LOCUS       sequence-489023-       10184 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Lentiviral vector with nuclear fluorescent reporter AcGFP expressed 
            from Rat TAC1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Delzor A, Dufour N, Petit F, Guillermier M, Houitte D, Auregan G, 
            Brouillet E, Hantraye P, Deglon N
  TITLE     Restricted transgene expression in the brain with cell-type specific
            neuronal promoters.
  JOURNAL   Hum Gene Ther Methods. 2012 Aug;23(4):242-54. doi: 
            10.1089/hgtb.2012.073. Epub 2012 Aug 30.
  PUBMED    22934828
REFERENCE   2  (bases 1 to 10184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/hgtb.2012.073"; journalName: "Hum Gene Ther Methods"; date:
            "2012-08"; volume: "23"; issue: "4"; pages: "242-54"
COMMENT     Sequence Label: SIN-rTAC1-AcGFPnuc-WPRE
FEATURES             Location/Qualifiers
     source          1..10184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2069..2093
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3001..3025)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    3060..3084
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3143..3152
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3149..3865
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     CDS             3890..3910
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3914..3934
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3938..3958
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(3992..4016)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4039..4055)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4040..4056)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    4069..4657
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4122..4142)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4540..4551)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4746..4979
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5004..5022)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5005..5022)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5312..5331)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5545..5564)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5664..5686
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5724..5742)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5810..5914
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5915..5983,5984..6775)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6133..6152)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6946..7534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7435..7454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7688..7705
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7775..7795)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7780..8109
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7960..8095
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9243..9308
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9438..9458
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9883..10017
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9920..9939)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9974..9993
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgataa gcttgatatc gaattatcac aagtttgtac aaaaaagcag gctccgcggc
     2101 cgcccccttc accacctgca gagctccaaa ggtaagcatc cagcctttct agtcccccaa
     2161 caaggctaaa ggggagagag gcacaattat cctcttccca ccccttctgc cttcagggtg
     2221 tgcctgggaa gaagctgtag gggaacaaaa gatgccttag aatggctgat gggtaagttc
     2281 tacatgagaa aggaggttta aattcctctt tcccctaaat gtaaaacaaa cctgccttca
     2341 tcctctgaag cgggagaccg gaaacacttt tgcagtgcta gagaaatgag aatattctga
     2401 ctgatttggt ggggaggggg gttggggggg tgctgttcca gccctagata taacacctca
     2461 taaaccttaa gacacataaa gtagaaatga aaggaaaacc ccgcttgctt catccctctg
     2521 aagtgcttgc tggtgtctta gtattattca caaggttttg ctgctcaagt tatttggctg
     2581 tcctcaaagc gcaatattcc ctgatgcctc ttgagagaaa agttccctaa gtccgaagca
     2641 tgagtcactt cgctcagttt tgatgagtaa tctcaggtgt cactgaacct tgttcggaag
     2701 aagaggggag gggggcgtca gatttgcaga cggaagaaaa caggtctctc tggattggat
     2761 ggcgagacct cgacttccct aaaattgcgt catttcgaac ccaatttggt ccagatgtta
     2821 tggactccga cgggttaccg tctcggaaac tctatcacgc aagcaaaagg cgagggggcg
     2881 gctaattaaa tattgagcag aaagtcgcgt ggggagagtg tcacgtggct ctccaggctc
     2941 atcacgcctg agataaataa ggcgaagcag gagcagggac tagaaagggt gggcgcgccg
     3001 acccagcttt cttgtacaaa gtggtgataa ttcctgcagc ccgggctgca ggaattatca
     3061 caagtttgta caaaaaagca ggctccacca tgggaaccaa ttcagtcgac tggatccggt
     3121 accgaatcta gcgctaccgg tcgccaccat ggtgagcaag ggcgccgagc tgttcaccgg
     3181 catcgtgccc atcctgatcg agctgaatgg cgatgtgaat ggccacaagt tcagcgtgag
     3241 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3301 cggcaagctg cctgtgccct ggcccaccct ggtgaccacc ctgagctacg gcgtgcagtg
     3361 cttctcacgc taccccgatc acatgaagca gcacgacttc ttcaagagcg ccatgcctga
     3421 gggctacatc caggagcgca ccatcttctt cgaggatgac ggcaactaca agtcgcgcgc
     3481 cgaggtgaag ttcgagggcg ataccctggt gaatcgcatc gagctgaccg gcaccgattt
     3541 caaggaggat ggcaacatcc tgggcaataa gatggagtac aactacaacg cccacaatgt
     3601 gtacatcatg accgacaagg ccaagaatgg catcaaggtg aacttcaaga tccgccacaa
     3661 catcgaggat ggcagcgtgc agctggccga ccactaccag cagaataccc ccatcggcga
     3721 tggccctgtg ctgctgcccg ataaccacta cctgtccacc cagagcgccc tgtccaagga
     3781 ccccaacgag aagcgcgatc acatgatcta cttcggcttc gtgaccgccg ccgccatcac
     3841 ccacggcatg gatgagctgt acaagtccgg actcagatct cgagctgatc caaaaaagaa
     3901 gagaaaggta gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtagg
     3961 atcaattcgc ggccgcactc gagatatcta gacccagctt tcttgtacaa agtggtgata
     4021 attcgatatc aagcttatcg ataccgtcga cctcgaggga attccgataa tcaacctctg
     4081 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     4141 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     4201 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4261 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4321 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4381 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4441 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     4501 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4561 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4621 cagacgagtc ggatctccct ttgggccgcc tccccgcatc gggaattcga gctcggtacc
     4681 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     4741 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     4801 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4861 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4921 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4981 catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg tatgatacat
     5041 aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc taattgtgta
     5101 gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt cagagtcggt
     5161 ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga aatggtcagc
     5221 gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat cgtggccggc
     5281 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
     5341 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     5401 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
     5461 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
     5521 gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     5581 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5641 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     5701 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     5761 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     5821 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5881 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5941 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     6001 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     6061 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6121 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6181 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6241 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6301 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6361 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6421 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     6481 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6541 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6601 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6661 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6721 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6781 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6841 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6901 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6961 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7021 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7081 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7141 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7201 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7261 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7321 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7381 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7441 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7501 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7561 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7621 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7681 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     7741 ccccgcgcgt tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga
     7801 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     7861 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7921 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     7981 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8041 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8101 ttttgcaaaa agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta
     8161 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
     8221 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
     8281 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
     8341 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
     8401 cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt
     8461 attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt
     8521 aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg
     8581 gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc
     8641 atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa
     8701 gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt
     8761 cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt
     8821 acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga
     8881 aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa
     8941 tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta
     9001 tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga
     9061 cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac
     9121 ggtgattggc taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg
     9181 aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta
     9241 aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     9301 tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg
     9361 aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact
     9421 ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt
     9481 cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg
     9541 ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt
     9601 ctgtaacctt tataagtagg cataacagtt ataatcataa catactgttt tttcttactc
     9661 cacacaggca tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct
     9721 ttttaatttg taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc
     9781 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     9841 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     9901 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     9961 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac
    10021 tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact
    10081 gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt
    10141 taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
