LOCUS       sequence-489138-       13678 bp    DNA     circular SYN 10-OCT-2025
DEFINITION  Stably expresses a chimeric protein, 
            SUPV3L1(1-114aa)-ALDH18A1(106aa-385aa)-SUPV3L1(351-786aa).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13678)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 13678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJL192
FEATURES             Location/Qualifiers
     source          1..13678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2610..3198
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             2716..2727
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3125..3145
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3223..3825)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(3309..3329)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     3806..3825
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(3837..4337)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3900..3918)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        4424..5602
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4655..5593
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5550..5570
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     LTR             9483..9663
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9689..9706)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9695..9919
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9965..10393
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10052..10071)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10262..10283
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10402..10422)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10407..10736
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10587..10722
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10649..10668
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10784..10831
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10850..11224
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11354..11475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11391..11410)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11445..11464
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11524..11540)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11524..11540)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11537..11559)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11548..11564
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11572..11578,11579..11596,11597..11602))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11617..11638
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11755..11772)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11926..12514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12006..12025)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12685..13476,13477..13545))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13308..13327
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13546..13650)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagcgatg cggggaggcg gcccaaaggg agatccgact
     2641 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     2701 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     2761 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     2821 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     2881 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     2941 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     3001 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     3061 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     3121 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     3181 gtaatccaga ggttgattac cgataagctt gatatcgaat tcttaactgg caccgggctt
     3241 gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg tgacggtgaa
     3301 gccgagccgc tcgtagaagg ggaggttgcg gggcgcggag gtctccagga aggcgggcac
     3361 cccggcgcgc tcggccgcct ccactccggg gagcacgacg gcgctgccca gacccttgcc
     3421 ctggtggtcg ggcgagacgc cgacggtggc caggaaccac gcgggctcct tgggccggtg
     3481 cggcgccagg aggccttcca tctgttgctg cgcggccagc cgggaaccgc tcaactcggc
     3541 catgcgcggg ccgatctcgg cgaacaccgc ccccgcttcg acgctctccg gcgtggtcca
     3601 gaccgccacg gcggcgccgt cgtccgcgac ccacaccttg ccgatgtcga gcccgacgcg
     3661 cgtgaggaag agttcttgca gctcggtgac ccgctcgatg tggcggtccg gatcgacggt
     3721 gtggcgcgtg gcggggtagt cggcgaacgc ggcggcgagg gtgcgtacgg ccctggggac
     3781 gtcgtcgcgg gtggcgaggc gcaccgtggg cttgtactcg gtcatggtgg taccccctgg
     3841 ggagagaggt cggtgattcg gtcaacgagg gagccgactg ccgacgtgcg ctccggaggc
     3901 ttgcagaatg cggaacaccg cgcgggcagg aacagggccc acactaccgc cccacacccc
     3961 gcctcccgca ccgccccttc ccggccgctg ctctcggcgc gccctgctga gcagccgcta
     4021 ttggccacag cccatcgcgg tcggcgcgct gccattgctc cctggcgctg tccgtctgcg
     4081 agggtactag tgagacgtgc ggcttccgtt tgtcacgtcc ggcacgccgc gaaccgcaag
     4141 gaaccttccc gacttagggg cggagcagga agcgtcgccg gggggcccac aagggtagcg
     4201 gcgaagatcc gggtgacgct gcgaacggac gtgaagaatg tgcgagaccc agggtcggcg
     4261 ccgctgcgtt tcccggaacc acgcccagag cagccgcgtc cctgcgcaaa cccagggctg
     4321 ccttggaaaa ggcgcaaatc gattgcaaag atggataaag ttttaaacag agaggaatct
     4381 ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca
