LOCUS       sequence-488948-        8248 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Recombinant protein expression of PCMTD1(∆ESB-ASB9).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Pang EZ, Zhao B, Flowers C, Oroudjeva E, Winter JB, Pandey V, Sawaya
            MR, Wohlschlegel J, Loo JA, Rodriguez JA, Clarke SG
  TITLE     Structural basis for L-isoaspartyl-containing protein recognition by
            the PCMTD1 cullin-RING E3 ubiquitin ligase.
  JOURNAL   bioRxiv [Preprint]. 2025 May 21:2025.05.21.654933. doi: 
            10.1101/2025.05.21.654933.
  PUBMED    40475564
REFERENCE   2  (bases 1 to 8248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2025 May 21:2025.05.21.654933. doi: 
            10.1101/2025.05.21.654933."
COMMENT     Sequence Label: pMAPLe4 PCMTD1 PCMTD1(∆ESB-ASB9)
FEATURES             Location/Qualifiers
     source          1..8248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(892..910)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      906..953
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      990..1445
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1077..1096)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1287..1308
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(1537..2352)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1537..2352)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2260..2279
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2344..2363
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2474..3062
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2963..2982
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3216..3233
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3248..3387
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3313..3332)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3473..3495
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3489..3680)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     4423..4441
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        join(4462..4480,4481..4486,4487..4503,4504..4509,
                     4510..4535)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    4462..4480
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4487..4505
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             4545..4553
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4559..5269
                     /codon_start=1
                     /product="tobacco vein mottling virus NIa protease 
                     (Nallamsetty et al., 2004)"
                     /label=TVMV protease
                     /translation="MSKALLKGVRDFNPISACVCLLENSSDGHSERLFGIGFGPYIIAN
                     QHLFRRNNGELTIKTMHGEFKVKNSTQLQMKPVEGRDIIVIKMAKDFPPFPQKLKFRQP
                     TIKDRVCMVSTNFQQKSVSSLVSESSHIVHKEDTSFWQHWITTKDGQCGSPLVSIIDGN
                     ILGIHSLTHTTNGSNYFVEFPEKFVATYLDAADGWCKNWKFNADKISWGSFTLVEDAPE
                     DDFMAKKTVAAIMD"
     terminator      5302..5388
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(5514..6596)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     6558..6577
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(6597..6674)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     6880..6899
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     6983..7002
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6983..7001
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7002..7026
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7041..7063
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             7070..8170
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      8027..8036
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     8144..8167
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             8180..8200
                     /codon_start=1
                     /product="tobacco vein mottling virus (TVMV) NIa protease 
                     recognition and cleavage site
                     "
                     /label=TVMV site
                     /translation="ETVRFQS"
     CDS             8201..8218
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8228..8248
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgggtggtg cagtgagtgc aggagaggat aacgacgatc ttattgataa cttgaaagag
       61 gcccagtata tacgtacgga acgggtagag caggcttttc gtgcgataga ccgcggcgat
      121 tactatcttg aaggatatcg tgataacgcc tataaagacc tggcgtggaa gcatggaaat
      181 attcatcttt ccgcaccttg tatctatagt gaagttatgg aagccctcaa gcttcaaccg
      241 ggattgagtt tcttaaacct gggatctggc actggttatc tgtcaacgat ggtaggcctt
      301 attcttggac ctttcggtat caaccatggg atcgaattac attctgatgt tgtggaatat
      361 gccaaagaaa aactggagtc atttattaag aattcggata gcttcgataa atttgaattt
      421 tgtgaaccgg ccttcgtggt agggaactgc ttacaaatcg ccagcgattc tcatcaatat
      481 gatcgtatat attgtggcgc aggtgttcag aaggatcatg aaaactatat gaaaattttg
      541 ctgaaagtcg gaggcatttt ggtaatgcca atagaggacc agttaacgca aataatgcgc
      601 acaggacaga acacctggga aagcaagaat attctggcag tatcgtttgc gccgctggtc
      661 caaccgtcta aaaatgataa cggaaaaccg gatagtgtag ggctcccgcc ctgcgcggtt
      721 cgtaacctgc aggatctcgc acgtatttat atccgaaaat gcttcggaat acagcagcac
      781 cataaaatca cgaaattacc gctgccggag agcctgaaag cgtatctgac ctattttcga
      841 gataagtaac tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
      901 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
      961 gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     1021 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     1081 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     1141 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     1201 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     1261 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     1321 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     1381 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     1441 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     1501 atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa
     1561 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     1621 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
     1681 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     1741 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     1801 tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     1861 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     1921 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
     1981 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     2041 tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     2101 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
     2161 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     2221 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     2281 atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg
