LOCUS       sequence-488949-        3245 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Entry vector for Gateway with USP13 (AA776-1102).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Nie L, Wang C, Liu X, Teng H, Li S, Huang M, Feng X, Pei G, Hang Q, 
            Zhao Z, Gan B, Ma L, Chen J
  TITLE     USP7 substrates identified by proteomics analysis reveal the 
            specificity of USP7.
  JOURNAL   Genes Dev. 2022 Sep 1;36(17-18):1016-1030. doi: 
            10.1101/gad.349848.122. Epub 2022 Oct 27.
  PUBMED    36302555
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2022-09-1"; volume: "36(17-18):1016-1030. doi"; pages:
            " 10.1101/gad.349848.122. Epub 2022 Oct 27"
COMMENT     Sequence Label: pDonr 201_USP7_AA776-1102
FEATURES             Location/Qualifiers
     source          1..3245
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(49..148)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      201..287
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      379..406
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(541..1129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(621..640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1272..2081)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1995..2014
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2073..2092
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    2204..2303
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gatctcggaa atactccttt gcggtgggta attcactgtt atccatgaag cctgcttttt
       61 tgtacaaagt tggcattata aaaaagcatt gctcatcaat ttgttgcaac gaacaggtca
      121 ctatcagtca aaataaaatc attatttggg gcccgagatc catgctagcg ttaacgcgag
      181 agtagggaac tgccaggcat caaataaaac gaaaggctca gtcggaagac tgggcctttc
      241 gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg
      301 atttgaacgt tgtgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      361 ccaggcatca aactaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      421 ctcttcctgg ctagcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      481 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      541 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      601 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      661 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      721 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      781 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      841 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      901 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      961 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1021 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1081 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1141 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1201 catgagcttg cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta caaccaatta
     1261 accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     1321 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     1381 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     1441 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     1501 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
     1561 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     1621 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     1681 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     1741 gaatcaggat attcttctaa tacctggaat gctgtttttc cggggatcgc agtggtgagt
     1801 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     1861 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     1921 tgtttcagaa acaactctgg cgcatcgggc ttcccataca agcgatagat tgtcgcacct
     1981 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     2041 tttaatcgcg gcctcgacgt ttcccgttga atatggctca taacacccct tgtattactg
     2101 tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa
     2161 catcagagat tttgagacac gggccagagc tgcagctgga tggcaaataa tgattttatt
     2221 ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttc ttataatgcc
     2281 aactttgtac aagaaagctg ggttttagtt atggatttta atggcctttt caaggtaagt
     2341 gtagcgactc ctctttgggg ctttgttgaa gtggtcgagc cctagccaag gccgaggatg
     2401 agacatatta ccgggctgtg gctcaaagtc tttcaaattt acttcatact cgtcttcatt
     2461 tatgtactgg tgtcggccca tcattacaat tgcaaattta aacttctcaa actccttctc
     2521 ctggatgtcc agcaggctct ggattcgctt catcacttct cgaaaatgct cgccctggtg
     2581 tatcctcagc aaaaacggga ttccgaacgt tccgaagacc tctttgtgga aatgcgccac
     2641 tgtgacaagc atctcattct ctttgtctat gtccacctgg tccaaaggga tttcctctat
     2701 tcgaaacgtc cggctcgttg caggagataa acattctaat agttcatctt cttgatgaac
     2761 accaatgatt ttgtagctta caatttctag cagcctaagt ttccctgatg ctttctcccc
     2821 aagctccacg gcctttttac attcttctaa caggtcccgg acacacccat gcttgtctgg
     2881 atatagtgtt atttcctctt ccctaaattg gctgtttaac catatacatt taaaacttcg
     2941 cctgttctca aagtctgtga ttttcatctt aagctgctga tagtaaagtt tcttaggttg
     3001 tctaggcttg aagaactgta gaagatctct taaagtacct tcataattat gtctaagagg
     3061 attacctggg ccatccctat aaccttgaga cttgaaaaac tgcagcaaca ttggatctgt
     3121 gttgagcctc tgtgcaactg tctttgcaac ctgaaaataa ttcattctat ttgataacgt
     3181 aaccacaaat ccaggatcat tagggattgt tttatcacag aaaatgacat caacgcggtg
     3241 gtaga
//
