LOCUS       sequence-488962-        3329 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Entry vector for Gateway with USP11 (AA535-889).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Nie L, Wang C, Liu X, Teng H, Li S, Huang M, Feng X, Pei G, Hang Q, 
            Zhao Z, Gan B, Ma L, Chen J
  TITLE     USP7 substrates identified by proteomics analysis reveal the 
            specificity of USP7.
  JOURNAL   Genes Dev. 2022 Sep 1;36(17-18):1016-1030. doi: 
            10.1101/gad.349848.122. Epub 2022 Oct 27.
  PUBMED    36302555
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2022-09-1"; volume: "36(17-18):1016-1030. doi"; pages:
            " 10.1101/gad.349848.122. Epub 2022 Oct 27"
COMMENT     Sequence Label: pDonr 201_USP7_AA535-889
FEATURES             Location/Qualifiers
     source          1..3329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      830..1418
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1319..1338
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1553..1580)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1672..1758)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    1811..1910
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2985..3084)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3196..3215)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(3207..3329,1..687)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3274..3293)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
       61 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
      121 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
      181 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
      241 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
      301 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
      361 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
      421 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
      481 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
      541 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
      601 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
      661 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
      721 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
      781 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
      841 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      901 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      961 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     1021 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     1081 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     1141 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     1201 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     1261 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     1321 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     1381 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     1441 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     1501 attctgtgga taaccgtatt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     1561 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     1621 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     1681 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     1741 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     1801 tctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat
     1861 tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagcaggct tcatggatat
     1921 tcctcagcag ttggtggagc gattacaaga agagaaaagg atcgaggctc agaagcggaa
     1981 ggagcggcag gaagcccatc tctatatgca agtgcagata gtcgcagagg accagttttg
     2041 tggccaccaa gggaatgaca tgtacgatga agaaaaagtg aaatacactg tgttcaaagt
     2101 attgaagaac tcctcgcttg ctgagtttgt tcagagcctc tctcagacca tgggatttcc
     2161 acaagatcaa attcgattgt ggcccatgca agcaaggagt aatggaacaa aacgaccagc
     2221 aatgttagat aatgaagccg acggcaataa aacaatgatt gagctcagtg ataatgaaaa
     2281 cccttggaca atattcctgg aaacagttga tcccgagctg gctgctagtg gagcgacctt
     2341 acccaagttt gataaagatc atgatgtaat gttatttttg aagatgtatg atcccaaaac
     2401 gcggagcttg aattactgtg ggcatatcta cacaccaata tcctgtaaaa tacgtgactt
     2461 gctcccagtt atgtgtgaca gagcaggatt tattcaagat actagcctta tcctctatga
     2521 ggaagttaaa ccgaatttaa cagagagaat tcaggactat gacgtgtctc ttgataaagc
     2581 ccttgatgaa ctaatggatg gtgacatcat agtatttcag aaggatgacc ctgaaaatga
     2641 taacagtgaa ttacccaccg caaaggagta tttccgagat ctctaccacc gcgttgatgt
     2701 cattttctgt gataaaacaa tccctaatga tcctggattt gtggttacgt tatcaaatag
     2761 aatgaattat tttcaggttg caaagacagt tgcacagagg ctcaacacag atccaatgtt
     2821 gctgcagttt ttcaagtctc aaggttatag ggatggccca ggtaatcctc ttagacataa
     2881 ttatgaaggt actttaagag atcttctaca gttcttcaag cctagacaac ctaagaaact
     2941 ttactatcag cagcttaaga tgaaaatcac agactttgag taaaacccag ctttcttgta
     3001 caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
     3061 cagtcaaaat aaaatcatta tttgccatcc agctgcagct ctggcccgtg tctcaaaatc
     3121 tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt
     3181 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc
     3241 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
     3301 ggcaatcagg tgcgacaatc tatcgcttg
//
