LOCUS       sequence-489173-        9747 bp    DNA     circular SYN 14-OCT-2025
DEFINITION  Expression vector for mRNA production of the click editor construct 
            (CE1; PCV2-nCas9-EcKlenow).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9747)
  AUTHORS   Ferreira da Silva J, Tou CJ, King EM, Eller ML, Rufino-Ramos D, Ma 
            L, Cromwell CR, Metovic J, Benning FMC, Chao LH, Eichler FS, 
            Kleinstiver BP
  TITLE     Click editing enables programmable genome writing using DNA 
            polymerases and HUH endonucleases.
  JOURNAL   Nat Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x.
  PUBMED    39039307
REFERENCE   2  (bases 1 to 9747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jul 22. doi: 10.1038/s41587-024-02324-x."
COMMENT     Sequence Label: Click editor (CE1) mRNA expression vector -
            T7...
FEATURES             Location/Qualifiers
     source          1..9747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..57)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    46..270
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(341..357)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(341..357)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(354..376)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    365..381
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(389..395,396..413,414..419))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    434..455
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(572..589)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(743..1331)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(823..842)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1502..2293,2294..2362))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2125..2144
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2363..2467)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2546..2565)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             join(3268..5781,5782..5784,5785..7368)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             3268..7368
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7426..7446
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9331..9351
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 aaaaaaaaaa aaaagagacg gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
       61 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
      121 ccactgtcct ttcctaataa aatgagaaaa ttgcatcgca ttgtctgagt aggtgtcatt
      181 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
      241 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
      301 cgataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
      361 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
      421 cctagggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
      481 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      541 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
      601 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
      661 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      721 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      781 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      841 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      901 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      961 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1021 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1081 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1141 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1201 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1261 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1321 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacactcag tggaacgaaa
     1381 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1441 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1501 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1561 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     1621 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     1681 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     1741 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     1801 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     1861 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     1921 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     1981 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2041 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2101 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2161 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2221 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2281 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2341 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2401 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2461 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcgatctc
     2521 ccgatcccct agggtctact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     2581 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     2641 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     2701 cgctgcttcg cgatgtacgg gccagatata ggtttagtga accgtcagat ccgctagaga
     2761 tccgcggccg ctaatacgac tcactataag gagaataaac tagtattctt ctggtcccca
     2821 cagactcaga gagaacccgc caccatgagc ccatctaaga aaaacggcag aagcggacct
     2881 caacctcaca agcggtgggt ctttaccctg aacaacccca gcgaagatga gagaaagaag
     2941 atccgggacc tgcctatcag cctgttcgac tacttcatcg tgggcgagga aggaaatgag
     3001 gaaggccgga ccccccacct gcagggcttc gccaacttcg tgaaaaagca gaccttcaac
     3061 aaggtgaagt ggtacctggg cgctagatgc cacatcgaga aggccaaggg caccgaccag
     3121 cagaacaaag agtactgcag caaggaaggc aatctgctga tggaatgtgg cgcccctaga
     3181 tcccagggac agagaagcgg aggctctagc ggctccgaga cacccggcac atctgagtcc
     3241 gccacccctg agagcagcgg cggcagcgac aagaagtaca gcatcggcct ggacatcggc
     3301 accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc
     3361 aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg
     3421 ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac
     3481 accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag
     3541 gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag
     3601 cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac
     3661 cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg
     3721 ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc
     3781 gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac
     3841 aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg
     3901 tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag
     3961 aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac ccccaacttc
     4021 aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac
     4081 gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct gtttctggcc
     4141 gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa caccgagatc
