LOCUS       sequence-489002-       10410 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  expresses A. thaliana Cpn60-alpha Cpn60-beta Cpn20 Raf2 RbcX Bsd2 G.
            max Raf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Archer J, Kathpalia M, Lee BU, Li S, Wang T
  TITLE     Effects of chaperone selectivity on the assembly of plant Rubisco 
            orthologs in E. coli.
  JOURNAL   J Exp Bot. 2025 Mar 31:eraf140. doi: 10.1093/jxb/eraf140.
  PUBMED    40163664
REFERENCE   2  (bases 1 to 10410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Mar 31:eraf140. doi: 10.1093/jxb/eraf140."
COMMENT     Sequence Label: pTW334f2
FEATURES             Location/Qualifiers
     source          1..10410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             86..877
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        join(1194..1199,1200..1215,1216..1222)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1230..1246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4545..4567
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             5834..5842
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             6260..6282
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    8004..8020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8028..8034,8035..8050,8051..8056))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        8299..8376
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8377..9459
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8396..8415)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      9616..10354
                     /direction=RIGHT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        join(10404..10410,1..85)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
       61 tcaataatat tgaaaaagga agagtatgag ggaagcggtg atcgccgaag tatcgactca
      121 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      181 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
      241 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      301 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      361 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      421 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      481 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      541 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      601 atggaactcg ccgcccgact gggctggaga tgagcgaaat gtagtgctta cgttgtcccg
      661 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
      721 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
      781 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
      841 cgtgaaaggc gagatcacca aggtagtcgg caaataaacg ccatgggcat taaaaaatgc
      901 cctcttgggt tatcaagagg gtcattatat ttgctgagga gtgccgttaa tgcgcaacgc
      961 aattaatgta agttagctca ctcattaggc acaattctca tgtttgacag cttatcatcg
     1021 actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg
     1081 tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa
     1141 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa
     1201 ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     1261 cagccagtcc gtttaggtgt tttcacgagc acttcaccaa caacgcgtac aactaggaga
     1321 tataccatgg gtatggccgt tcttaactct tcctccttaa aaaacccgaa caaccgttac
     1381 ttccccagca tcaggtctat cagccgttcc gtcctccgcc gtcacctctg ccgtctcagt
     1441 tcggcaccct tgatattgcg ggacgcatcg acatcctggc caaccgctta ggtctgtggt
     1501 atgaatacgc gcccttaatc aattcattaa ttcgagaggg ctttagtcca cctactatcg
     1561 aggaaacaac cggcatttcg ggcgtcgaac agaatcgcct cattgtgggg gcgcaagtgc
     1621 gcgatagctt ggtgcactcg aaagccgatc ctgacctcct ggcagcattt gagacgggcg