     4441 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     4501 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     4561 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     4621 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     4681 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     4741 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     4801 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     4861 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     4921 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     4981 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     5041 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     5101 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
     5161 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     5221 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     5281 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     5341 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     5401 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     5461 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     5521 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     5581 tcttccattt caggtgtcgt gacctgcagg atcgtgcggg acgtggacgg aagaaaaaag
     5641 agagtgagcg agcgccggaa tcagcccggc gtggagtgcg gacccgcggt agtgccaggg
     5701 ggcgaaggcg gcggtggtga ggaagatact ttggttagtg accacatcgc agcatgtcct
     5761 tctcccgtgc cctattgtgg gctcggctcc cggcggggcg ccaggctggc caccgggcag
     5821 ccatctgctc tgcccttcgt ccccactttg ggccctttcc cggggttctg gggcaagttt
     5881 ctgtccttgc caccgcctcc tcctctgcct ccggtggctc caaaatacca aacacgtcct
     5941 tgttcgtgcc cctgactgtg aaacctcagg gccccagcgc cgacggcgac gtcggggccg
     6001 agctaacccg gcctctggac aagaatgaag taaagaaggt cttagacaaa ttttacaaga
     6061 ggaaagaaat tcagaaactg ggtgctgatt atggacagaa tcagggcaga gagatgatgc
     6121 tggtgaccag tggagccgta gcctttggca aacaacgctt gcgccatgag atccttctgt
     6181 ctcagagcgt gcggcaggcc ctccactcgg ggcagaacca gctgaaagaa atggcaattc
     6241 cagtcttaga ggcacgagcc tgtgcagctg ccggacagag tgggctgatg gccttgtatg
     6301 aggctatgtt tacccagtac agcatctgtg ctgcccagat tttggtgacc aatttggatt
     6361 tccatgatga gcagaagcgc cggaacctca atggaacact tcatgaactc cttagaatga
     6421 acattgtccc cattgtcaac acaaatgatg ctgttgtccc cccagctgag cccaacagtg
     6481 acctgcaggg ggtaaatgtt attagtgtta aagataatga tagcctggct gcccgactgg
     6541 ctgtggaaat gaaaactgat ctcttgattg ttctttcaga tgtagaaggc ctttttgaca
     6601 gccccccagg ttcagatgat gcaaagctta ttgatatatt ttatcccgga gatcagcagt
     6661 ctgtgacatt tggaaccaag tctagagtgg gaatgggtgg catggaagcc aaggtgaaag
     6721 cagccctctg ggctttgcaa ggtggcactt ctgttgttat tgcaaatgga acccacccaa
     6781 aggtgtctgg gcacgtcatc acagacattg tggaggggaa gaaagttggt accttctttt
     6841 cagaagtaaa gcctgcaggc cctactgttg agcagcaggg agaaatggcg cgatctggag
     6901 gaaggatgtt ggccaccttg gaacctgagc agagaatttc tgtgctggac catgcactag
     6961 aatctttaga taaccttcgg cctggggact gcattgtctg ttttagcaag aatgatattt
     7021 attctgtgag tcggcagatt gaaattcggg gattagaatc agctgttata tatggcagtc
     7081 tcccacctgg gaccaaactt gctcaagcaa aaaagtttaa tgatcccaat gacccatgca
     7141 aaatcttggt tgctacagat gcaattggca tgggacttaa tttgagcata aggagaatta
     7201 ttttttactc ccttataaag cccagtatca atgaaaaggg agagagagaa ctagaaccaa
     7261 tcacaacctc tcaagccctg cagattgctg gcagagctgg cagattcagc tcacggttta
     7321 aagaaggaga ggttacaaca atgaatcatg aagatctcag tttattaaag gaaattttga
     7381 agaggcctgt ggatcctata agggcagctg gtcttcatcc aactgctgag cagattgaaa
     7441 tgtttgccta ccatctccct gatgcaacac tgtccaatct cattgatatt tttgtagact
     7501 tttcacaagt tgatgggcag tattttgtct gcaatatgga tgattttaaa ttttctgcag
     7561 agttgatcca gcatattcca ctaagtctgc gagtgaggta tgttttctgc acagctccta
     7621 tcaacaagaa gcagcctttt gtgtgttctt cactgttaca gtttgccagg cagtatagca
     7681 ggaatgagcc cctgaccttt gcatggttac gccgatacat caaatggcct ttacttccac
     7741 ctaagaatat taaagacctc atggatcttg aagctgtcca cgatgtcttg gatctttact
     7801 tgtggctaag ctaccgattt atggatatgt ttccagatgc cagccttatt cgagatctcc
     7861 agaaagaact agatggtatt atccaagatg gtgtgcacaa tatcactaaa ttgattaaaa
     7921 tgtctgagac gcataagctg ttgaatttgg agggctttcc atcagggagc cagtcacgat
     7981 tgtcaggaac cttaaagagc caagctagaa ggacacgcgg caccaaagct ctagggagta
     8041 aagctactga gccacccagc cccgatgcag gagagctgtc ccttgcttcc agattggtgc
     8101 agcaaggact cctcactcca gacatgctga aacagctaga aaaagagtgg atgacacaac
     8161 aaactgaaca caacaaagaa aaaacagagt ctgggactca tccaaaaggg acgagaagaa
     8221 agaagaagga acctgattcg gactagttag tctgctgaaa caagccgcct gaacgcgtta
     8281 agtcgacaaa gagccaggaa aacccgggaa ttttccaaaa ggtcttcacg ttaaacttgt
     8341 cttatctcag gagagagccc gctcttgtct cccagttcct ggtagggtct gcctgttgga
     8401 aagtgtacct ggatgcttct gggctccgtt tggcaatagc agtcttggct gatgtgcaca
     8461 gtctggctcc cagctcaccc ttttttttta aagtaagaaa atagttgcta ccgataggga
     8521 ctttgccaag tccaattatc ttctaggatt gaaaggtgca ttttccccat aaaaaaggcg
     8581 aggaaaaccc atggctgctt tgtgtcacct cagtgactta cagtccccct tggcatttag
     8641 ttggtactag agccagtcat ccttaacaaa tcttttcaca ttttatttct ttcacatgta
     8701 gtcatcttca aaaaggaaag atttggaatt ttagaaaagg ggcaactctt ctttttagca
     8761 ttctcatcag aaagtcacaa aaatcgatgg aatcatttcc actgggaaga ttgacctttt