     4201 accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg
     4261 accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc
     4321 gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca ggaagagttc
     4381 tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag
     4441 ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac
     4501 cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc
     4561 ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg
     4621 ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag cgaggaaacc
     4681 atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca gagcttcatc
     4741 gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc caagcacagc
     4801 ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata cgtgaccgag
     4861 ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt ggacctgctg
     4921 ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt caagaaaatc
     4981 gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc ctccctgggc
     5041 acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa tgaggaaaac
     5101 gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag agagatgatc
     5161 gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag
     5221 cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg catccgggac
     5281 aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc caacagaaac
     5341 ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca gaaagcccag
     5401 gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg cagccccgcc
     5461 attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa agtgatgggc
     5521 cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac cacccagaag
     5581 ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa agagctgggc
     5641 agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga gaagctgtac
     5701 ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga catcaaccgg
     5761 ctgtccgact acgatgtgga cgctatcgtg cctcagagct ttctgaagga cgactccatc
     5821 gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa cgtgccctcc
     5881 gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc caagctgatt
     5941 acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag cgaactggat
     6001 aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa gcacgtggca
     6061 cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct gatccgggaa
     6121 gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga tttccagttt
     6181 tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtc
     6241 gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt gtacggcgac
     6301 tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat cggcaaggct
     6361 accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg
     6421 gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac cggggagatc
     6481 gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg
     6541 aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc
     6601 aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa gaagtacggc
     6661 ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc
     6721 aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc
     6781 agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag
     6841 gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga
     6901 atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc ctccaaatat
     6961 gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat
     7021 gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag
     7081 atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc
     7141 gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg
     7201 tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac
     7261 cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc
     7321 accggcctgt acgagacacg gatcgacctg tctcagctgg gaggtgactc cggcggaagc
     7381 tctggtggca gcaagcggac cgccgacggc tctgaattcg agagccctaa gaagaaaaga
     7441 aaggtgagcg gaggctctag cggcggaagc gttatctcct acgacaacta cgtgaccatc
     7501 ctggacgaag agacactgaa ggcttggatc gctaaactgg aaaaagcccc tgtgttcgcc
     7561 ttcgccaccg ccaccgactc cttagataac atcagcgcca acctggtggg cctgtctttc
     7621 gccatcgagc ctggcgtggc cgcctacatc cccgtggccc acgattacct ggatgcccca
     7681 gatcaaatca gcagagagag agcactggag ctcctgaagc ccctgctgga ggacgagaag
     7741 gccctgaagg tgggacagaa cctgaaatac gataggggaa tcctggctaa ttacggcatc
     7801 gagctgagag gcatcgcctt cgataccatg ctggaatctt acatcctgaa cagcgtggcc
     7861 ggccgccacg acatggacag cctggctgaa cggtggctga agcacaaaac catcaccttc
     7921 gaagagatcg ccggaaaggg caagaaccag ctgacattta atcagatcgc actggaagaa
     7981 gccggcagat acgccgctga ggacgccgac gtgacccttc agctgcacct gaagatgtgg
     8041 cccgatctgc agaagcacaa gggcccacta aacgtgttcg agaacatcga gatgcctctg
     8101 gtgcccgtcc tgagcagaat cgagaggaat ggcgtgaaga tcgaccccaa ggtgctgcac
     8161 aaccacagcg aggaactgac actgcggctg gccgagctgg aaaagaaggc ccacgaaatc
     8221 gccggcgagg agttcaacct gtctagcacc aagcagctac aaacaatcct gttcgagaag
     8281 cagggcatta agcctctgaa aaagacacct ggcggagccc ccagcaccag cgaagaagtg
     8341 ctggaggaac tggcgctgga ttaccctctg cccaaagtga tcctggaata tagaggactg
     8401 gccaagctga agtctacata caccgacaag ctgcctctga tgatcaaccc caagaccggc
     8461 cgggtgcata catcttatca ccaggccgtg acagccaccg gccggctgag cagcacagat
     8521 cctaacctgc aaaacatccc agtgcggaac gaagagggca gaagaatcag acaggctttc
     8581 attgcccctg aggactacgt catcgtgtct gctgactaca gccagataga actgagaatc
     8641 atggcccatc tgtctcggga caagggcctg ctgaccgctt ttgcagaagg caaggacatc
     8701 cacagagcca ccgctgccga agtgttcggc ctgcctctgg aaacagtgac cagcgagcaa
     8761 agaaggtccg ccaaggccat taacttcggc ctgatctacg gcatgagcgc ctttggcctg
     8821 gccagacagc tcaacatccc tagaaaggag gcccagaaat acatggacct gtacttcgag
     8881 cggtaccccg gcgtgctgga gtacatggaa cggaccagag cccaggctaa agagcaggga
     8941 tacgtggaaa ccctggatgg ccggagactg tatctgcctg acatcaagag cagcaacggc
     9001 gccagaagag ccgccgctga gagagctgct atcaacgccc ctatgcaggg caccgccgcc
     9061 gacatcatca agcgggccat gatcgccgtg gacgcctggc tgcaggccga gcagcctaga
     9121 gtgcggatga tcatgcaggt gcacgacgag ctggtgtttg aggtgcacaa ggacgacgtg
     9181 gatgctgtgg ccaagcagat tcaccagctg atggaaaatt gcaccagact ggacgtgcca
     9241 ctgctggtcg aggttggaag cggagagaat tgggaccagg cccactctgg cggctcaaaa
     9301 agaaccgccg acggcagcga attcgagtct cccaagaaga agaggaaagt ctaatgactc
     9361 gagctggtac tgcatgcacg caatgctagc tgcccctttc ccgtcctggg taccccgagt
     9421 ctcccccgac ctcgggtccc aggtatgctc ccacctccac ctgccccact caccacctct
     9481 gctagttcca gacacctccc aagcacgcag caatgcagct caaaacgctt agcctagcca
     9541 cacccccacg ggaaacagca gtgattaacc tttagcaata aacgaaagtt taactaagct
     9601 atactaaccc cagggttggt caatttcgtg ccagccacac cctggagcta gcaaaaaaaa
     9661 aaaaaaaaaa aaaaaaaaaa aannnnnnnn naaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     9721 aaaaaaaaaa aaaaaaaaaa aaaaaaa
//
