LOCUS       sequence-489100-        9703 bp    DNA     circular SYN 10-OCT-2025
DEFINITION  Lentiviral vector with nuclear fluorescent reporter AcGFP expressed 
            from Mouse PGK promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9703)
  AUTHORS   Delzor A, Dufour N, Petit F, Guillermier M, Houitte D, Auregan G, 
            Brouillet E, Hantraye P, Deglon N
  TITLE     Restricted transgene expression in the brain with cell-type specific
            neuronal promoters.
  JOURNAL   Hum Gene Ther Methods. 2012 Aug;23(4):242-54. doi: 
            10.1089/hgtb.2012.073. Epub 2012 Aug 30.
  PUBMED    22934828
REFERENCE   2  (bases 1 to 9703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/hgtb.2012.073"; journalName: "Hum Gene Ther Methods"; date:
            "2012-08"; volume: "23"; issue: "4"; pages: "242-54"
COMMENT     Sequence Label: SIN-mPGK-AcGFPnuc-WPRE
FEATURES             Location/Qualifiers
     source          1..9703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2090..2589
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2125..2148)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2522..2541
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     protein_bind    2609..2633
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2692..2701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2698..3414
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     CDS             3439..3459
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3463..3483
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3487..3507
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(3541..3565)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3585..4173
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3638..3658)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4056..4067)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4262..4495
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4523..4541)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4524..4541)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4831..4850)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5064..5083)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5183..5205
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5243..5261)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5329..5433
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5434..5502,5503..6294)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5652..5671)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6465..7053
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6954..6973
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7207..7224
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7294..7314)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7299..7628
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7479..7614
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8762..8827
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8957..8977
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9402..9536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9439..9458)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9493..9512
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgatgg ccatcgatgg ccatcgatgg ccatcgatgg tcgagtaccg ggtaggggag
     2101 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
     2161 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
     2221 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
     2281 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
     2341 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
     2401 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
     2461 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg
     2521 gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg
     2581 ggcctttcga cctctagatc ggatcatcac aagtttgtac aaaaaagcag gctccaccat
     2641 gggaaccaat tcagtcgact ggatccggta ccgaatctag cgctaccggt cgccaccatg
     2701 gtgagcaagg gcgccgagct gttcaccggc atcgtgccca tcctgatcga gctgaatggc
     2761 gatgtgaatg gccacaagtt cagcgtgagc ggcgagggcg agggcgatgc cacctacggc
     2821 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ctgtgccctg gcccaccctg
     2881 gtgaccaccc tgagctacgg cgtgcagtgc ttctcacgct accccgatca catgaagcag
     2941 cacgacttct tcaagagcgc catgcctgag ggctacatcc aggagcgcac catcttcttc
     3001 gaggatgacg gcaactacaa gtcgcgcgcc gaggtgaagt tcgagggcga taccctggtg
     3061 aatcgcatcg agctgaccgg caccgatttc aaggaggatg gcaacatcct gggcaataag
     3121 atggagtaca actacaacgc ccacaatgtg tacatcatga ccgacaaggc caagaatggc
     3181 atcaaggtga acttcaagat ccgccacaac atcgaggatg gcagcgtgca gctggccgac
     3241 cactaccagc agaatacccc catcggcgat ggccctgtgc tgctgcccga taaccactac
     3301 ctgtccaccc agagcgccct gtccaaggac cccaacgaga agcgcgatca catgatctac
     3361 ttcggcttcg tgaccgccgc cgccatcacc cacggcatgg atgagctgta caagtccgga
     3421 ctcagatctc gagctgatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag
     3481 gtagatccaa aaaagaagag aaaggtagga tcaattcgcg gccgcactcg agatatctag
     3541 acccagcttt cttgtacaaa gtggtgattc gagggaattc cgataatcaa cctctggatt
     3601 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3661 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3721 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3781 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3841 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3901 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3961 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     4021 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     4081 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4141 cgagtcggat ctccctttgg gccgcctccc cgcatcggga attcgagctc ggtaccttta
     4201 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4261 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     4321 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4381 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     4441 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc
     4501 tagtagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata
     4561 aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag
     4621 catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt
     4681 tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg
     4741 aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca
     4801 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     4861 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     4921 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     4981 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     5041 agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     5101 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     5161 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     5221 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     5281 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     5341 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5401 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5461 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5521 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5581 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5641 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5701 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5761 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5821 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5881 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5941 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6001 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6061 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6121 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6181 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6241 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6301 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6361 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6421 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6481 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6541 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6601 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6661 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6721 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6781 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6841 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6901 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6961 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7021 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7081 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7141 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7201 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7261 cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa
     7321 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7381 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7441 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7501 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7561 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7621 tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat
     7681 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
     7741 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
     7801 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
     7861 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
     7921 attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta
     7981 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
     8041 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
     8101 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
     8161 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
     8221 atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
     8281 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta
     8341 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
     8401 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
     8461 cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat
     8521 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac
     8581 gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg
     8641 gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga
     8701 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
     8761 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
     8821 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
     8881 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
     8941 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
     9001 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
     9061 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
     9121 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
     9181 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
     9241 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
     9301 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     9361 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     9421 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     9481 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
     9541 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
     9601 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
     9661 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
