LOCUS       sequence-488997-       10024 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Express NanoLuc-TCF4(biased)-beta-Catenin-HaloTag Fusion Protein in 
            Mammalian Cells under a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10024)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NanoLuc-TCF4(biased)-beta-Catenin-HaloTag
            Bidi...
FEATURES             Location/Qualifiers
     source          1..10024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(747..1541)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(858..877)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1468..1487
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1573..1620)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    1630..1663
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    1778..1899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1869..1888
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(1986..2870)
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             complement(2886..2906)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             complement(2928..5270)
                     /codon_start=1
                     /gene="CTNNB1"
                     /product="protein involved both in cell-cell adhesion as 
                     part of the cadherin protein complex, and in gene 
                     transcription as part of the Wnt signaling pathway"
                     /label=CTNNB1
                     /note="also known as catenin beta-1; related to Drosophila 
                     armadillo"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     CDS             complement(2928..5270)
                     /codon_start=1
                     /gene="Homo sapiens CTNNB1"
                     /product="human cadherin-associated protein that also plays
                     a role in the canonical Wnt signaling pathway"
                     /label=beta-catenin
                     /note="also known as catenin beta-1"
                     /translation="MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPS
                     LSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLD
                     EGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDE
                     DQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLS
                     HHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQK
                     MVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTT
                     SRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGME
                     GLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRE
                     DITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNL
                     ALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGA
                     LHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEG
                     ATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETA
                     DLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGL
                     PDLGHAQDLMDGLPPGDSNQLAWFDTDL"
     primer_bind     complement(5283..5302)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5284..5302)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5344..5363)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          5346..5478
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(5614..5817)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5643..5663)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5818..6121
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6241..6259
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6241..6258
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             6288..6800
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     polyA_signal    8881..8962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        9041..9398