     1681 gagccgagct gctctatgag attcggttac tgtcggcttc tcagcgtgtg gcggccgcgc
     1741 gcttcctggt ggaaaatcgc tgcgatggta aagcagccca ggaattagca agaagcatga
     1801 aggattttcc gagccgacgt ggcgataaag gatgggctcg ctttgactac actctgccgg
     1861 gtgattgttt gagctttatg tattaccgtc agtcacgtga acatcgtaac ccgagtgaac
     1921 agcggaccag cgcccttgag caagcgctgc gcgtcgcgga gacggaagcg gctcgcaata
     1981 tgatattgga agaacttgaa gggaacggcg aagagggtga caaagttgat gccggcgaag
     2041 gtgccgttcg cgtaccggta gtgcgtctgc gcattggaga agtggccgaa gcgtcgagtg
     2101 tggtagtact gcccgtaagt gcggcggaag agcgtgaaat tctggaagcg ccgtacgaat
     2161 cccgcagcca aggtgttttt ggtgttgtcg tggcagaaaa aggctgggga aaatgggttg
     2221 tgcttccgag ctgggatccg gttgtgggct tgggtaaagg gggcgtcgtg gtatccttcc
     2281 cggatgcacg cgttctgccg tggaaagtca acaggtggta caaagaagaa ccaatcctag
     2341 tggtcgccga tcgtagcaag aaagaagtag gcgcagacga cggtttctat ctagttaacg
     2401 ctgatggcga gggtctgaaa gttgagcgtg gctcagggtt aaaggaaaaa ggctttaccc
     2461 aatctctcgg caccgttctg ctggtggtgc gtccacccaa ggaagagaat gacgaactgt
     2521 ccgatgaaga ttgggaataa attacaaaaa ttaggagata tacatatgat gtctaatctg
     2581 gcgcaggatt ttcttggtga cttcggagct cgtgaccctt acccggagga gatagcgagt
     2641 caattcggag ataaagtgtt gggatgccaa agcactgagc acaagatttt gataccaaac
     2701 gcatctgttt tgtctctctc ccagcttcag tgttcccctg tttcgtcttc acagcctcct
     2761 ttgtccggcg atgatgccag aactctcctc cacaaggttt tgggatggag tatagtggat
     2821 aatgaagcgg gtggtctgaa aataaggtgt atgtggaaag tgagggattt tgggtgcggt
     2881 gttgaactca taaacaggat ccataaggtt gctgaagctt ctggtcatta cccttctctt
     2941 cacttggaaa gtcctaccca agttcgagct gaactattta cctcttctat cggagggttg
     3001 agcatgaatg atttcataat ggcggctaaa atagatgata tcaagacttc tgatctttcc
     3061 cctaggaaaa gagcttgggc gtgacggctg aaattaatga ggagatatac atatggtatc
     3121 gagaattgcg aatcgaaaga gcaaaaagct cttgattgtg aatgaggatg ttgctggtaa
     3181 ttacgacgat accttcggtg atgtacaaaa gcaaattgtt aattatttta catacaaagc
     3241 tgtgaggacg gttcttcatc agctgtacga aatgaatcct cctcaataca cttggttcta
     3301 taatcatatc ataacaaaca gaccgacgga tggcaaacgt ttcctccgtg ccctcggcaa
     3361 ggagagtcag gagcttgcag aaagagtgat gatcacgcgt cttcacttgt atggcaaatg
     3421 gatcaagaaa tgcgaccatg ggaagatata ccaggaaata tcggatgaaa acttggcgtt
     3481 gatgcgtgaa cgcctgatgg agacagtgat atggccttcc gatgacacaa actcaaggta
     3541 attcagctaa gacactgcac aggagatata catatgatgg ccgcaaacaa taatcctcag
     3601 ggcactaaac caaacagttt agtttgtgca aattgtgaag gggaaggttg tgtggcatgt
     3661 tctcaatgca aaggaggtgg tgtgaatttg attgatcatt tcaatggtca gtttaaagct
     3721 ggtgcactct gttggctttg cagaggtaaa aaggaggtgt tgtgtgggga ctgcaatgga
     3781 gctggtttca ttggtgggtt cttgagcacc ttcgatgagt agagtcagta gggccctaaa
     3841 aaaaaacccc gcccctgaca gggcagggtt ttttttggcg aaaaaacccc gctgaagcgg
     3901 ggttttttgc gtacattggc tccgtctaag aaagtatagc catcacatct gaagctctga
     3961 gggcaatgta gttggaacca tctttgccct tgaagtcgtt accagcatac ttggagtaaa
     4021 gtactgtgct tccggttgat actggtagag gcgtaatttt accttcctcg tctagggaac
     4081 ccggtccaac tgctatcacg gtgccaatag aaggcttctc tttggtagtc tcggttaaca
     4141 acaaccctcc agctgttttc tcctccgcct cagcaacctt aataaagact cggtcattca
     4201 aaggtttgag atctttgatg tcctctgtct caagaatgcc aacaatatca tcttccttga
     4261 ggataagatg cttcacatca ttgaactcca cctcagttcc tgcgtatttg gagtagataa
     4321 tttgtgctcc agtagggaca gtgatatcaa ttttgttctt cccaatagtt cttccttcac
     4381 ccacagcaac gacttcacct ccttgaggtt ttgattgagc agtggatgga agtaagatac
     4441 cacctaaagt cttctcctct gcctccttga tcttcaccaa aactcgatct cccaatggct
     4501 taattgaagt atacttaggg gcaacaacag aggccatatg tatatctcct tcttaaagtt
