LOCUS       sequence-489215-         903 bp    DNA     linear   UNA 20-NOV-2025
DEFINITION  Mammalian expression lentiviral vector encoding the 
            Synaptosomal-Associated Protein (SNAP-25) with V48F mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Alten B, Zhou Q, Shin OH, Esquivies L, Lin PY, White KI, Sun R, 
            Chung WK, Monteggia LM, Brunger AT, Kavalali ET
  TITLE     Role of Aberrant Spontaneous Neurotransmission in SNAP25-Associated 
            Encephalopathies.
  JOURNAL   Neuron. 2021 Jan 6;109(1):59-72.e5. doi: 
            10.1016/j.neuron.2020.10.012. Epub 2020 Nov 3.
  PUBMED    33147442
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 20, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2020.10.012"; journalName: "Neuron"; date: 
            "2021-01-6- 6"; volume: "109"; issue: "1"; pages: "59-72.e5"
COMMENT     Sequence Label: pFUW-SNAP25B-V48F
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(801..821)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg
       61 ggctgcaggt cgactctaga ggatcgaatt catggccgag gacgcagaca tgcgcaatga
      121 gctggaggag atgcagcgaa gggctgacca gttggctgat gagtcgctgg aaagcacccg
      181 tcgtatgctg caactggttg aagagagtaa agatgctggt atcaggactt tgtttatgtt
      241 ggatgaacaa ggagaacaac tggaacgcat tgaggaaggg atggaccaaa tcaataagga
      301 catgaaagaa gcagaaaaga atttgacgga cctaggaaaa ttctgcgggc tttgtgtgtg
      361 tccctgtaac aagcttaaat caagtgatgc ttacaaaaaa gcctggggca ataatcagga
      421 cggagtggtg gccagccagc ctgctcgtgt agtggacgaa cgggagcaga tggccatcag
      481 tggcggcttc atccgcaggg taacaaatga tgcccgagaa aatgaaatgg atgaaaacct
      541 agagcaggtg agcggcatca tcgggaacct ccgtcacatg gccctggata tgggcaatga
      601 gatcgataca cagaatcgcc agatcgacag gatcatggag aaggctgatt ccaacaaaac
      661 cagaattgat gaggccaacc aacgtgcaac aaagatgctg ggaagtggtt aaggatccgt
      721 taacaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga
      781 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
      841 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
      901 tgg
//