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9041..9232
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9064..9084)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9249..9384
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      9423..9432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9429..9827
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    9851..9899
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaatcag aagaactcgt caagaaggcg atagaaggcg
      781 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
      841 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
      901 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
      961 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg
     1021 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     1081 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     1141 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     1201 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     1261 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     1321 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg
     1381 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     1441 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     1501 gcggccggag aacctgcgtg caatccatct tgttcaatca tgatctgcct cctgggttta
     1561 gttcctcacc ttgtcgtatt atactatgcc gatatactat gccgatgatt aattgtcaac
     1621 agtggatcca taacttcgta taatgtatgc tatacgaagt tatattagaa ttcttaatta
     1681 agattttacc acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct
     1741 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     1801 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1861 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc
     1921 ttgctcagcg gtggcagcag ccaaatcagc ttgcatgcct gcaggtcgac tctagaattc
     1981 tattaaccgg aaatctccag agtagacagc cagcgcgcga tctcgctgcc gatcaggtcc
     2041 gggttgtctt cttgcagcag attcagaccc gggccgatgt ccacagcctt gcagttaggc
     2101 aggcttttgg ccaggcgagc ggcttcggcc ggtgggatca gaacgcctgg ggtgccccag
     2161 aacagcagct tcgggacagg ggactggtgc agccagtcca tgtattcttc gaccagcgcg
     2221 acgatgttcg ctggctcacc ggcgattggc agctcgtttg ggaagcgcca cagtggctcg
     2281 cggtcaacag gattcaggaa cggctcgcgg taatggtcca tctcgacttc agtcagcggg
     2341 cggacgacac ccatcggcag cgtaccctcg ataaaaacgt tctgatcgat gatcagcttg
     2401 cggccgacgt cggtggtgcg gaaggcctgg aaggtctcgc gggcaaattc tggccattcg
     2461 tcccaggtcg ggatagggcg gatgaactcc ataaatgcaa tacctttgac gcgctctgga
     2521 ttgcgcttgg cccagtggaa acccagagcg gagccccagt cgtgaatgac caggacgacc
     2581 tcttccagac ccagggcttc gatgaaggca tccatgaagc ggacgtggtc gtcgaagaaa
     2641 taacccaggt ctggtttgtc ggatttgccc ataccgatca ggtctggagc aatgcagcga
     2701 tgggtcggtg caacatgcgg gatgatgttg cgccacacgt aggaggaggt cgggttaccg
     2761 tgcaggaaca gcacaggggt gccatcgcgc ggaccaacat cgacgtagtg catgcgctcg
     2821 cccaggactt ccacataatg ggggtcgaat ggaaagccag taccgatttc ggatccatcg
     2881 ttatcgctct gaaagtacag atcctcagtg gttggctcga gagaaaccag gtcagtatca
     2941 aaccaggcca gctgattgct gtcacctgga ggcagcccat ccatgaggtc ctgggcatgc
     3001 cccagatctg gcagcccatc aactggatag tcagcaccag ggtggtggcc acccatctca
     3061 tgttccatca tggggtccat acccaaggca tcctggccat atccaccaga gtgaaaagaa
     3121 cgatagctag gatcatcctg gcgatatcca aggggttctc cctgggcacc aatatcaagt
     3181 ccaagatcag cagtctcatt ccaagccatt ggctctgttc tgaagagaga gctggtcagc
     3241 tcaactgaaa gccgtttctt gtaatcttgt ggcttgtcct cagacattcg gaacaaaaca
     3301 gcagctgcat atgtcgccac accttcattc ctagagtgaa gtaactctgt cagaggagct
     3361 gtggctccct cagcttcaat agcttctgca gcttccttgt cctgagcaag ttcacagagg
     3421 acccctgcag ctactctttg gatgttttca atgggagaat aaagcagctg cacaaacaat
     3481 ggaatggtat ttagtcctct gataacaatt cggttgtgaa catcccgagc taggatgtga
     3541 agggctccgg tacaaccttc aactatttct tccatgcgga ccccctccac aaattgctgc
     3601 tgtgtcccac ccatggacgt acggcgctgg gtatcctgat gtgcacgaac aagcaactga