     2341 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     2401 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2461 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2521 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2581 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2641 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2701 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2761 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2821 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2881 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2941 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3001 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3061 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3121 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3181 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3241 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcaatg
     3301 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     3361 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     3421 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     3481 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc
     3541 tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg
     3601 ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg
     3661 gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta
     3721 atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc
     3781 cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga
     3841 aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt
     3901 agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc
     3961 gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca
     4021 gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa
     4081 ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc
     4141 acccgtgggg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg
     4201 ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg
     4261 ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc
     4321 ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc taagatagtc
     4381 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     4441 ccctttcgtc ttcacctcga gtccctatca gtgatagaga ttgacatccc tatcagtgat
     4501 agagatactg agcacatcag caggacgcac tgaccgaatt gatttaagga ggtaacatat
     4561 gtctaaagct ttgctgaagg gcgtgcgcga ttttaatccg atctctgctt gcgtatgcct
     4621 gctggaaaac tcctcggatg gtcatagtga acgtctgttt ggcattggtt ttggcccgta
     4681 tatcattgcc aaccagcatc tgtttcgtcg taacaatggc gaactgacca tcaaaaccat
     4741 gcatggtgaa ttcaaagtca aaaactctac ccagctgcag atgaaaccgg ttgaaggccg
     4801 tgacattatc gttatcaaaa tggctaaaga cttcccgccg ttcccgcaga aactgaaatt
     4861 ccgtcagccg accatcaaag atcgtgtgtg catggtgtcc accaactttc agcagaaaag
     4921 cgtctcgagc ctggtgtctg aatcctctca cattgtgcat aaagaagaca cttctttctg
     4981 gcagcactgg atcaccacta aagatggcca gtgtggcagc ccactagttt ccatcattga
     5041 tggcaacatt ctgggcatcc acagcctgac tcataccacc aacggtagca actacttcgt
     5101 ggaatttccg gaaaaattcg tggcgactta tctagatgcc gcggatggtt ggtgcaaaaa
     5161 ctggaaattc aacgcggata aaatcagctg gggttccttt accctggttg aagatgcgcc
     5221 ggaagatgac ttcatggcca aaaaaactgt tgccgccatc atggactaat aaagatccca
     5281 tggtacgcgt gctagaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     5341 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag
     5401 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
     5461 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc
     5521 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     5581 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
     5641 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     5701 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
     5761 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     5821 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     5881 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
     5941 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
     6001 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
     6061 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
     6121 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
     6181 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
     6241 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
     6301 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
     6361 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
     6421 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
     6481 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
     6541 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
     6601 accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc
     6661 cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca
     6721 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
     6781 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc
     6841 gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc
     6901 gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat
     6961 cgagatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac
     7021 aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacca tgaaaatcga
     7081 agaaggtaaa ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt
     7141 cggtaagaaa ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact
     7201 ggaagagaaa ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc
     7261 acacgaccgc tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa
     7321 agcgttccag gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct
     7381 gattgcttac ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc
     7441 gaacccgcca aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg
     7501 taagagcgcg ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc
     7561 tgacgggggt tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt
     7621 ggataacgct ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca
     7681 catgaatgca gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc
     7741 gatgaccatc aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg
     7801 tgtaacggta ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag
     7861 cgcaggtatt aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta
     7921 tctgctgact gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc
     7981 gctgaagtct tacgaggaag agttggcgaa agatccacgt attgccgcca ccatggaaaa
     8041 cgcccagaaa ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt
     8101 gcgtactgcg gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga
     8161 cgcgcagact aatggctcag aaacagttcg gttccagtct catcaccatc accatcactc
     8221 gagctcagag aacctgtatt tccagagt
//
