LOCUS       sequence-489241-       10706 bp    DNA     circular SYN 14-OCT-2025
DEFINITION  A piggyBac vector containing the UQCRQ genomic sequence, modified 
            with silent mutations to allow for PCR-based analysis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Luo J, Khandwala S, Hu J, Lee SY, Hickey KL, Levine ZG, Harper JW, 
            Ting AY, Weissman JS
  TITLE     Proximity-specific ribosome profiling reveals the logic of localized
            mitochondrial translation.
  JOURNAL   Cell. 2025 Aug 25:S0092-8674(25)00916-X. doi: 
            10.1016/j.cell.2025.08.002.
  PUBMED    40876456
REFERENCE   2  (bases 1 to 10706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 25:S0092-8674(25)00916-X. doi: 10.1016/j.cell.2025.08.002."
COMMENT     Sequence Label: pJL345
FEATURES             Location/Qualifiers
     source          1..10706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..105)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(131..586)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(268..289)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     480..499
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     713..735
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     727..744
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     728..744
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   874..936
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     misc_feature    1403..1991
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             1509..1520
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1918..1938
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2016..2618)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2102..2122)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2599..2618
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2630..3130)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2693..2711)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     repeat_region   8160..8194
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8685..8701)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8685..8701)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8698..8720)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8709..8725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8733..8739,8740..8757,8758..8763))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8778..8799
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8916..8933)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9087..9675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9167..9186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9846..10637,10638..10706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10469..10488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
      181 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
      241 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
      301 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
      361 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
      421 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
      481 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
      541 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
      601 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      661 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      721 cgacgttgta aaacgacggc cagtgagcgc gcctcgttca ttcacgtttt tgaacccgtg
      781 gaggacgggc agactcgcgg tgcaaatgtg ttttacagcg tgatggagca gatgaagatg
      841 ctcgacacgc tgcagaacac gcagctagat taaccctaga aagataatca tattgtgacg
      901 tacgttaaag ataatcatgt gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
      961 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1021 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaac aaaaactcaa
