LOCUS       sequence-488959-        7793 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Entry vector for Gateway with PHIP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Nie L, Wang C, Liu X, Teng H, Li S, Huang M, Feng X, Pei G, Hang Q, 
            Zhao Z, Gan B, Ma L, Chen J
  TITLE     USP7 substrates identified by proteomics analysis reveal the 
            specificity of USP7.
  JOURNAL   Genes Dev. 2022 Sep 1;36(17-18):1016-1030. doi: 
            10.1101/gad.349848.122. Epub 2022 Oct 27.
  PUBMED    36302555
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2022-09-1"; volume: "36(17-18):1016-1030. doi"; pages:
            " 10.1101/gad.349848.122. Epub 2022 Oct 27"
COMMENT     Sequence Label: pDonr 201_PHIP
FEATURES             Location/Qualifiers
     source          1..7793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(117..216)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      269..355
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      447..474
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(609..1197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(689..708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1340..2149)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2063..2082
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2141..2160
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    2272..2371
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gatgaggaag tagagctccg atcgcagctc cgagaggcct ttcctctcac aagacataag
       61 gatccagtcg accatggtta agtatttcct tagagaattg gttctatctc cttcgaagcc
      121 tgcttttttg tacaaagttg gcattataaa aaagcattgc tcatcaattt gttgcaacga
      181 acaggtcact atcagtcaaa ataaaatcat tatttggggc ccgagatcca tgctagcgtt
      241 aacgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cggaagactg
      301 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
      361 gggagcggat ttgaacgttg tgaagcaacg gcccggaggg tggcgggcag gacgcccgcc
      421 ataaactgcc aggcatcaaa ctaagcagaa ggccatcctg acggatggcc tttttgcgtt
      481 tctacaaact cttcctggct agcggtaata cggttatcca cagaatcagg ggataacgca
      541 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      601 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      661 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      721 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      781 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      841 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      901 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      961 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1021 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1081 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1141 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1201 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1261 attttggtca tgagcttgcg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca
     1321 accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     1381 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     1441 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     1501 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     1561 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
     1621 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     1681 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
     1741 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1801 tttcacctga atcaggatat tcttctaata cctggaatgc tgtttttccg gggatcgcag
     1861 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1921 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1981 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaag cgatagattg
