LOCUS       sequence-48925-e        1027 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  TITLE     SGC+year
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: PIM2
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgt tgaccaagcc tctacagggg cctcccgcgc cccccgggac ccccacgccg
      121 ccgccaggag gcaaggatcg ggaagcgttc gaggccgagt atcgactcgg ccccctcctg
      181 ggtaaggggg gctttggcac cgtcttcgca ggacaccgcc tcacagatcg actccaggtg
      241 gccatcaaag tgattccccg gaatcgtgtg ctgggctggt cccccttgtc agactcagtc
      301 acatgcccac tcgaagtcgc actgctatgg aaagtgggtg caggtggtgg gcaccctggc
      361 gtgatccgcc tgcttgactg gtttgagaca caggagggct tcatgctggt cctcgagcgg
      421 cctttgcccg cccaggatct ctttgactat atcacagaga agggcccact gggtgaaggc
      481 ccaagccgct gcttctttgg ccaagtagtg gcagccatcc agcactgcca ttcccgtgga
      541 gttgtccatc gtgacatcaa ggatgagaac atcctgatag acctacgccg tggctgtgcc
      601 aaactcattg attttggttc tggtgccctg cttcatgatg aaccctacac tgactttgat
      661 gggacaaggg tgtacagccc cccagagtgg atctctcgac accagtacca tgcactcccg
      721 gccactgtct ggtcactggg catcctcctc tatgacatgg tgtgtgggga cattcccttt
      781 gagagggacc aggagattct ggaagctgag ctccacttcc cagcccatgt ctccccagac
      841 tgctgtgccc taatccgccg gtgcctggcc cccaaacctt cttcccgacc ctcactggaa
      901 gagatcctgc tggacccctg gatgcaaaca ccagccgagg atgtacccct caacccctcc
      961 aaaggaggcc ctgccccttt ggcctggtcc ttgctaccct gacagtaaag gtggatacgg
     1021 atccgaa
//
