LOCUS       sequence-488958-        2915 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Entry vector for Gateway with USP14 (AA886-1102).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Nie L, Wang C, Liu X, Teng H, Li S, Huang M, Feng X, Pei G, Hang Q, 
            Zhao Z, Gan B, Ma L, Chen J
  TITLE     USP7 substrates identified by proteomics analysis reveal the 
            specificity of USP7.
  JOURNAL   Genes Dev. 2022 Sep 1;36(17-18):1016-1030. doi: 
            10.1101/gad.349848.122. Epub 2022 Oct 27.
  PUBMED    36302555
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2022-09-1"; volume: "36(17-18):1016-1030. doi"; pages:
            " 10.1101/gad.349848.122. Epub 2022 Oct 27"
COMMENT     Sequence Label: pDonr 201_USP7_AA886-1102
FEATURES             Location/Qualifiers
     source          1..2915
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(169..268)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(380..399)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             391..1200
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(458..477)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1343..1931
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1832..1851
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2066..2093)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2185..2271)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    2324..2423
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 accagtacat aaatgaagac gagtatgaag taaatttgaa agactttgag ccacagcccg
       61 gtaatatgtc tcatcctcgg ccttggctag ggctcgacca cttcaacaaa gccccaaaga
      121 ggagtcgcta cacttacctt gaaaaggcca ttaaaatcca taactaaaac ccagctttct
      181 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
      241 ctatcagtca aaataaaatc attatttgcc atccagctgc agctctggcc cgtgtctcaa
      301 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct
      361 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcgagg
      421 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
      481 gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg
      541 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
      601 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
      661 gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cattccaggt attagaagaa
      721 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
      781 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg
      841 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
      901 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
      961 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     1021 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     1081 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     1141 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa
     1201 tcagaattgg ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc
     1261 gcaagctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1321 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1381 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1441 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1501 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1561 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1621 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1681 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1741 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1801 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1861 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1921 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1981 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcta
     2041 gccaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
     2101 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
     2161 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
     2221 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
     2281 agttccctac tctcgcgtta acgctagcat ggatctcggg ccccaaataa tgattttatt
     2341 ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc
     2401 aactttgtac aaaaaagcag gcttcatgac agactttgag aacaggcgaa gttttaaatg
     2461 tatatggtta aacagccaat ttagggaaga ggaaataaca ctatatccag acaagcatgg
     2521 gtgtgtccgg gacctgttag aagaatgtaa aaaggccgtg gagcttgggg agaaagcatc
     2581 agggaaactt aggctgctag aaattgtaag ctacaaaatc attggtgttc atcaagaaga
     2641 tgaactatta gaatgtttat ctcctgcaac gagccggacg tttcgaatag aggaaatccc
     2701 tttggaccag gtggacatag acaaagagaa tgagatgctt gtcacagtgg cgcatttcca
     2761 caaagaggtc ttcggaacgt tcggaatccc gtttttgctg aggatacacc agggcgagca
     2821 ttttcgagaa gtgatgaagc gaatccagag cctgctggac atccaggaga aggagtttga
     2881 gaagtttaaa tttgcaattg taatgatggg ccgac
//