     2041 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     2101 tgttggaatt taatcgcggc ctcgacgttt cccgttgaat atggctcata acaccccttg
     2161 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     2221 caatgtaaca tcagagattt tgagacacgg gccagagctg cagctggatg gcaaataatg
     2281 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttctt
     2341 ataatgccaa ctttgtacaa gaaagctggg tctagatatc tcgagtgcca accaattaaa
     2401 ttagcttttg ctttttcagt caactttcgg actcgtcctc tactagaagt tccaaaagtt
     2461 aaagaggtgt cttcgaacaa cagctgcctt tgctcctctt cagagtcatc ctcattatag
     2521 aaagctgtcc ttcgaccttg atttctagtt ctcatgtggg gttcagagcc tttgagttct
     2581 tcaaactctt cttcctcatc tataggatca tctatctttt ttcggttact tctccttaac
     2641 actttgacac ttgcagggac taggagatct gcatctaatt tttgtgtctt catcttcctt
     2701 ttgggcttcc tacctccacg attctttttc tgtaacaaat cttcctttac attattagtt
     2761 tcagaaagaa aattgcatgt tgaagaagga agtacttcat ctctgatggg atgcacatta
     2821 ttttgctcta aatcttctgg ctttgcatac tgtagctttt tgggctttct accccttttc
     2881 ttgtgtataa tttcaccact attggtatta acttctactt taggtttcct tccaggtccc
     2941 ctcttcacaa gttttgatgg ctgtcctcca tggccattta cttgaatggt tcctggtaca
     3001 agagcgttgt tcttacaatc tccttgctca atgacagctg atgactttga aagagtggac
     3061 gcctttggga gtacagatga cttcatttta cgtttgactg gcttttcctt ttccatgttt
     3121 tcttttgcag aattattcct agtgccatga ctaaaactgg attgaccagg actggaaaga
     3181 gtattcaaag ctttggaatg tttcacagaa ttctctagta ttgttttccc tggtattgca
     3241 gatgcattag cttttgtaat aaaagtcttt gcagctgaag aagtagatgg ttgctcagtg
     3301 acaactggat ctacaaccac tcggttgctt ctggttcgaa ccacagaact agattctgtt
     3361 ttaccgttta tctgagcagc attgtgtctt ggcggtattg atcgtgtagg tgtagagaat
     3421 gcagaggtag agctttctga ttttagctgg ggttttaaga tccttttttt cctttcaggg
     3481 cttgatgcag cactactgga aacagagctg cttctgtttc ttttcttcct ccttttggtt
     3541 atggtatttc ttttatgaaa acgaagagca gatttataat ctgataaaac tgaactaatg
     3601 tgttcttcaa agaaagcaga caggcgcaaa ctcatgctgt aaatccttga tcttttgctt
     3661 ggtgtatatg ctttggaatt actgaaaata agtctgacat ctttacataa ctccattggt
     3721 gactcataat tcccagcctc taaagtttct ctaacggtag caaaatccat tggagtgtca
     3781 atgatgtctc tgtagtctgg atattcaagg agatctaccg gctgacggaa aggctctgaa
     3841 tcttcacatt gaaatatgag atttaacaat tcttcacact gtttcttcca tgcttgaata
     3901 tcgtaagact gggctctatt acgtaatctt cttctaggct gatggtcctt cctttttcga
     3961 gtagaagttc ctggcacatc agcatctttc tcttcatcct cagaatcaga caaaactttc
     4021 ttcttcattg aattataaag tggaattatg ttataacaag tctgatcctt tataaaatgt
     4081 agaagaagat cagtcacgaa tttagcagat ttcacaatag ggcttccagg ctcattaaat
     4141 gttcgtgtat tatgctctat atatcgaact tcccacatta gggaagaaac ccgcctgtaa
     4201 aacctgtttt ccagtctttg tttaattgta cttagatccg ttggatatgc cactactgtg
     4261 caatacatgg gataggcttg cagatccacg ggggccacaa atgctgaggc aatatctagt
     4321 gtcatcaact ggtttattcc tgccacaatt ctttcacatt cttcatccct gggattggta
     4381 ccccattctc catcaagagg tttatagatt agtgatctgc actcaccatc agttaaagga
     4441 acactggtac ctagttcttc aggaaataca gcattattag gtataagctc catatcccaa
     4501 ggactcatct tttctgtatc tccattgtcc cagcaaacat tgtagcattg aaacagacta
     4561 tcagggtact caagttgaag aggttcctgg ctttcgattg ttccaaacca ccaggcatca
     4621 tctatgacag acctgaagcg gtcacctata ttccatcgcc tgtattttgc atcatcaaat
     4681 tgttgtctca agactaggaa atctataacg tcaggcatat catggtattt catggtaaat
     4741 gatccgccag tcagtttacc agtatcagga tctagaaaag caagtttaag gcagcaaagg
     4801 gtaggtaatc ccacttcata ctttatgcca actattttca taagttcttg ttcccgtagc
     4861 tccattttat gccatggttg ttttttggga ttgatactat atattttatt tttccgggcc
