LOCUS       sequence-489026-        9908 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  Lentiviral vector with nuclear fluorescent reporter AcGFP expressed 
            from Mouse D1R promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9908)
  AUTHORS   Delzor A, Dufour N, Petit F, Guillermier M, Houitte D, Auregan G, 
            Brouillet E, Hantraye P, Deglon N
  TITLE     Restricted transgene expression in the brain with cell-type specific
            neuronal promoters.
  JOURNAL   Hum Gene Ther Methods. 2012 Aug;23(4):242-54. doi: 
            10.1089/hgtb.2012.073. Epub 2012 Aug 30.
  PUBMED    22934828
REFERENCE   2  (bases 1 to 9908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/hgtb.2012.073"; journalName: "Hum Gene Ther Methods"; date:
            "2012-08"; volume: "23"; issue: "4"; pages: "242-54"
COMMENT     Sequence Label: SIN-mD1R promoter-AcGFPnuc-WPRE
FEATURES             Location/Qualifiers
     source          1..9908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     protein_bind    2069..2093
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2784..2808
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2867..2876
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2873..3589
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     CDS             3614..3634
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3638..3658
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3662..3682
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(3716..3740)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(3763..3779)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3764..3780)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    3793..4381
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3846..3866)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4264..4275)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4470..4703
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4728..4746)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4729..4746)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5036..5055)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5269..5288)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5388..5410
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5448..5466)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5534..5638
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5639..5707,5708..6499)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5857..5876)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6670..7258
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7159..7178
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7412..7429
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7499..7519)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7504..7833
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7684..7819
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8967..9032
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9162..9182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9607..9741
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9644..9663)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9698..9717
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgataa gcttgatatc gaattatcac aagtttgtac aaaaaagcag gctccgcggc
     2101 cgcccccttc accatcgata acgctgcctg tcaagcgagg accgccccca gggcagggaa
     2161 gcttctggaa cccagccggc cggtgccctc gctcgctcga gctgttgtgc ttgcctggag
     2221 ctcctgccac tgctgacagg gagcgagcgg gtggctcccc aaggaggctc agggtcctgc
     2281 cctaagaacg aggagccaaa ggggtgccca gagctgggct tcctcagaca ggacagaagc
     2341 tgttctgcag taggtggtct tgatggactt gtccaggggg tgatgggctg agattttgtg
     2401 tagccaaata ggaggtctgc acagagtatt ggtgaacgac tttgtgtttc cctttgcttt
     2461 ttagggcatt tggagagatg tggcaccagg ggcttgaagg agaaatgcat gtattttagg
     2521 ctgtgtctca gaagaagagg cagcatccct gaaaagtgac tgagattgac caggaagagg
     2581 ccccagactc ttagagtatg cttaaaagcc tatgcattcc ttggggaatc tagggaccag
     2641 cggatgccag ggaacagcga cctggagcac tgaacccaga agacaggtgg aaagcaagcg
     2701 gctcttctgg atcaaactta agcttggtac cgagctcgga tcttccctag aactagtgga
     2761 tcccccgggc tgcaggaatt atcacaagtt tgtacaaaaa agcaggctcc accatgggaa
     2821 ccaattcagt cgactggatc cggtaccgaa tctagcgcta ccggtcgcca ccatggtgag
     2881 caagggcgcc gagctgttca ccggcatcgt gcccatcctg atcgagctga atggcgatgt
     2941 gaatggccac aagttcagcg tgagcggcga gggcgagggc gatgccacct acggcaagct
     3001 gaccctgaag ttcatctgca ccaccggcaa gctgcctgtg ccctggccca ccctggtgac
     3061 caccctgagc tacggcgtgc agtgcttctc acgctacccc gatcacatga agcagcacga
     3121 cttcttcaag agcgccatgc ctgagggcta catccaggag cgcaccatct tcttcgagga
     3181 tgacggcaac tacaagtcgc gcgccgaggt gaagttcgag ggcgataccc tggtgaatcg
     3241 catcgagctg accggcaccg atttcaagga ggatggcaac atcctgggca ataagatgga
     3301 gtacaactac aacgcccaca atgtgtacat catgaccgac aaggccaaga atggcatcaa
     3361 ggtgaacttc aagatccgcc acaacatcga ggatggcagc gtgcagctgg ccgaccacta
     3421 ccagcagaat acccccatcg gcgatggccc tgtgctgctg cccgataacc actacctgtc
     3481 cacccagagc gccctgtcca aggaccccaa cgagaagcgc gatcacatga tctacttcgg
     3541 cttcgtgacc gccgccgcca tcacccacgg catggatgag ctgtacaagt ccggactcag
     3601 atctcgagct gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtaga
     3661 tccaaaaaag aagagaaagg taggatcaat tcgcggccgc actcgagata tctagaccca
     3721 gctttcttgt acaaagtggt gataattcga tatcaagctt atcgataccg tcgacctcga
     3781 gggaattccg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3841 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3901 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3961 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4021 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4081 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4141 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     4201 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4261 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4321 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4381 catcgggaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct
     4441 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     4501 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     4561 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     4621 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     4681 gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac
     4741 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac
     4801 aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc
     4861 tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc
     4921 gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc
     4981 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
     5041 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
     5101 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
     5161 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
     5221 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc
     5281 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     5341 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     5401 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     5461 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5521 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5581 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5641 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5701 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5761 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5821 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5881 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5941 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6001 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6061 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6121 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6181 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6241 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6301 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6361 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6421 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6481 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6541 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6601 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6661 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6721 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6781 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     6841 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6901 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6961 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7021 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7081 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7141 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7201 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7261 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7321 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7381 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7441 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgtgg
     7501 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7561 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     7621 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     7681 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7741 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7801 ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac aggcttgcga
     7861 gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc
     7921 ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat
     7981 tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga
     8041 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact
     8101 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt
     8161 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
     8221 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
     8281 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg
     8341 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
     8401 gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga tatgggcgtc
     8461 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt
     8521 gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc
     8581 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
     8641 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga
     8701 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
     8761 cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg
     8821 taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact ggatttatga
     8881 gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac
     8941 tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta
     9001 aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg
     9061 agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta
     9121 gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg
     9181 tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta
     9241 gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat
     9301 acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac agttataatc
     9361 ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt aataactatg
     9421 ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag gaatatttga
     9481 tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga ggttttactt
     9541 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     9601 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9661 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9721 gtatcttatc atgtctggat caactggata actcaagcta accaaaatca tcccaaactt
     9781 cccaccccat accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt
     9841 gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac
     9901 ttagtagt
//