     3661 actagtcgtg gaatggcacc ctgctcacgc aaaggtgcat gatttgcggg acaaagggca
     3721 agatttcgaa tcaatccaac agtagccttt atcagaggcc agtgggatgg tgggtgtaag
     3781 agcttaacca caactggtag tccatagtga aggcgaactg cattctgggc catctctgct
     3841 tcttggtgtc ggctggtcag atgacgaaga gcacagatgg caggctcagt gatgtcttcc
     3901 ctgtcaccag cccgaaggac agtacgcaca agagcctcta taccacccac ttggcagacc
     3961 atcatcttgt tcttataatt attgcaagtg aggttagaaa gaattccagc tgcacaggtg
     4021 accacattta tatcatctga acccagaagc tgaacaagag tcccaaggag accttccatc
     4081 ccttcctgtt tagttgcagc atctgaaaga ttcctgagag tccaaagaca gttctgaaca
     4141 agacgttgac ttggatctgt caggtgaagt cctaaagctt gcattccacc agcttctaca
     4201 atagccggct tattactaga gcagacagat agcaccttca gcactctgct tgtggtccac
     4261 agtagttttt cgtaagtata ggtcctcatt atatttacta aagcttgggg tccaccacta
     4321 gccagtatga tgagcttgct ttcttggttg ccataagcta aaatttgaag gcagtctgtc
     4381 gtaatagcca agaatttaac atttgttttg ttgagcaagg caaccatttt ctgcagccca
     4441 ccagctaaac gcactgccat tttagctcct tcttgatgta ataaaaggtt gtggagagtt
     4501 gtaatggcat aaaacaacac agaatccact ggtgaaccaa gcattttcac cagggcagga
     4561 atgcctccag acttaaagat ggccagtaag ccctcacgat gatgggaaag gttatgcaag
     4621 gtcccagcgg tacaacgagc tgtttctaca tcatttgtat tctgcatggt acgtacaata
     4681 gcagacacca tctgaggaga acgcatgata gcgtgtctgg aagcttcctt tttagaaagc
     4741 tgatggacca taactgcagc cttattaacc accacctggt cctcgtcatt tagcagtttt
     4801 gtcagttcag ggattgcacg tgtggcaagt tctgcatcat cttgatagtt aatcaagttt
     4861 acaactgcat gtttcagcat ctgtgatggt tcagccaaac gctggacatt agtgggatga
     4921 gcagcatcaa actgtgtaga tgggatctgc atgccctcat ctaatgtctc agggaacata
     4981 gcagctcgta ccctctgagc tcgagtcatt gcatactgtc catcaatatc agctacttgt
     5041 tcttgagtga aggactgaga aaatccctgt tcccactcat acaggacttg ggaggtatcc
     5101 acatcctctt cctcaggatt gcctttacca ctcagagaag gagctgtggt agtggcacca
     5161 gaatggattc cagagtccag gtaagactgt tgctgccagt gactaacagc cgcttttctg
     5221 tctggttcca tggccatgtc caactccatc aaatcagctt gagtagccat ggcgatcgct
     5281 agccctatag tgagtcgtat taatactcta gccttaagag ctgtaattga actgggagtg
     5341 gacacctgtg gagagaaagg caaagtggat gtcagtaaga ccaataggtg cctatcagaa
     5401 acgcaagagt cttctctgtc tcgacaagcc cagtttctat tggtctcctt aaacctgtct
     5461 tgtaaccttg atacttacct gcccagtgcc tcacgaccaa cttctgcagc ttaagttcga
     5521 gactgttgtg tcagaagcac tgactgcgtt agcaatttaa ctgtgataaa ctaccgcaat
     5581 aaagcttcta gtgatctgac ggttcactaa accagctctg cttatataga cctcccaccg
     5641 tacacgccta ccgcccattt gcgtcaacgg ggcggggtta ttacgacatt ttggaaagtc
     5701 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
     5761 tccccgtgag tcaaaccgct atccacgccc attggtgtac tgccaaaacc gcatcaccat
     5821 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc gtaaggtcat
     5881 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcggact
     5941 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     6001 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     6061 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     6121 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     6181 agtggcgcgc cagggaggcc tatatcagca gagctggttt agtgaaccgt cagatcgccc
     6241 atttaggtga cactatagaa tacaagctac ttgttctttt tgcaaccatg gtcttcacac
     6301 tcgaagattt cgttggggac tggcgacaga cagccggcta caacctggac caagtccttg
     6361 aacagggagg tgtgtccagt ttgtttcaga atctcggggt gtccgtaact ccgatccaaa
     6421 ggattgtcct gagcggtgaa aatgggctga agatcgacat ccatgtcatc atcccgtatg
     6481 aaggtctgag cggcgaccaa atgggccaga tcgaaaaaat ttttaaggtg gtgtaccctg
     6541 tggatgatca tcactttaag gtgatcctgc actatggcac actggtaatc gacggggtta
     6601 cgccgaacat gatcgactat ttcggacggc cgtatgaagg catcgccgtg ttcgacggca
     6661 aaaagatcac tgtaacaggg accctgtgga acggcaacaa aattatcgac gagcgcctga
     6721 tcaaccccga cggctccctg ctgttccgag taaccatcaa cggagtgacc ggctggcggc
     6781 tgtgcgaacg cattctggcg ggctcgagcg gcgcgatcgc catgccgcag ctgaacggcg