     1081 aatttcttct ataaagtaac aaaactttta tgagggacag ccccccccca aagcccccag
     1141 ggatgtaatt acgtccctcc cccgctaggg ggcagcagcg agccgcccgg ggctccgctc
     1201 cggtccggcg ctccccccgc atccccgagc cggcagcgtg cggggacagc ccgggcacgg
     1261 ggaaggtggc acgggatcgc tttcctctga acgcttctcg ctgctctttg agcctgcaga
     1321 cacctggggg gatacgggga aaaggcctcc agggtttatt acagggacag cagagatcca
     1381 gtttggttaa ttagctagcg atgcggggag gcggcccaaa gggagatccg actcgtctga
     1441 gggcgaaggc gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg
     1501 tccgctggat tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg
     1561 gcaacacagg cgagcagcca aggaaaggac gatgatttcc ccgacaacac cacggaattg
     1621 tcagtgccca acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc
     1681 gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca
     1741 atgccccaac cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg
     1801 acaacgggcc acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa
     1861 atgaaagcca tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat
     1921 agcgtaaaag gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc
     1981 agaggttgat taccgataag cttgatatcg aattcttaac tggcaccggg cttgcgggtc
     2041 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     2101 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     2161 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     2221 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     2281 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     2341 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     2401 acggcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     2461 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     2521 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     2581 cgggtggcga ggcgcaccgt gggcttgtac tcggtcatgg tggtaccccc tggggagaga
     2641 ggtcggtgat tcggtcaacg agggagccga ctgccgacgt gcgctccgga ggcttgcaga
     2701 atgcggaaca ccgcgcgggc aggaacaggg cccacactac cgccccacac cccgcctccc
     2761 gcaccgcccc ttcccggccg ctgctctcgg cgcgccctgc tgagcagccg ctattggcca
     2821 cagcccatcg cggtcggcgc gctgccattg ctccctggcg ctgtccgtct gcgagggtac
     2881 tagtgagacg tgcggcttcc gtttgtcacg tccggcacgc cgcgaaccgc aaggaacctt
     2941 cccgacttag gggcggagca ggaagcgtcg ccggggggcc cacaagggta gcggcgaaga
     3001 tccgggtgac gctgcgaacg gacgtgaaga atgtgcgaga cccagggtcg gcgccgctgc
     3061 gtttcccgga accacgccca gagcagccgc gtccctgcgc aaacccaggg ctgccttgga
     3121 aaaggcgcaa acgcgttgga ttaagatgtt cgctaggtgc ttgctgaaac ttctaaggca
     3181 tgtggaaaag ctaaccaatt attcatgacg atactcagat ctgctggtat gcaacttatg
     3241 gtattaggtt tactcttcct ctcaatttat cctcacggcc ttttccactt ccaggtgttt
     3301 acaattgtaa ttattcgctg catgttaatt tgcgaattta caaatttggg tgagaaagtc
     3361 acaattggca tagaagttcc agaaagcaga aaaccattag gtctctcagt cctcccaggt
     3421 ggctgggtac agaatacgta cttggagtac tggaattgta tttgcgatgt tcttaaaaga
     3481 ggacaccgcc tttaaccttg acttattttt aaatgtaaca tactgatgtc cttactttgg
     3541 tctgcacact catctataca tattttggtt gaaaggtttt gggatgtttg cagatgctat
     3601 aaaataaatg aacgttttat tattattcat ccaaatggtg gtagaatttg ccacaatatt
     3661 tctgtcctta cctgtatttc tgttcttgcc ttggagctga gaccaggcta gacattatgg
     3721 tttaaaaaaa tgaggcaaag gagcaccatg aaaagctgaa atgaggggat attcagcgcc
     3781 accccgaagg agactatgct ggaagagtca gcctacactc atagtgcaag atacgaagcc
     3841 agtttgtgca gtacctacat gagaaaacta aggtatgtct gcaaaataca agttgactgc
     3901 ctgccgagcc tccgtggtcc gtaagtagtg ttataaaaca cagccaactg ttgaatgaga
     3961 acttagtcct agataaaata tggccagcaa ttccattaac aaaacactga caagacacct
     4021 cagggcgagc tgtacattga caacagcatc gtcaagcaga atgcacttca gggaaaacat
     4081 aataaggcct ctcacccgac tttccttgct tctcacgtgg ggagaagaaa gaactggggc
     4141 atcgtgattc cccccacccc acgcaaacca tctggggagg acgctggctg ctgtcgaggc
