LOCUS       sequence-488926-       13622 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Tet- or Dox-repressible expression of MVP and PABPC1 fused to INT 
            (human PARP4 fragment).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13622)
  TITLE     Fei Chen Lab Plasmids 2025
REFERENCE   2  (bases 1 to 13622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKC117_pLenti_TetOff_MVP_IRES_PABPC1_INT
FEATURES             Location/Qualifiers
     source          1..13622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1000..1021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1036..1041,1042..1059,1060..1066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1074..1090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1079..1101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1098..1114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1098..1114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1164..1183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1375..2008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2055..2180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2677..2910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3095..3139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3432..3549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3620..3638
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3656..3674
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3692..3710
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3728..3746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3764..3782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3800..3818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3836..3854
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3899..3923
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      4048..4057
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6742..6759
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    join(6764..7338,7339..7341,7342..7350)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6931..6948)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7158..7177
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      7360..7369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8999..9018)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9064..9119
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9118..9137)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        9323..9534
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             9547..9815
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      9850..9859
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9856..10566
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             10573..10626
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(10627..11244,11245..11250,11251..11286,11287..11289,
                     11290..11325,11326..11328,11329..11364,11365..11373)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIECLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    11386..11974
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11439..11459)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11857..11868)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             12049..12282
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(12401..12419)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12487..12591
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12592..12660,12661..13452)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12810..12829)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
      841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      901 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
      961 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1021 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1081 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
     1141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     1261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     1321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     1381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     1441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     1501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     1561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     1621 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     1681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     1741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     1801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     1861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     2821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     2881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3361 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca aatggcagta
     3421 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3481 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3541 caaaattttc gggtttatta cagggacagc agagatccag tttggactag accggtggcc
     3601 tccacggcca ctagattact ccctatcagt gatagagaac gtatgaagag tttactccct
     3661 atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg
     3721 agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga
     3781 gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag tttactccct
     3841 atcagtgata gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag
     3901 ctcgtttagt gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatacca
     3961 actttccgta ccacttccta ccctcgtaaa gaagttccta ttccgaagtt cctattcttc
     4021 aaaaggtata ggaacttcgc tcgagccgcc accatggcaa ctgaagagtt catcatccgc
     4081 atccccccat accactatat ccatgtgctg gaccagaaca gcaacgtgtc ccgtgtggag
