LOCUS       sequence-489092-        8267 bp    DNA     circular SYN 09-OCT-2025
DEFINITION  CBh promoter expression plasmid for C-terminal intein-split AAV 
            construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8267)
  TITLE     Kleinstiver paper in preparation 2025
REFERENCE   2  (bases 1 to 8267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 9, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)
FEATURES             Location/Qualifiers
     source          1..8267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        complement(14..89)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      complement(103..130)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(146..217)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(235..912)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             919..930
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    948..966
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    973..991
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(999..1247)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(1057..1076)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(1227..1247)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     repeat_region   1271..1400
                     /label=AAV2 ITR
     primer_bind     complement(1419..1436)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1419..1435)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1428..1450)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1577..2032
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1664..1683)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1874..1895
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2058..2162
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2163..2231,2232..3023)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2381..2400)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3194..3782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3683..3702
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    4070..4091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4106..4111,4112..4129,4130..4136)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4144..4160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4149..4171
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4168..4184
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4168..4184
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   4211..4340
                     /label=AAV2 ITR
     enhancer        4363..4648
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        4650..4927
                     /label=chicken beta-actin promoter
     intron          4928..5155
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             5209..5229
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5335..7719
                     /codon_start=1
                     /product="C-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(C)
                     /translation="CFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILED
                     IVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGK
                     TILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKG
                     ILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQI
                     LKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDN
                     KVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDK
                     AGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMP
                     QVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKV
                     EKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
                     GRKRMLASARELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLD
                     EIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKY
                     FDTTIDRKQYRSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     CDS             7762..7782
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(7847..7867)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    8043..8267
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctcgagaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg actagcctta
       61 ttttaacttg ctatttctag ctctaaaacc gagacgtata aacgcagaaa ggcccacccg
      121 aaggtgagcc agtgtgactc tagtagagag cgttcaccga caaacaacag ataaaacgaa
      181 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggagatc cttattaagc
      241 acccgtggag tgacgacctt cagcacgttc gtactgttca acgatggtgt agtcttcgtt
      301 gtgggaggtg atgtccagtt tgatgtcggt tttgtaagca cccggcagct gaacgggttt
      361 tttagccatg taggtggttt taacttcagc gtcgtagtga ccaccgtctt tcagtttcag
      421 acgcattttg atttcacctt tcagagcacc gtcttccggg tacatacgtt cggtggaagc
      481 ttcccaaccc atggtttttt tctgcataac cggaccgtcg gacgggaagt tggtaccacg
      541 cagtttaact ttgtagatga actcaccgtc ttgcagggag gagtcctggg taacggtaac
      601 aacaccaccg tcttcgaagt tcataacacg ttcccatttg aaaccttccg ggaaggacag
      661 tttcaggtag tccgggatgt cagccgggtg tttaacgtaa gctttggaac cgtactggaa
      721 ctgcggggac aggatgtccc aagcgaacgg cagcggacca cctttggtaa ctttcagttt
      781 agcggtctgg gtaccttcgt acggacgacc ttcaccttca ccttcgattt cgaactcgtg
      841 accgttaacg gaaccttcca tacgaacttt gaaacgcatg aactctttga taacgtcttc
      901 gctactcgcc atagatcctt tctcctcttt cagatccgtg ctcagtatct ctatcactga
      961 tagggatgtc aatctctatc actgataggg acgtctcggg tgtttcgtcc tttccacaag
     1021 atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt
     1081 ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta
     1141 ttttgtacta atatctttgt gtttacagtc aaattaattc taattatctc tctaacagcc
     1201 ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctcttc ctgcccgacc
     1261 ttgcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     1321 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     1381 gtgagcgagc gagcgcgcag ccttaattaa cctaattcac tggccgtcgt tttacaacgt
     1441 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     1501 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     1561 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     1621 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     1681 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     1741 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     1801 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     1861 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     1921 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     1981 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     2041 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2101 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2161 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     2221 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     2281 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     2341 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     2401 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     2461 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     2521 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     2581 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     2641 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     2701 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     2761 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     2821 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     2881 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2941 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     3001 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     3061 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     3121 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     3181 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     3241 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     3301 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     3361 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     3421 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     3481 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3541 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     3601 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3661 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3721 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3781 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3841 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3901 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3961 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     4021 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     4081 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     4141 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccagat
     4201 ttaattaagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg
     4261 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa
     4321 ctccatcact aggggttcct gcggcctcta gatcagggta cccgttacat aacttacggt
     4381 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc
     4441 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     4501 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     4561 gcccgcctgg cattgtgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     4621 ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct
     4681 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
     4741 tgcagcgatg ggggcggggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga
     4801 ggggcggggc ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg
     4861 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     4921 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
     4981 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     5041 ttctcctccg ggctgtaatt agctgagcaa gaggtaaggg tttaagggat ggttggttgg
     5101 tggggtatta atgtttaatt acctggagca cctgcctgaa atcacttttt ttcaggttgg
     5161 accggtgcca ccatgaaacg gacagccgac ggaagcgagt tcgagtcacc aaagaagaag
     5221 cggaaagtca tcaagattgc tacacggaaa tacctgggaa agcagaacgt gtacgacatc
     5281 ggcgtggagc gggatcacaa cttcgccctg aagaatggct ttatcgccag caattgcttc
     5341 gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac
     5401 gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt
     5461 ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg
     5521 ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga
     5581 tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc
     5641 ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag
     5701 ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc
     5761 cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag
     5821 ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag
     5881 cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag
     5941 aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc
     6001 ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac
     6061 ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac
     6121 tacgatgtgg accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag
     6181 gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc
     6241 gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga
     6301 aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc
     6361 ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg
     6421 gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg
     6481 atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg
     6541 cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc
     6601 gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg
     6661 tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag
     6721 tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc
     6781 gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat
     6841 aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg
     6901 aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac
     6961 agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgtg
     7021 agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag
     7081 aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag
     7141 aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc
     7201 atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc
     7261 tctgccagag aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc
     7321 ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa
     7381 cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag
     7441 ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac
     7501 aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg
     7561 accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagcag
     7621 tacagaagca ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg
     7681 tacgagacac ggatcgacct gtctcagctg ggaggtgact ctggcggctc aaaaagaacc
     7741 gccgacggca gcgaattcga gcccaagaag aagaggaaag tctaagatct gataatcaac
     7801 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     7861 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7921 tcattttctc ctccttgtat aaatcctggt tagttcttgc cacggcggaa ctcatcgccg
     7981 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgc
     8041 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     8101 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     8161 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     8221 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatgg
//