     6841 gtggagggga tgacctaggc gccaacgacg aactgatttc cttcaaagac gagggcgaac
     6901 aggaggagaa gagctccgaa aactcctcgg cagagaggga tttagctgat gtcaaatcgt
     6961 ctctagtcaa tgaatcagaa acgaatcaaa acagctcctc cgattccgag gcggaaagac
     7021 ggcctccgcc tcgctccgaa agtttccgag acaaatcccg ggaaagtttg gaagaagcgg
     7081 ccaagaggca agatggaggg ctctttaagg ggccaccgta tcccggctac cccttcatca
     7141 tgatccccga cctgacgagc ccctacctcc ccaacggatc gctctcgccc accgcccgaa
     7201 ccctccattt tcagtccggc agcacacatt actctgcgta caaaacgatt gaacaccaga
     7261 ttgcagttca gtatctccag atgaaatggc cactgcttga tgtccaggca gggagcctcc
     7321 agagtagaca agccctcaag gatgcccggt ccccatcacc ggcacacatt gtctctaaca
     7381 aagtgccagt ggtgcagcac cctcaccatg tccaccccct cacgcctctt atcacgtaca
     7441 gcaatgaaca cttcacgccg ggaaacccac ctccacactt accagccgac gtagacccca
     7501 aaacaggaat cccacggcct ccgcaccctc cagatatatc cccgtattac ccactatcgc
     7561 ctggcaccgt aggacaaatc ccccatccgc taggatggtt agtaccacag caaggtcaac
     7621 cagtgtaccc aatcacgaca ggaggattca gacaccccta ccccacagct ctgaccgtca
     7681 atgcttccat gtccaggttc cctccccata tggtcccacc acatcatacg ctacacacga
     7741 cgggcattcc gcatccggcc atagtcacac caacagtcaa acaggaatcg tcccagagtg
     7801 atgtcggctc actccatagt tcaaagcatc aggactccaa aaaggaagaa gaaaagaaga
     7861 agccccacat aaagaaacct cttaatgcat tcatgttgta tatgaaggaa atgagagcaa
     7921 aggtcgtagc tgagtgcacg ttgaaagaaa gcgcggccat caaccagatc cttgggcgga
     7981 ggtggcatgc actgtccaga gaagagcaag cgaaatacta cgagctggcc cggaaggagc
     8041 gacagcttca tatgcaactg taccccggct ggtccgcgcg ggataactat ggaaagaaga
     8101 agaagaggaa aagggacaag cagccgggag agaccaatga acacagcgaa tgtttcctaa
     8161 atccttgcct ttcacttcct ccgattacag acctgagcgc tcctaagaaa tgccgagcgc
     8221 gctttggcct tgatcaacag aataactggt gcggcccttg caggagaaaa aaaaagtgcg
     8281 ttcgctacat acaaggtgaa ggcagctgcc tcagcccacc ctcttcagat ggaagcttac
     8341 tagattcgcc tcccccctcc ccgaacctgc taggctcccc tccccgagac gccaagtcac
     8401 agactgagca gacccagcct ctgtcgctgt ccctgaagcc cgaccccctg gcccacctgt
     8461 ccatgatgcc tccgccaccc gccctcctgc tcgctgaggc cacccacaag gcctccgccc
     8521 tctgtcccaa cggggccctg gacctgcccc cagccgcttt gcagcctgcc gccccctcct
     8581 catcaattgc acagccgtcg acttcttcct tacattccca cagctccctg gccgggaccc
     8641 agccccagcc gctgtcgctc gtcaccaagt ctttagaagt ttaaacgaat tcgggctcgg
     8701 tacccgggga tcctctagaa acacctaggc tctcgtacga agtcagctga taacgtcgac
     8761 gtattgcggc cgcggatcat aatcagccat accacatttg tagaggtttt acttgcttta
     8821 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     8881 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     8941 aataaagcat ttttttcact gccgacgtct taattaacgc gtcttgcgca gcaccatggc
     9001 ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga aagaaccagc
     9061 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     9121 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     9181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     9241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     9301 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     9361 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcgattct tctgacacta
     9421 gcgccaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg
     9481 ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta
     9541 gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag
     9601 aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg
     9661 cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc
     9721 tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag
     9781 ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaaact ctagccatca
     9841 cgatggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
     9901 cgattccgga ctataaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9961 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    10021 cttc
//
