LOCUS       sequence-488956-        5567 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Entry vector for Gateway with USP7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Nie L, Wang C, Liu X, Teng H, Li S, Huang M, Feng X, Pei G, Hang Q, 
            Zhao Z, Gan B, Ma L, Chen J
  TITLE     USP7 substrates identified by proteomics analysis reveal the 
            specificity of USP7.
  JOURNAL   Genes Dev. 2022 Sep 1;36(17-18):1016-1030. doi: 
            10.1101/gad.349848.122. Epub 2022 Oct 27.
  PUBMED    36302555
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2022-09-1"; volume: "36(17-18):1016-1030. doi"; pages:
            " 10.1101/gad.349848.122. Epub 2022 Oct 27"
COMMENT     Sequence Label: pDonr 201_USP7
FEATURES             Location/Qualifiers
     source          1..5567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(3388..3487)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3540..3626
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3718..3745
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(3880..4468)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3960..3979)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4611..5420)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5334..5353
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     5412..5431
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    join(5543..5567,1..75)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tagtgacctg ttcgttgcaa caaattgata agcaatgctt tcttataatg ccaactttgt
       61 acaagaaagc tgggtatcag ttatggattt taatggcctt ttcaaggtaa gtgtagcgac
      121 tcctctttgg ggctttgttg aagtggtcga gccctagcca aggccgagga tgagacatat
      181 taccgggctg tggctcaaag tctttcaaat ttacttcata ctcgtcttca tttatgtact
      241 ggtgtcggcc catcattaca attgcaaatt taaacttctc aaactccttc tcctggatgt
      301 ccagcaggct ctggattcgc ttcatcactt ctcgaaaatg ctcgccctgg tgtatcctca
      361 gcaaaaacgg gattccgaac gttccgaaga cctctttgtg gaaatgcgcc actgtgacaa
      421 gcatctcatt ctctttgtct atgtccacct ggtccaaagg gatttcctct attcgaaacg
      481 tccggctcgt tgcaggagat aaacattcta atagttcatc ttcttgatga acaccaatga
      541 ttttgtagct tacaatttct agcagcctaa gtttccctga tgctttctcc ccaagctcca
      601 cggccttttt acattcttct aacaggtccc ggacacaccc atgcttgtct ggatatagtg
      661 ttatttcctc ttccctaaat tggctgttta accatataca tttaaaactt cgcctgttct
      721 caaagtctgt gattttcatc ttaagctgct gatagtaaag tttcttaggt tgtctaggct
      781 tgaagaactg tagaagatct cttaaagtac cttcataatt atgtctaaga ggattacctg
      841 ggccatccct ataaccttga gacttgaaaa actgcagcaa cattggatct gtgttgagcc
      901 tctgtgcaac tgtctttgca acctgaaaat aattcattct atttgataac gtaaccacaa
      961 atccaggatc attagggatt gttttatcac agaaaatgac atcaacgcgg tggtagagat
     1021 ctcggaaata ctcctttgcg gtgggtaatt cactgttatc attttcaggg tcatccttct
     1081 gaaatactat gatgtcacca tccattagtt catcaagggc tttatcaaga gacacgtcat
     1141 agtcctgaat tctctctgtt aaattcggtt taacttcctc atagaggata aggctagtat
     1201 cttgaataaa tcctgctctg tcacacataa ctgggagcaa gtcacgtatt ttacaggata
     1261 ttggtgtgta gatatgccca cagtaattca agctccgcgt tttgggatca tacatcttca
     1321 aaaataacat tacatcatga tctttatcaa acttgggtaa ggtcgctcca ctagcagcca
     1381 gctcgggatc aactgtttcc aggaatattg tccaagggtt ttcattatca ctgagctcaa
     1441 tcattgtttt attgccgtcg gcttcattat ctaacattgc tggtcgtttt gttccattac
     1501 tccttgcttg catgggccac aatcgaattt gatcttgtgg aaatcccatg gtctgagaga
     1561 ggctctgaac aaactcagca agcgaggagt tcttcaatac tttgaacaca gtgtatttca
     1621 ctttttcttc atcgtacatg tcattccctt ggtggccaca aaactggtcc tctgcgacta
     1681 tctgcacttg catatagaga tgggcttcct gccgctcctt ccgcttctga gcctcgatcc
     1741 ttttctcttc ttgtaatcgc tccaccaact gctgaggaat atcatggtcg gtgaccgcct
     1801 gtaaaacttc actcagtttt gattccctga tgtagactaa catgtaagca ttagtgcagt
     1861 gtcgaacaga caggtcgtca tcgtgacccc cataattgtg ctcaattgct tcctctttag
     1921 tacaccttga caccacgtcg tcatcaaatt tacaccattt gccatcccct ttggggttta
     1981 gataaaccac ataatgtcca ccatgattat ctccactatg aaccaggact gcatgaagaa
     2041 tataatttgc agggtcctta ggatctgttt tttgcaaaaa ttcatcaagt ggtaactgct
