LOCUS       sequence-488992-        3427 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Gateway Entry vector for human G1B3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3427)
  AUTHORS   Merienne N, Vachey G, de Longprez L, Meunier C, Zimmer V, Perriard 
            G, Canales M, Mathias A, Herrgott L, Beltraminelli T, Maulet A, 
            Dequesne T, Pythoud C, Rey M, Pellerin L, Brouillet E, Perrier AL, 
            du Pasquier R, Deglon N
  TITLE     The Self-Inactivating KamiCas9 System for the Editing of CNS Disease
            Genes.
  JOURNAL   Cell Rep. 2017 Sep 19;20(12):2980-2991. doi: 
            10.1016/j.celrep.2017.08.075.
  PUBMED    28930690
REFERENCE   2  (bases 1 to 3427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.08"; journalName: "Cell Rep"; date: 
            "2017-09-19- 19"; volume: "20"; issue: "12"; pages: "2980-2991"
COMMENT     Sequence Label: pENTR4-G1B3
FEATURES             Location/Qualifiers
     source          1..3427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1656..1755)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1867..1886)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1878..2687
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPGDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1945..1964)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2777..3365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3266..3285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggctcca ccatgggaac caattcagtc
      481 gactggatcc ggtaccgaat tcgagctcgg taccagatct aacatatcct ggtgtggagt
      541 aggggacgct gctctgacag aggctcgggg gcctgagctg gctctgtgag ctggggagga
      601 ggcagacagc caggccttgt ctgcaagcag acctggcagc attgggctgg ccgcccccca
      661 gggcctcctc ttcatgccca gtgaatgact caccttggca cagacacaat gttcggggtg
      721 ggcacagtgc ctgcttcccg ccgcacccca gcccccctca aatgccttcc gagaagccca
      781 ttgagcaggg ggcttgcatt gcaccccagc ctgacagcct ggcatcttgg gataaaagca
      841 gcacagcccc ctaggggctg cccttgctgt gtggcgccac cggcggtgga gaacaaggct
      901 ctattcagcc tgtgcccagg aaaggggatc aggggatgcc caggcatgga cagtgggtgg
      961 caggggggga gaggagggct gtctgcttcc cagaagtcca aggacacaaa tgggtgaggg
     1021 gagagctctc cccatagctg ggctgcggcc caaccccacc ccctcaggct atgccagggg
     1081 gtgttgccag gggcacccga tcttcctctt catgcccagt gaatgactca ccttggcaca
     1141 gacacaatgt tcggggtggg cacagtgcct gcttcccgcc gcaccccagc ccccctcaaa
     1201 tgccttccga gaagcccatt gagcaggccc gatcttcctc ttcatgccca gtgaatgact
     1261 caccttggca cagacacaat gttcggggtg ggcacagtgc ctgcttcccg ccgcacccca
     1321 gcccccctca aatgccttcc gagaagccca ttgagcaggc ccgatcttcc tcttcatgcc
     1381 cagtgaatga ctcaccttgg cacagacaca atgttcgggg tgggcacagt gcctgcttcc
     1441 cgccgcaccc cagcccccct caaatgcctt ccgagaagcc cattgagcag gcccgggcat
     1501 cgccagtcta gcccactcct tcataaagcc ctcgcatccc aggagcgagc agagccagag
     1561 caggttggag aggagacgca tcacctccgc tgctcgcggg gatcctctag agtcgacgga
     1621 tccggggaat tcgcggccgc actcgagata tctagaccca gctttcttgt acaaagttgg
     1681 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     1741 taaaatcatt atttgccatc cagctgcagc tctggcccgt gtctcaaaat ctctgatgtt
     1801 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     1861 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     1921 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     1981 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     2041 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     2101 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctggtgat gcatggttac
     2161 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     2221 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     2281 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     2341 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     2401 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     2461 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     2521 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     2581 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     2641 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     2701 attggttgta acattattca gattgggccc cgttccactg agcgtcagac cccgtagaaa
     2761 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     2821 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     2881 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     2941 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3001 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3061 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3121 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3181 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3241 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3301 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3361 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3421 acatgtt
//