     4141 gtcgggccaa agacctacat ccggcaggac aatgagaggg tactgtttgc ccccatgcgc
     4201 atggtgaccg tccccccacg tcactactgc acagtggcca accctgtgtc tcgggatgcc
     4261 cagggcttgg tgctgtttga tgtcacaggg caagttcggc ttcgccacgc tgacctcgag
     4321 atccggctgg cccaggaccc cttccccctg tacccagggg aggtgctgga aaaggacatc
     4381 acacccctgc aggtggttct gcccaacact gccctccatc taaaggcgct gcttgatttt
     4441 gaggataaag atggagacaa ggtggtggca ggagatgagt ggcttttcga gggacctggc
     4501 acgtacatcc cccggaagga agtggaggtc gtggagatca ttcaggccac catcatcagg
     4561 cagaaccagg ctctgcggct cagggcccgc aaggagtgct gggaccggga cggcaaggag
     4621 agggtgacag gggaagaatg gctggtcacc acagtagggg cgtacctccc agcggtgttt
     4681 gaggaggttc tggatttggt ggacgccgtc atccttacgg aaaagacagc cctgcacctc
     4741 cgggctcggc ggaacttccg ggacttcagg ggagtgtccc gccgcactgg ggaggagtgg
     4801 ctggtaacag tgcaggacac agaggcccac gtgccagatg tccacgagga ggtgctgggg
     4861 gttgtgccca tcaccaccct gggcccccac aactactgcg tgattctcga ccctgtcgga
     4921 ccggatggca agaatcagct ggggcagaag cgcgtggtca agggagagaa gtcttttttc
     4981 ctccagccag gagagcagct ggaacaaggc atccaggatg tgtatgtgct gtcggagcag
     5041 caggggctgc tgctgagggc cctgcagccc ctggaggagg gggaggatga ggagaaggtc
     5101 tcacaccagg ctggggacca ctggctcatc cgcggacccc tggagtatgt gccatctgcc
     5161 aaagtggagg tggtggagga gcgccaggcc atccctctag acgagaacga gggcatctat
     5221 gtgcaggatg tcaagaccgg aaaggtgcgc gctgtgattg gaagcaccta catgctgacc
     5281 caggacgaag tcctgtggga gaaagagctg cctcccgggg tggaggagct gctgaacaag
     5341 gggcaggacc ctctggcaga caggggtgag aaggacacag ctaagagcct ccagcccttg
     5401 gcgccccgga acaagacccg tgtggtcagc taccgcgtgc cccacaacgc tgcggtgcag
     5461 gtgtacgact accgagagaa gcgagcccgc gtggtcttcg ggcctgagct ggtgtcgctg
     5521 ggtcctgagg agcagttcac agtgttgtcc ctctcagctg ggcggcccaa gcgtccccat
     5581 gcccgccgtg cgctctgcct gctgctgggg cctgacttct tcacagacgt catcaccatc
     5641 gaaacggcgg atcatgccag gctgcaactg cagctggcct acaactggca ctttgaggtg
     5701 aatgaccgga aggaccccca agagacggcc aagctctttt cagtgccaga ctttgtaggt
     5761 gatgcctgca aagccatcgc atcccgggtg cggggggccg tggcctctgt cactttcgat
     5821 gacttccata agaactcagc ccgcatcatt cgcactgctg tctttggctt tgagacctcg
     5881 gaagcgaagg gccccgatgg catggccctg cccaggcccc gggaccaggc tgtcttcccc
     5941 caaaacgggc tggtggtcag cagtgtggac gtgcagtcag tggagcctgt ggatcagagg
     6001 acccgggacg ccctgcaacg cagcgtccag ctggccatcg agatcaccac caactcccag
     6061 gaagcggcgg ccaagcatga ggctcagaga ctggagcagg aagcccgcgg ccggcttgag
     6121 cggcagaaga tcctggacca gtcagaagcc gagaaagctc gcaaggaact tttggagctg
     6181 gaggctctga gcatggccgt ggagagcacc gggactgcca aggcggaggc cgagtcccgt
     6241 gcggaggcag cccggattga gggagaaggg tccgtgctgc aggccaagct aaaagcacag
     6301 gccttggcca ttgaaacgga ggctgagctc cagagggtcc agaaggtccg agagctggaa
     6361 ctggtctatg cccgggccca gctggagctg gaggtgagca aggctcagca gctggctgag
     6421 gtggaggtga agaagttcaa gcagatgaca gaggccatag gccccagcac catcagggac
     6481 cttgctgtgg ctgggcctga gatgcaggta aaactgctcc agtccctggg cctgaaatca
     6541 accctcatca ccgatggctc cactcccatc aacctcttca acacagcctt tgggctgctg
     6601 gggatggggc ccgagggtca gcccctgggc agaagggtgg ccagtgggcc cagccctggg
     6661 gaggggatat ccccccagtc tgctcaggcc cctcaagctc ctggagacaa ccacgtggtg
     6721 cctgtactgc ggggtggtag ccatcatcat caccatcact aagcccctct ccctcccccc
     6781 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt
     6841 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct
     6901 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga
     6961 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga
     7021 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac
     7081 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag
     7141 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc
     7201 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtacacatgc tttacatgtg
     7261 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     7321 gaaaaacacg atgataatat ggccacaacc ctggaattcg ccaccatggc ctcgctctac
     7381 gtgggggacc tccaccccga cgtgaccgag gcgatgctct acgagaagtt cagcccggcc
     7441 gggcccatcc tctccatccg ggtctgcagg gacatgatca cccgccgctc cttgggctac
     7501 gcgtatgtga acttccagca gccggcggac gcggagcgtg ctttggacac catgaatttt
     7561 gatgttataa agggcaagcc agtacgcatc atgtggtctc agcgtgatcc atcacttcgc
     7621 aaaagtggag taggcaacat attcattaaa aatctggaca aatccattga taataaagca
     7681 ctgtatgata cattttctgc ttttggtaac atcctttcat gtaaggtggt ttgtgatgaa
     7741 aatggttcca agggctatgg atttgtacac tttgagacgc aggaagcagc tgaaagagct
     7801 attgaaaaaa tgaatggaat gctcctaaat gatcgcaaag tatttgttgg acgatttaag
     7861 tctcgtaaag aacgagaagc tgaacttgga gctagggcaa aagaattcac caatgtttac
     7921 atcaagaatt ttggagaaga catggatgat gagcgcctta aggatctctt tggcaagttt
     7981 gggcctgcct taagtgtgaa agtaatgact gatgaaagtg gaaaatccaa aggatttgga
     8041 tttgtaagct ttgaaaggca tgaagatgca cagaaagctg tggatgagat gaacggaaag
     8101 gagctcaatg gaaaacaaat ttatgttggt cgagctcaga aaaaggtgga acggcagacg
     8161 gaacttaagc gcaaatttga acagatgaaa caagatagga tcaccagata ccagggtgtt
     8221 aatctttatg tgaaaaatct tgatgatggt attgatgatg aacgtctccg gaaagagttt
     8281 tctccatttg gtacaatcac tagtgcaaag gttatgatgg agggtggtcg cagcaaaggg
     8341 tttggttttg tatgtttctc ctccccagaa gaagccacta aagcagttac agaaatgaac
     8401 ggtagaattg tggccacaaa gccattgtat gtagctttag ctcagcgcaa agaagagcgc
     8461 ggtggtagcg tgtgcatcca gcactggcaa gatgccgttc cttggacgga attgctgtct
     8521 ctccagacgg aggatggttt ctggaagctc acgcccgaac tcggactcat attgaacctt
     8581 aatacaaatg ggctccactc ctttctgaag cagaaaggca tccagagtct cggtgttaag
     8641 ggccgggagt gccttctcga cctcatagcc acgatgctcg tgttgcaatt tatccgcact
