LOCUS       sequence-488928-        9563 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Tet- or Dox-inducible expression of QtEnc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9563)
  TITLE     Fei Chen Lab Plasmids 2025
REFERENCE   2  (bases 1 to 9563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKC052_pLenti_TetON_QtEnc
FEATURES             Location/Qualifiers
     source          1..9563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1000..1021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1036..1041,1042..1059,1060..1066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1074..1090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1079..1101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1098..1114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1098..1114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1164..1183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1375..2008
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2055..2180
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2677..2910
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3095..3139
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3432..3549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3620..3638
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3656..3674
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3692..3710
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3728..3746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3764..3782
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3800..3818
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3836..3854
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3899..3923
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(4940..4959)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5005..5060
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5059..5078)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     promoter        5264..5475
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             5488..5756
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      5791..5800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5797..6507
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             6514..6567
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6568..7314
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7327..7915
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7380..7400)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7798..7809)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7990..8223
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8342..8360)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8428..8532
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8533..8601,8602..9393)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8751..8770)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
      841 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      901 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
      961 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1021 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1081 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
     1141 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     1201 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
     1261 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
     1321 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagttggaag
     1381 ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac cacacacaag
     1441 gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat ccactgacct
     1501 ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag gccaataaag
     1561 gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac ccggagagag
     1621 aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc cgagagctgc
     1681 atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt tccgctgggg
     1741 actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc agatcctgca
     1801 tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     1861 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     1921 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     1981 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     2041 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     2101 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     2161 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     2221 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     2281 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     2341 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     2401 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     2461 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     2521 ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     2581 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     2641 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     2701 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     2761 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     2821 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     2881 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2941 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     3001 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     3061 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     3121 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     3181 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     3241 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     3301 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     3361 cagatccatt cgattagtga acggatctcg acggtatcgc cgaattcaca aatggcagta
     3421 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3481 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3541 caaaattttc gggtttatta cagggacagc agagatccag tttggactag accggtggcc
     3601 tccacggcca ctagattact ccctatcagt gatagagaac gtatgaagag tttactccct
     3661 atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg
     3721 agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga
     3781 gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag tttactccct
     3841 atcagtgata gagaacgtat aagctttagg cgtgtacggt gggcgcctat aaaagcagag
     3901 ctcgtttagt gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatacca
     3961 actttccgta ccacttccta ccctcgtaaa gaagttccta ttccgaagtt cctattcttc
     4021 aaaaggtata ggaacttcgc tcgagccgcc accatgaata agtctcagct ctaccccgat
     4081 agcccgctca cggaccagga ttttaatcaa ctggatcaaa ccgtaatcga agctgcacga
     4141 cgccaacttg ttggaaggag atttatcgag ctgtatggtc ctttgggacg aggaatgcag
     4201 tctgtgttta atgacatatt catggagtcc catgaggcaa agatggattt ccaaggaagc
     4261 ttcgatacag aagtagagag ttcccggcgg gtcaactaca ccattcctat gctttacaaa
     4321 gatttcgttc tttactggag agatctcgag cagtccaaag cgctggatat accgatcgac
     4381 tttagcgtgg cagccaacgc agctcgagat gttgcttttc ttgaggatca gatgatcttt
     4441 cacggaagta aggagttcga tattccgggg ttgatgaatg tgaaagggag gcttacgcac
     4501 ctcataggta attggtacga aagtggtaac gcattccaag atatagttga ggcgcggaac
     4561 aaactcttgg agatgaacca taatggacct tatgctcttg tgctgtctcc agagctttac
     4621 tccttgttgc atagggtcca caaagacacc aacgtcctcg aaatcgagca cgttcgggag
     4681 ctcatcactg ctggtgtctt ccaatcccca gtacttaaag gcaaaagcgg ggtgatagtt
     4741 aatacgggaa ggaataacct tgaccttgcc atcagtgaag attttgagac ggcttatctt
     4801 ggcgaagaag ggatgaatca cccctttaga gtttacgaaa ctgtggtcct tcggattaaa
     4861 cggcctgcgg ctatctgcac gctcatcgat cctgaggagt aaggatccac cggatctaga
     4921 taactgagcg gccgcctaat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     4981 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     5041 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     5101 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     5161 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttac
     5221 atatgactag taaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg
     5281 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg
     5341 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     5401 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     5461 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc
     5521 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt
     5581 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt
     5641 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc
     5701 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag
     5761 ctgtgaccgg cgcctacacc tgcagccaac gccaccatgg tgtctaaggg cgaagagctg
     5821 attaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc
     5881 aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag
     5941 gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac
     6001 ggcagcagaa ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc
     6061 cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct
     6121 acccaggaca ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg
     6181 aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccaacacc
     6241 gagatgctgt accccgctga cggcggcctg gaaggcagaa gcgacatggc cctgaagctc
     6301 gtgggcgggg gccacctgat ctgcaacttc aagaccacat acagatccaa gaaacccgct
     6361 aagaacctca agatgcccgg cgtctactat gtggaccaca gactggaaag aatcaaggag
     6421 gccgacaaag agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc
     6481 cctagcaaac tggggcacaa acttaatgct agcgagggca gaggaagtct gctaacatgc
     6541 ggtgacgtcg aggagaatcc tggcccaatg tctagactgg acaagagcaa agtcataaac
     6601 tctgctctgg aattactcaa tggagtcggt atcgaaggcc tgacgacaag gaaactcgct
     6661 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
     6721 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccactcctg ccccctggaa
     6781 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc
     6841 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
     6901 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
     6961 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
     7021 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaaa
     7081 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
     7141 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc
     7201 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat
     7261 atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaagtcgac
     7321 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     7381 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     7441 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7501 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7561 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7621 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7681 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     7741 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7801 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7861 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
     7921 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7981 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     8041 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8101 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8161 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8221 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     8281 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
     8341 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     8401 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     8461 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     8521 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     8581 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     8641 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     8701 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     8761 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     8821 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     8881 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     8941 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     9001 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     9061 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     9121 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     9181 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     9241 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     9301 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     9361 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     9421 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     9481 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     9541 gtagaaaaga tcaaaggatc ttc
//
