LOCUS       sequence-489203-         894 bp    DNA     linear   UNA 20-NOV-2025
DEFINITION  Mammalian expression lentiviral vector encoding the 
            Synaptosomal-Associated Protein (SNAP-25) with G43R mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, 
            Chung WK, Monteggia LM, Brunger AT, Kavalali ET
  TITLE     Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated 
            Encephalopathies.
  JOURNAL   Neuron. 2021 Jan 6;109(1):59-72.e5. doi: 
            10.1016/j.neuron.2020.10.012. Epub 2020 Nov 3.
  PUBMED    33147442
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2020.10.012"; journalName: "Neuron"; date: 
            "2021-01-6- 6"; volume: "109"; issue: "1"; pages: "59-72.e5"
COMMENT     Sequence Label: pFUW-SNAP25B-G43R
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(792..812)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt gggctgcagg
       61 tcgactctag aggatcgaat tcatggccga ggacgcagac atgcgcaatg agctggagga
      121 gatgcagcga agggctgacc agttggctga tgagtcgctg gaaagcaccc gtcgtatgct
      181 gcaactggtt gaagagagta aagatgctcg tatcaggact ttggttatgt tggatgaaca
      241 aggagaacaa ctggaacgca ttgaggaagg gatggaccaa atcaataagg acatgaaaga
      301 agcagaaaag aatttgacgg acctaggaaa attctgcggg ctttgtgtgt gtccctgtaa
      361 caagcttaaa tcaagtgatg cttacaaaaa agcctggggc aataatcagg acggagtggt
      421 ggccagccag cctgctcgtg tagtggacga acgggagcag atggccatca gtggcggctt
      481 catccgcagg gtaacaaatg atgcccgaga aaatgaaatg gatgaaaacc tagagcaggt
      541 gagcggcatc atcgggaacc tccgtcacat ggccctggat atgggcaatg agatcgatac
      601 acagaatcgc cagatcgaca ggatcatgga gaaggctgat tccaacaaaa ccagaattga
      661 tgaggccaac caacgtgcaa caaagatgct gggaagtggt taaggatccg ttaacaattc
      721 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
      781 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
      841 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctgg
//