     4561 aaacaaaatt atttctagag ggcaattgtt atccggtcga cgtcgacctt agtatccgta
     4621 acctgagttg tccattgggt tgccgacggg aactggttca ggttccttga tctcaacaac
     4681 aacgcagtcc gacatcaaga aagtctttgc aaccgaagct gcgtgttcca agcaacatct
     4741 cacaaccttt gttggatcga tgattcctgc agccatcaaa tcctcgtact tgccggttgc
     4801 agcattgtaa ccgaatttca cgttatcgtt ggaaagcacc ttctcgctaa ctacacttcc
     4861 attaacacca gcattcttgg cgattagttt tagggggtaa cttagtgctc ttttaactat
     4921 atccgctcca accttttctt catcattatc aagcgtggct ttaatggcat caaccttgga
     4981 agcaagacga agcagagtgc aaccaccacc aacaacaata ccttcctcga cagcggcctt
     5041 tgttgcatta agagcatctt caactctcaa cttcttctct ttgagttctg tctcagtttg
     5101 tgctccaacc tgaatcacag caactccacc agagagcttt gcaattctct cattgagttt
     5161 ctccttctcg taatcttgct ccgcttgctc aataagattt ttaatctgtg taacacgctt
     5221 tttcactgca tcctgtgtgc ttccatcacc cactatggtc gaggtttcct ttgtgaggac
     5281 aacctttgaa gcattaccaa gaacctcttt tccagctttg tcaagtgaaa gaccaacttc
     5341 ctcacggatc acagttgctc cagttagaat ggcaatatca tcaaggtatt ggctcttgcg
     5401 ctctccaaac cctggagctc tgagagctgc tatcttcagt gtacctctaa gcttgtttac
     5461 aacaagagtc gctaaagctt cttgttcaat gtcttcagca ataattaata ttgggtatcc
     5521 gcctctaatt gcatcctcca aaacaccaac aagatccctt gcattggtaa ttttcttgtc
     5581 aacgagaagt aacttgcaat tgtcgaactc gacggacatt ttctcgctgt ctgtcacaaa
     5641 gtaaggagag atataaccac ggtcaaattg cattccttct acgacgtaga ggttgttctc
     5701 ggcactttta ccctcctcaa gggtaaccac acctttcctg cccactttgc tcattgcttc
     5761 agcaatcata tttccaattt cgtcgttgtt acccgcacta acagctgcaa catctgcaag
     5821 ttcactgtct tcaacctcct tagacatttt cttgagctcg gtaaccaaag cctttgctgt
     5881 cttctcaata cctctggtga tcaaaacagg gtttgcacca gcagctacca ccttgacacc
     5941 ctcagcgata aaaccttgtg caagaacaac agatgttgtt gtaccgtcac cagccaagtc
     6001 attggttttg gcagccgctt gcctcacaag cttagcgcca atgttctcaa ccgggtcttc
     6061 caactcaacc tctcttgcaa cagtcacacc atcattaaca attcgtggtg atccatactt
     6121 gctctcaaga acaacatttc gtcctttggg tccaagtgta acgccaacaa gatctgcgag
     6181 cttgttgaca ccagcttgaa gtctcctaat ggtagtccca tctttgttga aatgtaattc
     6241 ctttgctgcc atatgtatat ctccttctta aagttaaaca aaattatttc tagctcctta
     6301 caccatgagg ccctcaggag cagcagcagc aggagccttg ggtttcggtt tgtcaacaac
     6361 aatggcctga gtggtcagta ccattcctgc tacggaagca gcgttctgta gcgcacatct
     6421 tgtcacttta gccggatcaa tcactccagc ttcgaacaga ttctcgtaag tatcagtcat
     6481 tgcgttgtac ccattctccc aatcgctgaa cataatcttt tccacaacaa cttctccttc
     6541 aactccagcg ttctgagcaa taagagcagc tggtgacagc aatgcctttt gtactatgtc
     6601 agctcccaaa cgttcatcag catcctcaaa agtctcctta atggcgggaa taacagtgga
     6661 gagatgcacc aaagcagcac caccacctgg aactatgcct tcctcgatag cagcaaatgt
     6721 tgcgttcttt gcatcctcga tacgaagctt acggtcctca agctcagttt cagttgctgc
     6781 tccgacttta atgacagcaa cacctccaga tagcttcgca attctctcag cgagcttctc
     6841 tgagtcatac acagaatcag tctcaaatag ttctttcttc agctgagcaa tccgagcttg
     6901 taattcgtcc ttggaagctg catctgcaat gagggtagtc gaatctttgc taatcgtgac
     6961 ttttcgggca atacccaatt gatctatggt tgcgttttca accagtaagc tcatgtccat
     7021 ggctaggtac tcagctcctg tcaagattgc aatatcttga agcatggctt ttcttctttc
     7081 tccaaatcct ggcgctttaa cggcaacaac attgaggaca ccacgaagct tgttcacgac
     7141 aagggtagct aaggcttcac cagtaacatc ctctgcaata atcaacaacg gagcacgaag
     7201 ctgagtggtc ttctccaaaa tagggatgat gtctttgatc gcagtgatct tctgatcagt
     7261 gatcaatacc ctagcattct caaactcagc tagtagtttc tcagggtttg taacaaactg
     7321 aggcgagatg taacctctgt caatctccat cccttcttca acttcgaccg tggtttcaaa
     7381 agaagatgaa gattcaatgg acaaaacacc atcaggtcca actttgtcga tggcatcagc
