LOCUS       sequence-488961-        2990 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Entry vector for Gateway with USP10 (AA535-776).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Nie L, Wang C, Liu X, Teng H, Li S, Huang M, Feng X, Pei G, Hang Q, 
            Zhao Z, Gan B, Ma L, Chen J
  TITLE     USP7 substrates identified by proteomics analysis reveal the 
            specificity of USP7.
  JOURNAL   Genes Dev. 2022 Sep 1;36(17-18):1016-1030. doi: 
            10.1101/gad.349848.122. Epub 2022 Oct 27.
  PUBMED    36302555
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2022-09-1"; volume: "36(17-18):1016-1030. doi"; pages:
            " 10.1101/gad.349848.122. Epub 2022 Oct 27"
COMMENT     Sequence Label: pDonr 201_USP7_AA535-776
FEATURES             Location/Qualifiers
     source          1..2990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(545..1354)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1268..1287
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1346..1365
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1477..1576
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2312..2411)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2464..2550
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2642..2669
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     rep_origin      complement(join(2804..2990,1..402))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2884..2903)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
       61 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      121 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      181 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      241 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      301 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      361 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      421 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgagc
      481 ttgcgccgtc ccgtcaagtc agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt
      541 ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
      601 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
      661 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
      721 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
      781 cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag
      841 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
      901 attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
      961 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     1021 gatattcttc taatacctgg aatgctgttt ttccggggat cgcagtggtg agtaaccatg
     1081 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     1141 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     1201 gaaacaactc tggcgcatcg ggcttcccat acaagcgata gattgtcgca cctgattgcc
     1261 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     1321 gcggcctcga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     1381 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     1441 gattttgaga cacgggccag agctgcagct ggatggcaaa taatgatttt attttgactg
     1501 atagtgacct gttcgttgca acaaattgat aagcaatgct ttcttataat gccaactttg
     1561 tacaagaaag ctgggtttta atcattttca gggtcatcct tctgaaatac tatgatgtca
     1621 ccatccatta gttcatcaag ggctttatca agagacacgt catagtcctg aattctctct
     1681 gttaaattcg gtttaacttc ctcatagagg ataaggctag tatcttgaat aaatcctgct
     1741 ctgtcacaca taactgggag caagtcacgt attttacagg atattggtgt gtagatatgc
     1801 ccacagtaat tcaagctccg cgttttggga tcatacatct tcaaaaataa cattacatca
     1861 tgatctttat caaacttggg taaggtcgct ccactagcag ccagctcggg atcaactgtt
     1921 tccaggaata ttgtccaagg gttttcatta tcactgagct caatcattgt tttattgccg
     1981 tcggcttcat tatctaacat tgctggtcgt tttgttccat tactccttgc ttgcatgggc
     2041 cacaatcgaa tttgatcttg tggaaatccc atggtctgag agaggctctg aacaaactca
     2101 gcaagcgagg agttcttcaa tactttgaac acagtgtatt tcactttttc ttcatcgtac
     2161 atgtcattcc cttggtggcc acaaaactgg tcctctgcga ctatctgcac ttgcatatag
     2221 agatgggctt cctgccgctc cttccgcttc tgagcctcga tccttttctc ttcttgtaat
     2281 cgctccacca actgctgagg aatatccatg aagcctgctt ttttgtacaa agttggcatt
     2341 ataaaaaagc attgctcatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     2401 atcattattt ggggcccgag atccatgcta gcgttaacgc gagagtaggg aactgccagg
     2461 catcaaataa aacgaaaggc tcagtcggaa gactgggcct ttcgttttat ctgttgtttg
     2521 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgtgaag
     2581 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaactaag
     2641 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactcttcc tggctagcgg
     2701 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2761 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2821 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2881 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2941 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
//
