LOCUS       sequence-489110-        7372 bp    DNA     circular SYN 10-OCT-2025
DEFINITION  Expresses soma-targeted (ribosome tethered via RiboL1) jGCaMP8m 
            under CaMKII promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Grodem S, Nymoen I, Vatne GH, Rogge FS, Bjornsdottir V, Lensjo KK, 
            Fyhn M
  TITLE     An updated suite of viral vectors for in vivo calcium imaging using 
            intracerebral and retro-orbital injections in male mice.
  JOURNAL   Nat Commun. 2023 Feb 4;14(1):608. doi: 10.1038/s41467-023-36324-3.
  PUBMED    36739289
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-02-4"; volume: "14(1):608. doi"; pages: " 
            10.1038/s41467-023-36324-3"
COMMENT     Sequence Label: pAAV CaMKII:RiboL1-jGCaMP8m
FEATURES             Location/Qualifiers
     source          1..7372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   651..780
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        803..2091
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             2114..2131
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     2413..2434
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2540..2561)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2801..2820)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    4149..4737
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4202..4222)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4620..4631)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4769..5245
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4968..4987)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5285..5425
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5285..5414
                     /label=AAV2 ITR
     rep_origin      5500..5955
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5587..5606)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5797..5818
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5972..5991)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6091..6113
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6151..6169)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6237..6341
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6342..6410,6411..7202)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6560..6579)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
      661 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
      721 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
      781 gcggccgcac gcgtttaatt aacattatgg ccttaggtca cttcatctcc atggggttct
      841 tcttctgatt ttctagaaaa tgagatgggg gtgcagagag cttcctcagt gacctgccca
      901 gggtcacatc agaaatgtca gagctagaac ttgaactcag attactaatc ttaaattcca
      961 tgccttgggg gcatgcaagt acgatataca gaaggagtga actcattagg gcagatgacc
     1021 aatgagttta ggaaagaaga gtccagggca gggtacatct acaccacccg cccagccctg
     1081 ggtgagtcca gccacgttca cctcattata gttgcctctc tccagtccta ccttgacggg
     1141 aagcacaagc agaaactggg acaggagccc caggagacca aatcttcatg gtccctctgg
     1201 gaggatgggt ggggagagct gtggcagagg cctcaggagg ggccctgctg ctcagtggtg
     1261 acagataggg gtgagaaagc agacagagtc attccgtcag cattctgggt ctgtttggta
     1321 cttcttctca cgctaaggtg gcggtgtgat atgcacaatg gctaaaaagc agggagagct
     1381 ggaaagaaac aaggacagag acagaggcca agtcaaccag accaattccc agaggaagca
     1441 aagaaaccat tacagagact acaaggggga agggaaggag agatgaatta gcttcccctg
     1501 taaaccttag aacccagctg ttgccagggc aacggggcaa tacctgtctc ttcagaggag
     1561 atgaagttgc cagggtaact acatcctgtc tttctcaagg accatcccag aatgtggcac
     1621 ccactagccg ttaccatagc aactgcctct ttgccccact taatcccatc ccgtctgtta
     1681 aaagggccct atagttggag gtgggggagg taggaagagc gatgatcact tgtggactaa
     1741 gtttgttcgc atccccttct ccaaccccct cagtacatca ccctggggga acagggtcca
     1801 cttgctcctg ggcccacaca gtcctgcagt attgtgtata taaggccagg gcaaagagga
     1861 gcaggtttta aagtgaaagg caggcaggtg ttggggaggc agttaccggg gcaacgggaa
     1921 cagggcgttt cggaggtggt tgccatgggg acctggatgc tgacgaaggc tcgcgaggct
     1981 gtgagcagcc acagtgccct gctcagaagc cccaagctcg tcagtcaagc cggttctccg
     2041 tttgcactca ggagcacggg caggcgagtg gcccctagtt ctgggggcag cgggggatcc
     2101 acccgccacc atgcatcatc accatcatca cacgcgtcgc aagaagacct tcaaggaggt
     2161 ggccaccgcc gtgaagatca tcggccgcct gatgggcctg aagatcaacg tctatatcaa
     2221 ggccgacaag cagaagaacg gcatcaaggc gaacttccac atccgccaca acatcgagga
     2281 cggcggcgtg cagctcgcct accactacca gcagaacacc cccatcggcg acggccccgt
     2341 gctgctgccc gacaaccact acctgagcgt ggagtccaaa ctttcgaaag accccaacga
     2401 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2461 ggacgagctg tacaagggcg gtaccggagg gagcatggtg agcaagggcg aggagctgtt
     2521 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     2581 cgtgtccggc gagggtgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     2641 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     2701 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     2761 gcccgaaggc tacatccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     2821 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     2881 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaacc tgccggacca