     4921 atttcgacat aggcttcatg tccttgtcgg aaataataaa cctcatcacc catctgtggc
     4981 acaaatggac atcttcgggg aatggtatct gtaatccatg ttgatggcaa ccattcttct
     5041 aatgtcaaac cattttcagt tagttctccc acagccaatc tcttttgttt tctttctttg
     5101 ggcttctttt tctttggtga tattggtcca tctttttctt catttacttt tttcttttcc
     5161 tttttaatct gtttttgctt ctgtttttca gattcttctt cttcatctga actgctttct
     5221 gctttcttga ttttattctt aggaactttc tttggtggct gcagattaat tcctgcatct
     5281 gctgtccagt cagagtaatc actggagtag tcactagaac tgccatcact gtgccatgct
     5341 ctctcttctt cttcggatgt tccaccactg acagcaacta cttcgccttc atctgaagaa
     5401 ctactgccat tttctatctc ttctgagggt ctaggagtct cttccaatgc agatcttgta
     5461 cgataattgt gttgatttgt ctgttgcttt ttggattctc caagatccag gaaatgctca
     5521 tgagcatgat tctttgagac agtgggtatt ttattctctt ttggaacagt taagtgtttt
     5581 cttttctctt ctgacctgta agtctttatt tcttcttctc cctttgcagt tctccattct
     5641 tcttgcctac tggctacacc agctgatagc tcgggtacta ccacccttcg actccaagct
     5701 accagatccc gctctgtggc tatttcactt cttggtgcgt tgctgtgcat ttgccgtaca
     5761 ccttcaattt gtccactacg tcttagtcct acgtttggtg gtgaatgaac ctctgaggta
     5821 gaacttatgg agcctctact taaacggctg gtattactga tacctgcttc accagaacgt
     5881 ctcaggtctt gctcctgttg tagtctttga atcatgctgt ccagtgggct gatctcctgg
     5941 tttgcttgct gacttaaaac ttgattcagt cctgaggaag ttactcccat ctgagggatg
     6001 agttgctcct ccctgcaatt ttcacggcca ggaactaatc tttgatatct tgatggatga
     6061 gggttaccat caacatcaac caaaaaaggg ggaggcataa gatgaggtgc ttgctgagtc
     6121 tgttcatcta atacaaaatt gttggcatca cgaataagtg gccgataatc actatgaaag
     6181 aacatctgat ctgctatctt gtcatatttg ctactggacc caaagccaaa aattaaaaga
     6241 tgtccatgag agtctgtgca tgcaaaatgc tgaccatcag gagagcattt gcagtcaaat
     6301 actgcgccat gtccttggcc ttcaatcata ttgaaataag atcgtatttt gactcctctt
     6361 gccagatccc acactatcac gtttccatca tgaccagcag aaaagagaac tctaggatcg
     6421 aacgggtgtg gttcaagaac aaatacctca tcttcatgac ccatcaggac atgaattagt
     6481 tgaccagtgt aagaattcca aactttcaga gtcatgttat taactgcagt tataactgta
     6541 ttgtcatgtc gatcccaagc taccatagta accttcattt ttgtgatttt atcttctatt
     6601 ccttgaaggt tttggcctgc tggacgagta gccatatcca acaaaatgct cttccactct
     6661 cttcgtttaa attgccaaat acgtgctgtc ccatcacgac tgccacttac aaacctgtta
     6721 ctagtgttgg aaaactggat actgtcaact ttgtcagtat gaaactccaa ttctgatatt
     6781 ttctctggct gacctgatcc aaaaaaataa acccgaataa tatgatctgt gcttcccgtc
     6841 gccagaaaca ttccaccagc actaaaagaa gaacagatca tttgaactcc aggccgaggg
     6901 cgctctgtaa attttgcagg tcttgggttt attttaaggg ttccagcatc ccagagccaa
     6961 aaacaaatag tgccatctgc cccagtagaa gatagatatc tctttgagcc actgcacaat
     7021 ggtgagaact gtagtgatgt aatagatgca ctatggccct gaagaacagc caaaggtgca
     7081 caggttcgaa gacaccagac tcggatcatt ttatcacaac ttccagctgc tatcatggta
     7141 ttctcatagt ttacagccat gtctgatatt tcagcagcat gtcctcttaa ggtagctaac
     7201 aacctcccat catctgttgc ccatattttc acaagacagt catcagaacc agtaaatatc
     7261 cgtctgccag ttcgatcaaa agttacacag tacacagatg acaagtgtcc aagaattcgt
     7321 ttatgcattt tcatgtgctg atacactgca gttggaacaa gtcgctcaag tctgtatttc
     7381 ccattcagct tccttgaaaa cagagtatcc gcaatgctgg gtgggctacc atagttaact
     7441 ggtgactcag gtggtcttcc acagtgcaac gcagccagag cagatccttt ccacacaaca
     7501 tgcttgcagc ttttatttgt gcgtagtaaa gactgtcttc cagctcctaa taaagtttgt
     7561 actccaggaa cactttgagg aatttcttgt tcaagaagag gtcctagtcg atgacatatt
     7621 tgcagcaagt gatcaggtgc taagtgtctg taatacttca ccagattctg gtaggtcctg
     7681 ggatgctcct tcccggtcca gtcggtgcgc cggggcagca gctccttctc ggccacctcg
     7741 cggatcagca cctgagccgc ctgctgacag ggtccatctt ccaggaaccg ggc
//