     7441 aatcattgac cctataaggt catcatttcc agcagagata gaagccacag ctctgatgtc
     7501 atcacgacct ttcacgggcc tagctttctt ctgaagctct tcgatcagac cttgaacagt
     7561 cttatcaatt cccctcttga gtgaaacggg attcgcacca gaagtgacgc tcaacaatcc
     7621 atgtttgatt atctcccgag caaggataga cgcagtggtt gtcccatcac cagcagagtc
     7681 attagtctta ctcgcaacct cacgaataag cgctgcacca gcattttcca tggcattagg
     7741 taactcaata gctctagcaa tggtgactcc atcattcaca accttaggac ttccaaattc
     7801 atccaacaca acattcctcc ctctggggcc aagcgtaaga ccaacacaat cagcaagctt
     7861 atcaatacca gcttgtagag cagctctaga gtgctggtca aaagctattt cctttacatt
     7921 catatgtata tctcctactg cacctattgt tggtgaagtg ctcgtgaaaa cacctaaacg
     7981 gactggctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacat tatacgagcc
     8041 gatgattaat tgtcaacagc tcatttcaga atatttgcca gaaccgttat gatgtcggcg
     8101 caaaaaacat tatccagaac gggagtgcgc cttgagcgac acgaattatg cagtgattta
     8161 cgacctgcac agccatacca cagcttccga tggctgcctg acgccagaag cattggtgca
     8221 ccgtgcagtc gatgataagc tgtcaaacat gagaattgtg cctaatgagt gagctaactt
     8281 acattaattg cgttgcgcga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga
     8341 tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat
     8401 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc
     8461 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt
     8521 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc
     8581 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat
     8641 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa
     8701 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg
     8761 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt
     8821 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga
     8881 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca
     8941 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat
     9001 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa
     9061 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag
     9121 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc
     9181 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc
     9241 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct
     9301 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc
     9361 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9421 ctggcacgac aggtttcccg actggaaagc gggcagtgaa gggtccctaa gtctcctcag
     9481 caaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgaccg gatgtcctct
     9541 tcctcctggg ctgacttcag gtgctacatt tgaagagata aattgcactg aaatctagag
     9601 cggttcagta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctt
     9661 ttgccctgta aacgaaaaaa ccacctgggg aggtggtttg atcgaaggtt aagtcagttg
     9721 gggaactgct taaccgtggt aactggcttt cgcagagcac agcaaccaaa tctgtccttc
     9781 cagtgtagcc ggactttggc gcacacttca agagcaaccg cgtgtttagc taaacaaatc
     9841 ctctgcgaac tcccagttac caatggctgc tgccagtggc gttttaccgt gcttttccgg
     9901 gttggactca agtgaacagt taccggataa ggcgcagcag tcgggctgaa cggggagttc
     9961 ttgcttacag cccagcttgg agcgaacgac ctacaccgag ccgagatacc agtgtgtgag
    10021 ctatgagaaa gcgccacact tcccgtaagg gagaaaggcg gaacaggtat ccggtaaacg
    10081 gcagggtcgg aacaggagag cgcaagaggg agcgacccgc cggaaacggt ggggatcttt
    10141 aagtcctgtc gggtttcgcc cgtactgtca gattcatggt tgagcctcac ggctcccaca
    10201 gatgcaccgg aaaagcgtct gtttatgtga actctggcag gagggcggag cctatggaaa
    10261 aacgccaccg gcgcggccct gctgttttgc ctcacatgtt agtcccctgc ttatccacgg
    10321 aatctgtggg taactttgta tgtgtccgca gcgcccgccg cagtctcacg cccggagcgt
    10381 agcgaccgag tgacggcctt aatttggctt
//