     4201 ggcctcagca ttcatcaatt gacgattatt tgattatttt tgtggctgaa actgacgaaa
     4261 cgtttgtatt atctcttggg aacgttaaca gcctgccagg ggactctaaa acagagtcca
     4321 agagaagcct tcgatatgtg tccctgattt tggtgctggg gtcgctcatg gcctgaaaat
     4381 aacgaagccc cctttgagct actggccaag gccaaataaa acctcgtgct cctctgctgt
     4441 ggggcggccc ggcctcgggg ctcagaggcg ctctgtctac agctgggaag actcgcaggc
     4501 cccaagctcc tggccagcgc tgccccggag gtcggcaggc cccttcctcg tcaccttttt
     4561 gttccctccc ccgcctcccg cattcggccg cttcctgact gggattccac agaaaagccg
     4621 agggctgagg agaagtgtga gcgcctccgc ctgtccactg tcccccaaag tcagttcaat
     4681 ccccgacgtc ctccgctagg ctccacccca ccggcccggg cagggcctcc aaggcacctc
     4741 ccacctacgg gtcacccagt cagcccactt ctttctggga caaaggcgtc atcccttaga
     4801 gacagtagga aaatggtatc tcccggaagt tacctcacga cctccaagag cggcttccaa
     4861 ccttgccgga aatgacgaac gagtcaaccg gatcggtgac tgtggagggc gagctgagcc
     4921 ctgtgcgtga gtggggtctg gctgtgcagt gttggtggac cctgggaggc taggggcgcc
     4981 ccgctgggct gggaaaggat aaggagtgca ggggcaggag tctggggttg gggatggacc
     5041 cccgcgggga ctgcggcgct tcgcgaaagc gagccaagcg cctgtccacc ctcggtcctg
     5101 cagggccgcc gccacaatgg gccgcgagtt tgggaatctg acgcggatgc ggcatgtcat
     5161 ctcctactcc ttgagtccat tcgagcagcg cgcctatccg cacgtcttca ctaaaggaat
     5221 ccccaatgtt ctgcgccgca ttcgggagtc tttctttcgc gtggtgccgc gtgagtgcct
     5281 tgggcccgcg ggagcgggag gctggacccc agcagcagca gcagtcactg cgcctcccct
     5341 ctgagcgctg gcggccgggc aggcgctctg taacttgccg tacacacctt tctcattgaa
     5401 tattcctgac cgcccttaag ctggtcatat tatcctcccc atcttacacg gggaaaactg
     5461 agctacagaa ctgaaatgac tggcccaagg tcactcgggt gattccagca tgttcctctg
     5521 aggtcacctt tatttagttc ttaccagtgg tgaaatgctt tgtgttaaac gtgcagcaga
     5581 tagtgatgat agttgcatgg ccttggatat gctaagaact actgaagtgt atgctttaaa
     5641 gaagacaaga ggactgagac cttttttttt cttctatatg tcttatatgt gtgctctctg
     5701 tttactctct taatttttaa agagtttgta gtgttttatc ttatctacac atgggggact
     5761 gaagagttcg agagatccaa gaggaagaat ccagctgcct atgaaaatga caaatgagca
     5821 acgcatccgg atgacggttc cctgtctctg aaagaccttt ctctggaaga ggagtctgca
     5881 ttgtagtgtc tcaaagacac aataaacttc ctatggtctg cactgttgtg atattacatt
     5941 tttgtgagta gaatcctgtg tgaccactaa tattcaagtt catgaagcgc ccctcctcag
     6001 catgagtctt gaagttttgc ctggtctcgg gttttggtga aagagcaggg ctcccccttt
     6061 gttccattat ctacaagaaa gtagctgcag gtactcctgc atctaaccat ttgtgggtga
     6121 catgggctat ggcgagtgct ggttcttggc cctctgctcc catagcaaca ataaagtcta
     6181 ctaactatat tgtaattagt atgtgtcagg gacgctttta ttttttattt tttgagatgg
     6241 agtcttgctc tctcacccag gctggagtgc agtggtgtga tctcggctca ctgccatctg
     6301 tgcctcctgg gttcaaacga ttctcctgtc tcagcctccg agtagctggg attacaggtg
     6361 caggacacca cacctggcta attttttgta tttttagtag agacaggttt caccatgttg
     6421 gccaggctgg tctggaactc ctgacctcag atgatctgtg cgcctcagcc tcccaaagtg
     6481 ctgggattac aggcgtaagc catggtgctc tgggcctcaa atactcttct tagctacaca
     6541 aatgaactgt taaacctcac aagcaccttt aacgtatcct catttggcag atgaggaaat
     6601 gggcacagag agattaagta atttgccaga aattatacag cagtgaatgg tggagtcttg
     6661 cactaaagtg atgctcttaa aactatgctc tattgcaggg gtgtccaatc ttttagcttc
     6721 cctgggccac acataaaata cgctaacatt acaatagctg ataagcttta aaaaatggca
     6781 aaaaaaaccc tcagcgtttt agaaagttta caaatttttg ttgggtcgga ttcaaagcca
     6841 tcctgggctg cttgctgccc aggactgcgg ttggacaagc ttgctctact gcctttcctc
     6901 ctaaggcagg ccttagcctg aggggatatc agctataaaa cggatgaggt gatctcttag
     6961 cctccacagc agttttctgt ttctgtgcca gaggccaaca atgttttcga ccatatgtgg
     7021 ttcataaagc ctcaaagatt tactctggcc ctttatgaat aaagtttacc cctgatctaa
     7081 agcctttaaa ttatcttttt ggtattaaag ataccatttt cccttcgccc accgagagat
     7141 ggtgggggta ggtgtggttg gcagttttat gcctctagtt ccttagagag gcttctctct
     7201 aagcttcggg acatggatct cacctgtggt agcaggtatg tggaatatga tgagttgtca
