcttgag
     6121 cggcagaaga tcctggacca gtcagaagcc gagaaagctc gcaaggaact tttggagctg
     6181 gaggctctga gcatggccgt ggagagcacc gggactgcca aggcggaggc cgagtcccgt
     6241 gcggaggcag cccggattga gggagaaggg tccgtgctgc aggccaagct aaaagcacag
     6301 gccttggcca ttgaaacgga ggctgagctc cagagggtcc agaaggtccg agagctggaa
     6361 ctggtctatg cccgggccca gctggagctg gaggtgagca aggctcagca gctggctgag
     6421 gtggaggtga agaagttcaa gcagatgaca gaggccatag gccccagcac catcagggac
     6481 cttgctgtgg ctgggcctga gatgcaggta aaactgctcc agtccctggg cctgaaatca
     6541 accctcatca ccgatggctc cactcccatc aacctcttca acacagcctt tgggctgctg
     6601 gggatggggc ccgagggtca gcccctgggc agaagggtgg ccagtgggcc cagccctggg
     6661 gaggggatat ccccccagtc tgctcaggcc cctcaagctc ctggagacaa ccacgtggtg
     6721 cctgtactgc ggggtggtag ccatcatcat caccatcact aaggatccac cggatctaga
     6781 taactgagcg gccgcctaat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     6841 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     6901 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     6961 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     7021 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttac
     7081 atatgactag taaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     7141 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     7201 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     7261 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     7321 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     7381 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     7441 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     7501 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     7561 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     7621 ctgtgaccgg cgcctacacc tgcagccaac gccaccatgg tgtctaaggg cgaagagctg
     7681 attaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc
     7741 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     7801 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac
     7861 ggcagcagaa ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc
     7921 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
     7981 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
     8041 aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccaacacc
     8101 gagatgctgt accccgctga cggcggcctg gaaggcagaa gcgacatggc cctgaagctc
     8161 gtgggcgggg gccacctgat ctgcaacttc aagaccacat acagatccaa gaaacccgct
     8221 aagaacctca agatgcccgg cgtctactat gtggaccaca gactggaaag aatcaaggag
     8281 gccgacaaag agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc
     8341 cctagcaaac tggggcacaa acttaatgct agcgagggca gaggaagtct gctaacatgc
     8401 ggtgacgtcg aggagaatcc tggcccaatg tctagactgg acaagagcaa agtcataaac
     8461 tctgctctgg aattactcaa tggagtcggt atcgaaggcc tgacgacaag gaaactcgct
     8521 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
     8581 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccactcctg ccccctggaa
     8641 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc
     8701 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
     8761 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
     8821 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
     8881 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaaa
     8941 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
     9001 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc
     9061 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat
     9121 atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaagtcgac
     9181 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     9241 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     9301 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     9361 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     9421 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     9481 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     9541 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     9601 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     9661 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     9721 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
     9781 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     9841 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     9901 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     9961 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    10021 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    10081 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
    10141 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
    10201 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
    10261 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    10321 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    10381 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    10441 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    10501 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    10561 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    10621 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    10681 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    10741 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    10801 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    10861 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    10921 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    10981 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    11041 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    11101 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    11161 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    11221 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    11281 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    11341 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    11401 gtagaaaaga tcaaaggatc ttc
//
