LOCUS       sequence-489201-         915 bp    DNA     linear   UNA 20-NOV-2025
DEFINITION  Mammalian expression lentiviral vector encoding the 
            Synaptosomal-Associated Protein (SNAP-25) with D166Y mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, 
            Chung WK, Monteggia LM, Brunger AT, Kavalali ET
  TITLE     Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated 
            Encephalopathies.
  JOURNAL   Neuron. 2021 Jan 6;109(1):59-72.e5. doi: 
            10.1016/j.neuron.2020.10.012. Epub 2020 Nov 3.
  PUBMED    33147442
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2020.10.012"; journalName: "Neuron"; date: 
            "2021-01-6- 6"; volume: "109"; issue: "1"; pages: "59-72.e5"
COMMENT     Sequence Label: pFUW-SNAP25B-D166Y
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(795..815)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacgaag cttgggctgc
       61 aggtcgactc tagaggatcg aattcatggc cgaggacgca gacatgcgca atgagctgga
      121 ggagatgcag cgaagggctg accagttggc tgatgagtcg ctggaaagca cccgtcgtat
      181 gctgcaactg gttgaagaga gtaaagatgc tggtatcagg actttggtta tgttggatga
      241 acaaggagaa caactggaac gcattgagga agggatggac caaatcaata aggacatgaa
      301 agaagcagaa aagaatttga cggacctagg aaaattctgc gggctttgtg tgtgtccctg
      361 taacaagctt aaatcaagtg atgcttacaa aaaagcctgg ggcaataatc aggacggagt
      421 ggtggccagc cagcctgctc gtgtagtgga cgaacgggag cagatggcca tcagtggcgg
      481 cttcatccgc agggtaacaa atgatgcccg agaaaatgaa atggatgaaa acctagagca
      541 ggtgagcggc atcatcggga acctccgtca catggccctg tatatgggca atgagatcga
      601 tacacagaat cgccagatcg acaggatcat ggagaaggct gattccaaca aaaccagaat
      661 tgatgaggcc aaccaacgtg caacaaagat gctgggaagt ggttaaggat ccgttaacaa
      721 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
      781 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
      841 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
      901 ctgtctcttt atgag
//
