LOCUS       sequence-488960-        3968 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Entry vector for Gateway with USP12 (AA535-1102).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Nie L, Wang C, Liu X, Teng H, Li S, Huang M, Feng X, Pei G, Hang Q, 
            Zhao Z, Gan B, Ma L, Chen J
  TITLE     USP7 substrates identified by proteomics analysis reveal the 
            specificity of USP7.
  JOURNAL   Genes Dev. 2022 Sep 1;36(17-18):1016-1030. doi: 
            10.1101/gad.349848.122. Epub 2022 Oct 27.
  PUBMED    36302555
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2022-09-1"; volume: "36(17-18):1016-1030. doi"; pages:
            " 10.1101/gad.349848.122. Epub 2022 Oct 27"
COMMENT     Sequence Label: pDonr 201_USP7_AA535-1102
FEATURES             Location/Qualifiers
     source          1..3968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1790..1889)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      1942..2028
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2120..2147
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(2282..2870)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2362..2381)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3013..3822)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3736..3755
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3814..3833
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    join(3945..3968,1..76)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atagtgacct gttcgttgca acaaattgat aagcaatgct ttcttataat gccaactttg
       61 tacaagaaag ctgggtttta gttatggatt ttaatggcct tttcaaggta agtgtagcga
      121 ctcctctttg gggctttgtt gaagtggtcg agccctagcc aaggccgagg atgagacata
      181 ttaccgggct gtggctcaaa gtctttcaaa tttacttcat actcgtcttc atttatgtac
      241 tggtgtcggc ccatcattac aattgcaaat ttaaacttct caaactcctt ctcctggatg
      301 tccagcaggc tctggattcg cttcatcact tctcgaaaat gctcgccctg gtgtatcctc
      361 agcaaaaacg ggattccgaa cgttccgaag acctctttgt ggaaatgcgc cactgtgaca
      421 agcatctcat tctctttgtc tatgtccacc tggtccaaag ggatttcctc tattcgaaac
      481 gtccggctcg ttgcaggaga taaacattct aatagttcat cttcttgatg aacaccaatg
      541 attttgtagc ttacaatttc tagcagccta agtttccctg atgctttctc cccaagctcc
      601 acggcctttt tacattcttc taacaggtcc cggacacacc catgcttgtc tggatatagt
      661 gttatttcct cttccctaaa ttggctgttt aaccatatac atttaaaact tcgcctgttc
      721 tcaaagtctg tgattttcat cttaagctgc tgatagtaaa gtttcttagg ttgtctaggc
      781 ttgaagaact gtagaagatc tcttaaagta ccttcataat tatgtctaag aggattacct
      841 gggccatccc tataaccttg agacttgaaa aactgcagca acattggatc tgtgttgagc
      901 ctctgtgcaa ctgtctttgc aacctgaaaa taattcattc tatttgataa cgtaaccaca
      961 aatccaggat cattagggat tgttttatca cagaaaatga catcaacgcg gtggtagaga
     1021 tctcggaaat actcctttgc ggtgggtaat tcactgttat cattttcagg gtcatccttc
     1081 tgaaatacta tgatgtcacc atccattagt tcatcaaggg ctttatcaag agacacgtca
     1141 tagtcctgaa ttctctctgt taaattcggt ttaacttcct catagaggat aaggctagta
     1201 tcttgaataa atcctgctct gtcacacata actgggagca agtcacgtat tttacaggat
     1261 attggtgtgt agatatgccc acagtaattc aagctccgcg ttttgggatc atacatcttc
     1321 aaaaataaca ttacatcatg atctttatca aacttgggta aggtcgctcc actagcagcc
     1381 agctcgggat caactgtttc caggaatatt gtccaagggt tttcattatc actgagctca
     1441 atcattgttt tattgccgtc ggcttcatta tctaacattg ctggtcgttt tgttccatta
     1501 ctccttgctt gcatgggcca caatcgaatt tgatcttgtg gaaatcccat ggtctgagag
     1561 aggctctgaa caaactcagc aagcgaggag ttcttcaata ctttgaacac agtgtatttc
     1621 actttttctt catcgtacat gtcattccct tggtggccac aaaactggtc ctctgcgact
     1681 atctgcactt gcatatagag atgggcttcc tgccgctcct tccgcttctg agcctcgatc
     1741 cttttctctt cttgtaatcg ctccaccaac tgctgaggaa tatccatgaa gcctgctttt
     1801 ttgtacaaag ttggcattat aaaaaagcat tgctcatcaa tttgttgcaa cgaacaggtc
     1861 actatcagtc aaaataaaat cattatttgg ggcccgagat ccatgctagc gttaacgcga
     1921 gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcggaaga ctgggccttt
     1981 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg
     2041 gatttgaacg ttgtgaagca acggcccgga gggtggcggg caggacgccc gccataaact
     2101 gccaggcatc aaactaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa
     2161 actcttcctg gctagcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     2221 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     2281 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     2341 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     2401 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     2461 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2521 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2581 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2641 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2701 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2761 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2821 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2881 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2941 tcatgagctt gcgccgtccc gtcaagtcag cgtaatgctc tgccagtgtt acaaccaatt
     3001 aaccaattct gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
     3061 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
     3121 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
     3181 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
     3241 atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg
     3301 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
     3361 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca
     3421 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
     3481 tgaatcagga tattcttcta atacctggaa tgctgttttt ccggggatcg cagtggtgag
     3541 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     3601 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     3661 atgtttcaga aacaactctg gcgcatcggg cttcccatac aagcgataga ttgtcgcacc
     3721 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     3781 atttaatcgc ggcctcgacg tttcccgttg aatatggctc ataacacccc ttgtattact
     3841 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
     3901 acatcagaga ttttgagaca cgggccagag ctgcagctgg atggcaaata atgattttat
     3961 tttgactg
//