     8821 gtatttattt gtggggtaaa ttaataagca ttccagatgc ttgcagcttc ctgcatccag
     8881 gagatgctgt gttccccgtg atgcagctgg aacccaagct gcagcaggag atgcaagttt
     8941 caggatgttc cccactgagc tggaggaata tctacagcag tgatgcttga aatttttgta
     9001 tgaattattt tgtcgtccta cccttttcct ccaaaacaaa aattagagga ttattttaat
     9061 actttggatt cttccccctt ttttgagatt aattaacttt aagaccaatg acttacaagg
     9121 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     9181 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     9241 attggcagaa ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct
     9301 acaagctagt accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc
     9361 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt
     9421 ggaggtttga cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta
     9481 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     9541 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     9601 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     9661 gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     9721 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     9781 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     9841 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     9901 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     9961 ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
    10021 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
    10081 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
    10141 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
    10201 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
    10261 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
    10321 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
    10381 tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
    10441 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
    10501 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
    10561 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
    10621 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
    10681 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
    10741 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
    10801 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt
    10861 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac
    10921 cgaccggctc gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga
    10981 cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc
    11041 ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac
    11101 gaacttccgg gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg
    11161 ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga
    11221 ctgacacgtg ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
    11281 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
    11341 cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    11401 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    11461 catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc
    11521 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
    11581 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
    11641 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    11701 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
    11761 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
    11821 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
    11881 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
    11941 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
    12001 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
    12061 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
    12121 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
    12181 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
    12241 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
    12301 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
    12361 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
    12421 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
    12481 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
    12541 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
    12601 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    12661 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    12721 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    12781 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    12841 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    12901 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    12961 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    13021 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    13081 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    13141 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    13201 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    13261 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    13321 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    13381 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    13441 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    13501 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    13561 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
    13621 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgac
//
