LOCUS       sequence-488953-        2888 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Entry vector for Gateway with USP9 (AA1-208).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Nie L, Wang C, Liu X, Teng H, Li S, Huang M, Feng X, Pei G, Hang Q, 
            Zhao Z, Gan B, Ma L, Chen J
  TITLE     USP7 substrates identified by proteomics analysis reveal the 
            specificity of USP7.
  JOURNAL   Genes Dev. 2022 Sep 1;36(17-18):1016-1030. doi: 
            10.1101/gad.349848.122. Epub 2022 Oct 27.
  PUBMED    36302555
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2022-09-1"; volume: "36(17-18):1016-1030. doi"; pages:
            " 10.1101/gad.349848.122. Epub 2022 Oct 27"
COMMENT     Sequence Label: pDonr 201_USP7_AA1-208
FEATURES             Location/Qualifiers
     source          1..2888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..48)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             40..849
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(107..126)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      992..1580
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1481..1500
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1715..1742)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1834..1920)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    1973..2072
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2706..2805)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
       61 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
      121 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
      181 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
      241 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
      301 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
      361 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
      421 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
      481 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
      541 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
      601 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
      661 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
      721 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
      781 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
      841 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
      901 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
      961 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1021 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1081 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1141 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1201 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1261 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1321 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1381 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1441 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1501 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1561 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1621 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1681 ttaccgctag ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt
     1741 ctgcttagtt tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg
     1801 ttgcttcaca acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac
     1861 cgacaaacaa cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg
     1921 atgcctggca gttccctact ctcgcgttaa cgctagcatg gatctcgggc cccaaataat
     1981 gattttattt tgactgatag tgacctgttc gttgcaacaa attgatgagc aatgcttttt
     2041 tataatgcca actttgtaca aaaaagcagg cttcatgaac caccagcagc agcagcagca
     2101 gcagaaagcg ggcgagcagc agttgagcga gcccgaggac atggagatgg aagcgggaga
     2161 tacagatgac ccaccaagaa ttactcagaa ccctgtgatc aatgggaatg tggccctgag
     2221 tgatggacac aacaccgcgg aggaggacat ggaggatgac accagttggc gctccgaggc
     2281 aacctttcag ttcactgtgg agcgcttcag cagactgagt gagtcggtcc ttagccctcc
     2341 gtgttttgtg cgaaatctgc catggaagat tatggtgatg ccacgctttt atccagacag
     2401 accacaccaa aaaagcgtag gattctttct ccagtgcaat gctgaatctg attccacgtc
     2461 atggtcttgc catgcacaag cagtgctgaa gataataaat tacagagatg atgaaaagtc
     2521 gttcagtcgt cgtattagtc atttgttctt ccataaagaa aatgattggg gattttccaa
     2581 ttttatggcc tggagtgaag tgaccgatcc tgagaaagga tttatagatg atgacaaagt
     2641 tacctttgaa gtctttgtac aggcggatgc tccccatgga gttgcgtggg attcaaagaa
     2701 gtaaaaccca gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg
     2761 ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgcagc
     2821 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2881 gaacaata
//
