LOCUS       sequence-489199-         902 bp    DNA     linear   UNA 17-NOV-2025
DEFINITION  Mammalian expression lentiviral vector encoding the 
            Synaptosomal-Associated Protein (SNAP-25) with A1999G mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, 
            Chung WK, Monteggia LM, Brunger AT, Kavalali ET
  TITLE     Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated 
            Encephalopathies.
  JOURNAL   Neuron. 2021 Jan 6;109(1):59-72.e5. doi: 
            10.1016/j.neuron.2020.10.012. Epub 2020 Nov 3.
  PUBMED    33147442
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 17, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2020.10.012"; journalName: "Neuron"; date: 
            "2021-01-6- 6"; volume: "109"; issue: "1"; pages: "59-72.e5"
COMMENT     Sequence Label: pFUW-SNAP25B-A199G
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(800..820)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gactagtaat tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg
       61 gctgcaggtc gactctagag gatcgaattc atggccgagg acgcagacat gcgcaatgag
      121 ctggaggaga tgcagcgaag ggctgaccag ttggctgatg agtcgctgga aagcacccgt
      181 cgtatgctgc aactggttga agagagtaaa gatgctggta tcaggacttt ggttatgttg
      241 gatgaacaag gagaacaact ggaacgcatt gaggaaggga tggaccaaat caataaggac
      301 atgaaagaag cagaaaagaa tttgacggac ctaggaaaat tctgcgggct ttgtgtgtgt
      361 ccctgtaaca agcttaaatc aagtgatgct tacaaaaaag cctggggcaa taatcaggac
      421 ggagtggtgg ccagccagcc tgctcgtgta gtggacgaac gggagcagat ggccatcagt
      481 ggcggcttca tccgcagggt aacaaatgat gcccgagaaa atgaaatgga tgaaaaccta
      541 gagcaggtga gcggcatcat cgggaacctc cgtcacatgg ccctggatat gggcaatgag
      601 atcgatacac agaatcgcca gatcgacagg atcatggaga aggctgattc caacaaaacc
      661 agaattgatg aggccaacca acgtggaaca aagatgctgg gaagtggtta aggatccgtt
      721 aacaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
      781 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
      841 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
      901 gg
//