     2941 actgactgaa gagcagatcg cagaatacaa agaggctttc tccctatttg acaaggacgg
     3001 ggatgggaca ataacaacca aggagctggg gacggtgatg cggtctctgg ggcagaaccc
     3061 cacagaagca gagctgcagg acatgatcaa tgaagtagat gccgacggtg acggcacaat
     3121 cgacttccct gagttcctga caatgatggc aagaaaaatg aaatacaggg acacggaaga
     3181 agaaattaga gaagcgttcg gtgtgtttga taaggatggc aatggctaca tcagtgcagc
     3241 agagcttcgc cacgtgatga caaaccttgg agagaagtta acagatgaag aggttgatga
     3301 aatgatcagg gaagcagaca tcgatgggga tggtcaggta aactacgaag agtttgtaca
     3361 aatgatgaca gcgaaggggg gcagcggtgg gtctggaggg actgggggta gcggtggtag
     3421 cggtgggaca gggggaagcg gcggtagtgg tggaacaagc agcaaagtct cacgcgacac
     3481 cctgtacgag gcggtgcggg aagtcctgca cgggaaccag cgcaagcgcc gcaagtttct
     3541 ggagacggtg gagctgcaga tcagcctgaa gaactacgac cctcagaagg acaaacgttt
     3601 ctcgggcacc gtcaggctca agtccacccc acgccccaag ttctcggtgt gcgttctggg
     3661 ggaccagcag cactgtgatg aagccaaggc cgtggatatc ccccacatgg acatcgaggc
     3721 gctcaagaaa cttaacaaaa acaagaagtt ggtcaagaag ctggctaaga agtacgatgc
     3781 ctttttggcc tctgagtctc tgattaagca gatcccacgt atcctgggcc caggcctaaa
     3841 caaggctggc aagttcccct ccctgctgac acacaatgaa aacatggtgg ccaaagtgga
     3901 tgaggtgaaa tcgacaatca agttccagat gaagaaggtg ctgtgtttgg ccgtcgctgt
     3961 tggccacgtg aagatgaccg atgatgagct agtctacaac attcatctgg ctgtcaattt
     4021 cttggtgtcc ttgcttaaga aaaactggca aaacgtgcgg gctctgtaca tcaagagcac
     4081 catgggcaag ccccagcgtc tgtattaggg cgcgcccctg cagggaattc gatatcaagc
     4141 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4201 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4261 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4321 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     4381 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     4441 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     4501 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     4561 ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     4621 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     4681 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     4741 gataccgagc gctgctcgag agatctacgg gtggcatccc tgtgacccct ccccagtgcc
     4801 tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa taaaattaag
     4861 ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga ggggggtggt
     4921 atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct attgggaacc
     4981 aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct gggttcaagc
     5041 gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga ccaggctcag
     5101 ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc tggtctccaa
     5161 ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat tacaggcgtg
     5221 aaccactgct cccttccctg tccttctgat tttgtaggta accacgtgcg gaccgagcgg
     5281 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     5341 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     5401 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
     5461 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     5521 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
     5581 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5641 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5701 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5761 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5821 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     5881 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     5941 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     6001 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     6061 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     6121 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6181 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     6241 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     6301 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     6361 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     6421 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     6481 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6541 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6601 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6661 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6721 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6781 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6841 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6901 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6961 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7021 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7081 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     7141 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     7201 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     7261 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     7321 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tc
//