     2101 ctgggaattc aaacctatca ttgatcttga tattttggtc cgtctgaggg tcatacataa
     2161 atctcatcag ttgtagatgt aacactggtg gcaatgttag gaatttcaca cctttctctg
     2221 cttcctgtaa gccatgttcc ccagcgtcgt atttattgtc cccatcgagc tgttctactg
     2281 ccacataatc cacaaatgat tcaaatatat ttttctttcc tttgatactt agctggatat
     2341 cataataatc ttctcttcta tcagaccgat agtctacttc tttacactgg atataggaca
     2401 ccattttgcc gcggaataat ttgggtatgg tgccctctac acaggtgcct ttcatcttat
     2461 tttccacatt atcgagcaac actcgacaaa gctcctgaac atcatgttgc atgaagctat
     2521 ctaaagtttc ccacccaaat gactttgtta acttttttgt tcctacaggt ttatcactat
     2581 gctgtaattc atagaacact ctttgtaatg ctaaagggac gcttttagac gaatcatccc
     2641 cctcggttgg catcatgtac acagcctttc gtagctgatt cgtgaaaaat aacgtctgta
     2701 gcaggctgtt catgtaacaa gtcgctccct gattctttaa gccgacgtag cctgtgtgct
     2761 tctttgaatc ccacgcaact ccatggggag catccgcctg tacaaagact tcaaaggtaa
     2821 ctttgtcatc atctataaat cctttctcag gatcggtcac ttcactccag gccataaaat
     2881 tggaaaatcc ccaatcattt tctttatgga agaacaaatg actaatacga cgactgaacg
     2941 acttttcatc atctctgtaa tttattatct tcagcactgc ttgtgcatgg caagaccatg
     3001 acgtggaatc agattcagca ttgcactgga gaaagaatcc tacgcttttt tggtgtggtc
     3061 tgtctggata aaagcgtggc atcaccataa tcttccatgg cagatttcgc acaaaacacg
     3121 gagggctaag gaccgactca ctcagtctgc tgaagcgctc cacagtgaac tgaaaggttg
     3181 cctcggagcg ccaactggtg tcatcctcca tgtcctcctc cgcggtgttg tgtccatcac
     3241 tcagggccac attcccattg atcacagggt tctgagtaat tcttggtggg tcatctgtat
     3301 ctcccgcttc catctccatg tcctcgggct cgctcaactg ctgctcgccc gctttctgct
     3361 gctgctgctg ctgctggtgg ttcatgaagc ctgctttttt gtacaaagtt ggcattataa
     3421 aaaagcattg ctcatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     3481 ttatttgggg cccgagatcc atgctagcgt taacgcgaga gtagggaact gccaggcatc
     3541 aaataaaacg aaaggctcag tcggaagact gggcctttcg ttttatctgt tgtttgtcgg
     3601 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gtgaagcaac
     3661 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa actaagcaga
     3721 aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttcctggc tagcggtaat
     3781 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3841 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3901 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3961 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4021 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4081 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4141 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4201 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4261 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4321 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4381 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4441 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4501 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagcttgc gccgtcccgt
     4561 caagtcagcg taatgctctg ccagtgttac aaccaattaa ccaattctga ttagaaaaac
     4621 tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt
     4681 tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca
     4741 agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc
     4801 ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt
     4861 gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc
     4921 tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg
     4981 agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg
     5041 cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat
     5101 acctggaatg ctgtttttcc ggggatcgca gtggtgagta accatgcatc atcaggagta
     5161 cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc
     5221 atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc
     5281 gcatcgggct tcccatacaa gcgatagatt gtcgcacctg attgcccgac attatcgcga
     5341 gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgacgtt
     5401 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     5461 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacg
     5521 ggccagagct gcagctggat ggcaaataat gattttattt tgactga
//