     7261 tagctcagtc agtacagtat atgctatggg atctgagatc cgtttttaga aactcttcta
     7321 aggactcttc ctgctagcag caactttgat tctgggtcag actgtgtttt tttttttttt
     7381 ttttttttga gatgggagtc ttgctctgtc accaggctgg agtttagtgg cgcaatctca
     7441 gcttactgca acctccgctt cccgggctca agtgattctc ctgcctcagc ctcccaaata
     7501 gctgggacta caggcgcata ccaacacacc cagctaattt ttgtattttt attttttttt
     7561 ttagtagaga cggagtttca ccatgttggc caggatggtc ttgatctctt gacctcgtga
     7621 tccacctgcc tcgacctccc aaagtgctgg gattacaggt gtgagccacc gtgcctggcc
     7681 agactgtatc tttattatcc taattactgc tattgccttt taagaatcaa aagttaagat
     7741 acttcaaaac atggccagat acagtggctc acacctgtaa tcccagcact ttgggaggct
     7801 gaagtggatg gatcacttga ggtcaggagt ttgagaccag cctggccaac atggtgaaac
     7861 cctgtctcta ctaaaaatat aaaaattagc tgagtgtggt ggcacacgcc tataatccca
     7921 gctactcagg aggctgaggc agaaacgctt gaacctggga ggtggaggtt gcagtgagcc
     7981 aagattgtgc cactgcactc cagcctgggt gacagagcga gacttcaaaa caaaacctaa
     8041 aagataaact acattgtgag gcaggatacg gggtttaaac caagcggcga ctgagatgtc
     8101 ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc
     8161 atgcgtcaat tttacgcaga ctatctttct agggttaatc tagctgcatc aggatcatat
     8221 cgtcgggtct tttttccggc tcagtcatcg cccaagctgg cgctatctgg gcatcgggga
     8281 ggaagaagcc cgtgcctttt cccgcgaggt tgaagcggca tggaaagagt ttgccgagga
     8341 tgactgctgc tgcattgacg ttgagcgaaa acgcacgttt accatgatga ttcgggaagg
     8401 tgtggccatg cacgccttta acggtgaact gttcgttcag gccacctggg ataccagttc
     8461 gtcgcggctt ttccggacac agttccggat ggtcagcccg aagcgcatca gcaacccgaa
     8521 caataccggc gacagccgga actgccgtgc cggtgtgcag attaatgaca gcggtgcggc
     8581 gctgggatat tacgtcagcg aggacgggta tcctggctgg atgccgcaga aatggacatg
     8641 gataccccgt gagttacccg gcgggcgcgc ttggcgtaat catggtcata gctgtttcct
     8701 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     8761 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     8821 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8881 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8941 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     9001 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     9061 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9121 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9181 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9241 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9301 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9361 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9421 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9481 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9541 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     9601 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9661 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9721 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9781 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9841 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9901 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9961 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    10021 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    10081 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    10141 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    10201 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    10261 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    10321 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    10381 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    10441 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    10501 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    10561 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    10621 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    10681 gcgacacgga aatgttgaat actcat
//