     8701 aggctggaaa aagagggtat cgtattcaaa agtctgatga aaatggacga cgctagtata
     8761 agtagaaaca tcccctgggc ttttgaggcc atcaagcagg cgagtgagtg ggtcaggcga
     8821 acagagggtc agtacccaag catatgtccc cgactggagt tggggaacga ctgggatagc
     8881 gcaaccaagc agctcttggg tctgcaacca atttcaacag tgtcaccact gcatcgagtc
     8941 ctgcattact cccagggcta aggatccacc ggatctagat aactgagcgg ccgcctaatc
     9001 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     9061 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     9121 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     9181 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     9241 atcagtatat gaaacagccc cctgctgtcc attccttaca tatgactagt aaggatctgc
     9301 gatcgctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     9361 gggggagggg tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa
     9421 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     9481 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag
     9541 cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca
     9601 cgccggttga gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc
     9661 gtctaggtaa gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc
     9721 ctacctagac tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc
     9781 tttgtttcgt tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacacct
     9841 gcagccaacg ccaccatggt gtctaagggc gaagagctga ttaaggagaa catgcacatg
     9901 aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa
     9961 ggcaagccct acgagggcac ccagaccatg agaatcaagg tggtcgaggg cggccctctc
    10021 cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcagaac cttcatcaac
    10081 cacacccagg gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag
    10141 agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcctccag
    10201 gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcccatc caacggccct
    10261 gtgatgcaga agaaaacact cggctgggag gccaacaccg agatgctgta ccccgctgac
    10321 ggcggcctgg aaggcagaag cgacatggcc ctgaagctcg tgggcggggg ccacctgatc
    10381 tgcaacttca agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc
    10441 gtctactatg tggaccacag actggaaaga atcaaggagg ccgacaaaga gacctacgtc
    10501 gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact ggggcacaaa
    10561 cttaatgcta gcgagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct
    10621 ggcccaatgt ctagactgga caagagcaaa gtcataaact ctgctctgga attactcaat
    10681 gaagtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag
    10741 cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct ggcaatcgag
    10801 tgtctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt
    10861 ctgcggaaca acgccaagtc attccgctgt gctctcctct cacatcgcga cggggctaaa
    10921 gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg
    10981 ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc
    11041 cactttacac tgggctgcgt attggaggat caggagcatc aagtagcaaa agaggaaaga
    11101 gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac
    11161 catcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag
    11221 aaacagctaa agtgcgaaag cggcgggccg gccgacgccc ttgacgattt tgacttagac
    11281 atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt
    11341 gacgattttg accttgacat gctccccgga tgagtcgacg atcctaatca acctctggat
    11401 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
    11461 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
    11521 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
    11581 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
    11641 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
    11701 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
    11761 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
    11821 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
    11881 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
    11941 acgagtcgga tctccctttg ggccgcctcc ccgcctgaga tcctttaaga ccaatgactt
    12001 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
    12061 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
    12121 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
    12181 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
    12241 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
    12301 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggcccgggt
    12361 taattaagga aagggctaga tcattcttga agacgaaagg gcctcgtgat acgcctattt
    12421 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
    12481 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
    12541 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
    12601 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
    12661 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
    12721 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    12781 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
    12841 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    12901 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    12961 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    13021 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    13081 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
    13141 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    13201 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    13261 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    13321 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    13381 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    13441 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    13501 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    13561 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    13621 tc
//
